小鼠的下丘脑室旁核向前包钦格复合体直接投射参与调控呼吸

刘凌琳; 张俊鸿; 熊玮

doi:10.16016/j.2097-0927.202408088

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小鼠的下丘脑室旁核向前包钦格复合体直接投射参与调控呼吸

神经科学 | 更新时间：2026-01-09

- 小鼠的下丘脑室旁核向前包钦格复合体直接投射参与调控呼吸
- Paraventricular hypothalamic nucleus projects directly to the preBötzinger complex and is involved in regulation of respiration in mice
- 陆军军医大学学报 2024年46卷第20期页码：2265-2275
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院老年医学与特勤医学科
- 作者简介：
  
  [熊玮，E-mail: xiongwei@tmmu.edu.cn XIONG Wei, E-mail: xiongwei@tmmu.edu.cn
- 基金信息：
  
  ‍国家重点研发计划(2018YFC2000301)
- DOI：10.16016/j.2097-0927.202408088
  中图分类号： R338.2; R322.8; R563.8
- 收稿：2024-08-21，
  
  修回：2024-08-31，
  
  纸质出版：2024-10-30
- 稿件说明：
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刘凌琳, 张俊鸿, 熊玮. 小鼠的下丘脑室旁核向前包钦格复合体直接投射参与调控呼吸[J]. 陆军军医大学学报, 2024,46(20):2265-2275.

Linglin LIU, Junhong ZHANG, Wei XIONG. Paraventricular hypothalamic nucleus projects directly to the preBötzinger complex and is involved in regulation of respiration in mice[J]. Journal of Army Medical University, 2024, 46(20): 2265-2275.
刘凌琳, 张俊鸿, 熊玮. 小鼠的下丘脑室旁核向前包钦格复合体直接投射参与调控呼吸[J]. 陆军军医大学学报, 2024,46(20):2265-2275. DOI： 10.16016/j.2097-0927.202408088.

Linglin LIU, Junhong ZHANG, Wei XIONG. Paraventricular hypothalamic nucleus projects directly to the preBötzinger complex and is involved in regulation of respiration in mice[J]. Journal of Army Medical University, 2024, 46(20): 2265-2275. DOI： 10.16016/j.2097-0927.202408088.

摘要

目的

探究小鼠全脑呼吸相关核团的空间分布，以及下丘脑室旁核(paraventricular hypothalamic nucleus，PVN)与前包钦格复合体(preBötzinger complex，preBötC)之间的环路连接及投射关系。

方法

运用从外周到中枢逆行示踪的研究策略，在小鼠的膈肌微量注射伪狂犬病毒(pseudorabies virus，PRV)，逆行标记脊髓呼吸膈运动神经元的上游输入脑区。在PVN微量注射顺行不跨突触病毒(AAV2/9-EGFP)，标记PVN下游脑区；并利用Cre/loxP基因表达策略，在PVN及preBötC分别注射Dio-EGFP及Retro-Cre病毒，验证PVN-preBötC环路及二者之间的投射关系。

结果

PRV介导的逆行示踪结果显示：被PRV-EGFP标记的阳性神经元分布在PVN、preBötC、孤束核(nucleus tractus solitarii

NTS)、蓝斑(locus coeruleus

LC)和中脑导水管周围灰质(periaqueductal gray，PAG)等多个脑区，除延髓区域外，PVN脑区的PRV-EGFP阳性神经元占比最高(

＜0.000 1)。在PVN注射AAV2/9-EGFP，经病毒感染后在preBötC脑区可见绿色神经纤维。在PVN和preBötC分别注射Dio-EGFP及Retro-Cre病毒，经病毒感染后PVN脑区可见绿色神经元胞体。

结论

PVN是脊髓呼吸膈运动神经元上游重要的输入核团，并有可能通过PVN-preBötC环路的直接投射调控呼吸。

Abstract

Objective

To probe the spatial distribution of respiratory-related nuclei throughout the whole brain

and to investigate the circuit and projection links between the paraventricular hypothalamic nucleus (PVN) and the preBötzinger complex (preBötC) in mice.

Methods

Using a retrograde tracing strategy from peripheral to central

pseudorabies viruses (PRV) were microinjected into the diaphragm of mice to retrogradely label the upstream inputs brain regions from spinal respiratory phrenic motor neurons. The anterograde non-transsynaptic tracing viruses (AAV2/9-EGFP) were microinjected into the PVN to label the downstream brain region of the PVN; And using the Cre/loxP gene expression strategy

Dio-EGFP and Retro-Cre viruses were injected in the PVN and preBötC

respectively

to validate the PVN-preBötC circuit and the projection relationship between the two.

Results

The results of PRV-mediated retrograde tracing showed that PRV-EGFP-positive neurons were distributed in several brain regions such as PVN

preBötC

nucleus tractus solitarii (NTS)

locus coeruleus (LC) a

nd periaqueductal grey (PAG). Except for the medulla oblongata region

the PVN brain region had the highest percentage of PRV-EGFP-positive neurons (

0.000 1). After AAV2/9-EGFP viruses were injected in the PVN

green neurofibrils were observed in the preBötC brain region after viral infection; Dio-EGFP and Retro-Cre viruses were injected in the PVN and preBötC

respectively

and green neuronal cells were seen in the brain region of the PVN after viral infection.

Conclusion

PVN is an important upstream input nucleus of spinal respiratory phrenic motor neurons

and has the potential to regulate respiration through direct projections from PVN-preBötC circuit.

关键词

Keywords

references

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