Ⅰ型糖尿病小鼠背部皮肤生物力学性能退化和伤口愈合障碍的机制研究

范德江; 贺伟峰

doi:10.16016/j.2097-0927.202407016

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Ⅰ型糖尿病小鼠背部皮肤生物力学性能退化和伤口愈合障碍的机制研究

基础医学 | 更新时间：2026-01-09

- Ⅰ型糖尿病小鼠背部皮肤生物力学性能退化和伤口愈合障碍的机制研究
- Degradation of biomechanical properties and impaired wound healing in the back skin of mice with type 1 diabetes mellitus: a quantitative study
- 陆军军医大学学报 2024年46卷第21期页码：2383-2396
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院烧伤研究所，重庆市创面组织修复与再生重点实验室
- 作者简介：
  
  [贺伟峰，E-mail: heweifeng@tmmu.edu.cn HE Weifeng, E-mail: heweifeng@tmmu.edu.cn
- 基金信息：
  
  国家自然科学基金(82172232);重庆市自然科学基金(CSTB2024NSCQ-LZX0057)
- DOI：10.16016/j.2097-0927.202407016
  中图分类号： R322.991;R587.1;R64
- 收稿：2024-07-04，
  
  修回：2024-08-21，
  
  纸质出版：2024-11-15
- 稿件说明：
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范德江, 贺伟峰. Ⅰ型糖尿病小鼠背部皮肤生物力学性能退化和伤口愈合障碍的机制研究[J]. 陆军军医大学学报, 2024,46(21):2383-2396.

Dejiang FAN, Weifeng HE. Degradation of biomechanical properties and impaired wound healing in the back skin of mice with type 1 diabetes mellitus: a quantitative study[J]. Journal of Army Medical University, 2024, 46(21): 2383-2396.
范德江, 贺伟峰. Ⅰ型糖尿病小鼠背部皮肤生物力学性能退化和伤口愈合障碍的机制研究[J]. 陆军军医大学学报, 2024,46(21):2383-2396. DOI： 10.16016/j.2097-0927.202407016.

Dejiang FAN, Weifeng HE. Degradation of biomechanical properties and impaired wound healing in the back skin of mice with type 1 diabetes mellitus: a quantitative study[J]. Journal of Army Medical University, 2024, 46(21): 2383-2396. DOI： 10.16016/j.2097-0927.202407016.

摘要

目的

探究由链脲佐菌素(streptozocin

STZ)诱导的Ⅰ型糖尿病(type Ⅰ diabetes mellitus，T1DM)小鼠，其背部皮肤生物力学性能减弱和伤口愈合障碍的机制。

方法

40只8周龄且体质量为20~25 g的雄性C57BL/6小鼠，采用随机数字表法分为2组：野生型(wild type，WT)组(

=20)和T1DM组(

=20)。利用原子力显微镜和扫描电镜检测皮肤的杨氏模量、松弛率及胶原排布。将皮肤组织石蜡切片进行苏木精-伊红(HE)染色、马松染色、天狼星红染色、Gordon Sweet染色和维多

利亚蓝染色，分别评估表皮与肉芽组织、总胶原含量、Ⅲ型胶原与Ⅰ型胶原比例、网状纤维含量和弹性纤维含量。免疫组织化学染色法和蛋白免疫印迹法定量检测Ⅰ型胶原与Ⅲ型胶原蛋白表达情况。流式细胞术和组织石蜡切片的免疫荧光染色检测创面组织内肌成纤维细胞转化情况。

结果

与WT组相比，在造创后第21天T1DM组小鼠背部皮肤刚度、强度和回弹力均显著降低(

＜0.05)；愈合过程中及愈合后T1DM组胶原蛋白、弹性纤维减少，Ⅲ型胶原蛋白与Ⅰ型胶原蛋白比例显著增加，网状纤维明显增多(

＜0.05)；T1DM组创面愈合早期阶段肌成纤维细胞转化显著减少(

＜0.05)。

结论

T1DM抑制成纤维细胞向肌成纤维细胞转化，引起伤口愈合障碍，导致小鼠背部皮肤力学性能减弱。

Abstract

Objective

To investigate the mechanisms underlying the reduced biomechanical properties and impaired wound healing in the dorsal skin of streptozocin (STZ)-induced type 1 diabetes mellitus (T1DM) mice.

Methods

Forty male C57BL/6 mice (8 weeks old

weighing 20~25 g) were randomly divided into wild-type (WT

=20) and T1DM (

=20) groups. After the mice were inflicted with full-thickness skin resection (circular

1 cm in diameter

in both sides of the back midline)

atomic force microscopy (AFM) and scanning electron microscopy (SEM) were employed to assess the Young's modulus

relaxation rate

and collagen arrangement of the skin

and HE

Masson's trichrome

Sirius red

Gordon-Sweet

and Victoria blue staining were all applied to evaluate the epidermis and granulation tissue

total collagen content

ratio of type Ⅲ to type Ⅰ collagen

reticular fiber content

and elastic fiber content. Immunohistochemical assay and Western blotting were conducted to quantify the expression of type Ⅰ and type Ⅲ collagen proteins. Flow cytometry and immunofluorescence staining of paraffin sections were performed to detect the transformation of myofibroblast in wound tissues.

Results

On day 21 post-wounding

the dorsal skin of T1DM mice exhibited significantly reduced stiffness

strength

and resilience compared to the conditions in the WT group (

0.05). During and aft

er healing

T1DM mice showed decreased collagen and elastic fibers

an increased ratio of type Ⅲ to type Ⅰ collagen

and increased reticular fibers

all with statistical significance (

0.05). Additionally

there was a significant reduction in myofibroblast transformation during the early stages of wound healing in the T1DM group (

0.05).

Conclusion

T1DM inhibits the transformation of fibroblasts into myofibroblasts

leading to impaired wound healing and reduced biomechanical properties in the dorsal skin of mice.

关键词

Keywords

references

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