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H-NS蛋白对泰国伯克霍尔德菌毒力作用的初探
Effect of H-NS protein on the virulence of
Burkholderia thailandensis - 陆军军医大学学报 2024年46卷第23期 页码：2581-2587
- 作者机构：
400042 重庆，陆军特色医学中心检验科
- 作者简介：
[ 鲁卫平, E-mail：luwp@tmmu.edu.cnLU Weiping, E-mail: luwp@tmmu.edu.cn
- 基金信息：国家自然科学基金面上项目(82072348)
DOI：10.16016/j.2097-0927.202406080
中图分类号： R378.99;R446.5;R915收稿：2024-06-25，
修回：2024-10-25，
纸质出版：2024-12-15
- 稿件说明：
移动端阅览
卫蕾蕾, 莫非, 王春燕, 等. H-NS蛋白对泰国伯克霍尔德菌毒力作用的初探[J]. 陆军军医大学学报, 2024,46(23):2581-2587.
Leilei WEI, Fei MO, Chunyan WANG, et al. Effect of H-NS protein on the virulence of
Burkholderia thailandensis [J]. Journal of Army Medical University, 2024, 46(23): 2581-2587.卫蕾蕾, 莫非, 王春燕, 等. H-NS蛋白对泰国伯克霍尔德菌毒力作用的初探[J]. 陆军军医大学学报, 2024,46(23):2581-2587. DOI： 10.16016/j.2097-0927.202406080.
Leilei WEI, Fei MO, Chunyan WANG, et al. Effect of H-NS protein on the virulence of
Burkholderia thailandensis [J]. Journal of Army Medical University, 2024, 46(23): 2581-2587. DOI： 10.16016/j.2097-0927.202406080.
摘要
目的
2
构建泰国伯克霍尔德菌BPM的
hns
基因过表达菌株，探究
hns
基因过表达对细菌生长、运动、生物被膜形成能力和抗菌药物敏感性的影响。
方法
2
将
hns
基因03253和05307分别克隆至表达载体pTAC-Cm上，获得重组载体pTAC- 03253和pTAC- 05307；将两种质粒分别电转至BPM感受态菌株中获得重组菌株，对其生长、运动、生物被膜和药物敏感性的表型变化进行检测。
结果
2
成功获得
hns
基因过表达菌株；和野生株BPM相比，
hns
基因过表达菌株的生长和运动能力受到明显抑制，生物被膜形成能力显著增强，对左氧氟沙星和米诺环素的敏感性下降。
结论
2
H-NS蛋白在泰国伯克霍尔德菌BPM毒力和耐药性的调控中发挥重要作用，可成为泰国伯克霍尔德菌感染诊治的新靶点。
Abstract
Objective
2
To construct
hns
gene overexpression strains of
Burkholderia thailandensis
BPM
and explore the effects of
hns
overexpression on bacterial growth
motility
biofilm formation ability and susceptibility to antimicrobial drugs.
Methods
2
The
hns
genes 03253 and 05307 were respectively cloned into the expression vector pTAC-Cm to yield recombinant vectors pTAC-03253 and pTAC-05307. Then the 2 types of plasmids were electroporated into the BPM competent strain to obtain recombinant strains
and their phenotypic changes in growth
motility
biofilm
and drug sensitivity were detected.
Results
2
The strains of
hns
overexpression were successful constructed
and they presented significantly inhibited growth and motility
markedly enhanced ability to
form biofilms
decreased sensitivity to levofloxacin and minocycline when compared with the wild-type BPM strain.
Conclusion
2
H-NS protein plays an important role in the regulation of virulence and drug resistance in
Burkholderia thailandensis
BPM
and can become a new target for the treatment of infections caused by
Burkholderia thailandensis
.
关键词
Keywords
references
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