全肠外营养模型小鼠肠道真菌多样性及构成改变

谢会超; 陈弈辉; 刘星宇; 韩奔; 孙力华; 肖卫东

doi:10.16016/j.2097-0927.202404125

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全肠外营养模型小鼠肠道真菌多样性及构成改变

基础医学 | 更新时间：2026-01-09

- 全肠外营养模型小鼠肠道真菌多样性及构成改变
- Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition
- 陆军军医大学学报 2024年46卷第21期页码：2407-2414
- 作者机构：
  
  1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院普通外科
  2.400037 重庆，陆军军医大学(第三军医大学)第二附属医院营养科
- 作者简介：
  
  [肖卫东，E-mail: xiaoweidong@tmmu.edu.cn XIAO Weidong, E-mail: xiaoweidong@tmmu.edu.cn
- 基金信息：
  
  国家自然科学基金面上项目(82270585);军事医学高新技术培育项目(CX2019JS212)
- DOI：10.16016/j.2097-0927.202404125
  中图分类号： Q949.32;R-332;R459.3
- 收稿：2024-04-26，
  
  修回：2024-06-27，
  
  纸质出版：2024-11-15
- 稿件说明：
移动端阅览
谢会超, 陈弈辉, 刘星宇, 等. 全肠外营养模型小鼠肠道真菌多样性及构成改变[J]. 陆军军医大学学报, 2024,46(21):2407-2414.

Huichao XIE, Yihui CHEN, Xingyu LIU, et al. Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition[J]. Journal of Army Medical University, 2024, 46(21): 2407-2414.
谢会超, 陈弈辉, 刘星宇, 等. 全肠外营养模型小鼠肠道真菌多样性及构成改变[J]. 陆军军医大学学报, 2024,46(21):2407-2414. DOI： 10.16016/j.2097-0927.202404125.

Huichao XIE, Yihui CHEN, Xingyu LIU, et al. Diversity and composition changes of intestinal Fungi in a mouse model of total parenteral nutrition[J]. Journal of Army Medical University, 2024, 46(21): 2407-2414. DOI： 10.16016/j.2097-0927.202404125.

摘要

目的

研究小鼠在全肠外营养(total parenteral nutrition，TPN)模型下与正常小鼠相比，肠道中真菌群落的多样性以及组成结构的改变。

方法

建立TPN小鼠模型后，采集TPN模型小鼠(

=5)和正常对照小鼠(

=5)的新鲜粪便样本，利用ITS DNA测序技术对肠道真菌进行测定，然后通过生物信息学方法对两组小鼠的真菌菌群多样性、群落结构及功能特征的差异进行分析。

结果

TPN模型小鼠和正常对照小鼠的肠道真菌Alpha多样性具有明显的差异(

＜0.05)；Beta多样性存在统计学差异(

＜0.01)；在TPN模型小鼠中，

Candida

(假丝酵母属)的相对丰度明显减少(

＜0.01)，

Penicillium

(青霉菌属)的相对丰度明显减少(

＜0.01)，

Aspergillus

(曲霉菌属)的相对丰度明显减少(

＜0.01)，

Talaromyces

(

篮状菌属)的相对丰度明显减少(

＜0.01)。LEfSe分析揭示，在正常对照小鼠中，

Candida

(假丝酵母属)，

Penicillium

(青霉菌属)，

Aspergillus

(曲霉菌属)和

Talaromyces

(篮状菌属)有明显的富集。

结论

与正常小鼠相比，TPN模型小鼠的肠道真菌菌群组织结构发生明显改变。肠道真菌菌群失调可能促进TPN相关并发症的发生，调节肠道真菌群落平衡可能成为预防TPN相关并发症的一种新策略。

Abstract

Objective

To analyze the diversity and composition changes of gut fungal communities between mouse model of total parental nutrition (TPN) and normal control mice.

Methods

After mouse model of TPN was constructed

fresh feces were collected from TPN mice (

=5) and normal control mice (

=5). Internal transcribed spacer (ITS) DNA sequencing was applied to determine intestinal fungi

and then bioinformatics analysis was conducted to identify the differences in fungal diversity

structure

and functional properties between the 2 groups of mice.

Results

There were significant differences in Alpha diversity (

0.05) and Beta diversity (

0.01) of intestinal fungi between the 2 groups. In the TPN model group

the relative abundances of

Candida

Penicillium

Aspergillus

and

Talaromyces

were obviously reduced (all

0.01). LEfSe analysis indicated that the above 4 strains were notably enriched in the normal control mice.

Conclusion

TPN mice exhibit characteristic changes in the composition of gut fungal flora compared to normal control mice. Dysfunction of gut fungal community may promote the occurrence of TPN related complications

and regulating the balance of gut fungal community may become a new strategy for preventing TPN related complications.

关键词

Keywords

references

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