浏览全部资源
扫码关注微信
肿瘤相关性贫血与SARS-CoV-2特异性记忆CD8+T细胞丰度的相关性分析
Correlation between cancer related anemia and abundance of SARS-CoV-2 specific memory CD8+ T cells
- 陆军军医大学学报 2024年46卷第9期 页码：1047-1056
- 作者机构：
1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院全军肿瘤研究所
2.130062 长春，中国人民解放军第九六四医院放射科
3.100088 北京，中国人民解放军火箭军特色医学中心肿瘤科
- 作者简介：
[查皓然，E-mail: zhahaoran@hotmail.comZHA Haoran, E-mail: zhahaoran@hotmail.com
[朱波，E-mail: bo.zhu@tmmu.edu.cnZHU Bo, E-mail: bo.zhu@tmmu.edu.cn
- 基金信息：国家自然科学基金面上项目(82272808)
DOI：10.16016/j.2097-0927.202402058
中图分类号： R373.1;R392.12;R556.9收稿：2024-02-21，
修回：2024-03-08，
纸质出版：2024-05-15
- 稿件说明：
移动端阅览
杨学智, 侯俊雷, 朱恩健, 等. 肿瘤相关性贫血与SARS-CoV-2特异性记忆CD8+T细胞丰度的相关性分析[J]. 陆军军医大学学报, 2024,46(9):1047-1056.
Xuezhi YANG, Junlei HOU, Enjian ZHU, et al. Correlation between cancer related anemia and abundance of SARS-CoV-2 specific memory CD8+ T cells[J]. Journal of Army Medical University, 2024, 46(9): 1047-1056.
杨学智, 侯俊雷, 朱恩健, 等. 肿瘤相关性贫血与SARS-CoV-2特异性记忆CD8+T细胞丰度的相关性分析[J]. 陆军军医大学学报, 2024,46(9):1047-1056. DOI： 10.16016/j.2097-0927.202402058.
Xuezhi YANG, Junlei HOU, Enjian ZHU, et al. Correlation between cancer related anemia and abundance of SARS-CoV-2 specific memory CD8+ T cells[J]. Journal of Army Medical University, 2024, 46(9): 1047-1056. DOI： 10.16016/j.2097-0927.202402058.
摘要
目的
2
探讨肿瘤相关性贫血对外周血中新型冠状病毒特异性记忆CD8
+
T细胞丰度的影响。
方法
2
纳入2023年6-7月在火箭军特色医学中心住院治疗的HLA-A*11∶01阳性中晚期实体瘤患者34例。根据血红蛋白浓度将患者分为无贫血组(HGB≥120 g/L，
n
=14)、轻度贫血组(120 g/L
>
HGB≥90 g/L，
n
=14)与中重度贫血组(HGB
<
90 g/L，
n
=6)。分离外周血单个核细胞，利用新冠病毒抗原表位刺激并扩增记忆CD8
+
T细胞，使用ELISPOT筛选阳性表位，构建Tetramer。利用扩增后细胞检测Tetramer
+
细胞比例、数量及ELISPOT斑点数，用以反映新冠病毒特异性记忆CD8
+
T细胞丰度。分析患者血红蛋白浓度与Tetramer
+
细胞比例、数量及ELISPOT斑点数的相关性。分析不同贫血组Tetramer
+
细胞比例、数量及ELISPOT斑点数的差异。
结果
2
34例患者中男性25例，女性9例；年龄36~78岁，中位年龄57岁。患者扩增后Tetramer
+
细胞比例、数量及ELISPOT斑点数与血红蛋白浓度均呈正相关关系(
P
＜0.05)；与无贫血组相比，中重度贫血组患者Tetramer
+
细胞比例、数量及ELISPOT斑点数均明显降低(
P
＜0.05)。
结论
2
肿瘤患者外周血新冠病毒特异性记忆CD8
+
T细胞的丰度与血红蛋白浓度正相关，提示肿瘤相关性贫血可能通过降低外周血中记忆CD8
+
T细胞的丰度导致患者病毒易感性增高。
Abstract
Objective
2
To explore the effect of cancer related anemia on the abundance of SARS-CoV-2 specific memory CD8
+
T cells in peripheral blood.
Methods
2
A total of 34 HLA-A*11∶01 positive patients with middle- and late-stage tumors admitted in the Rocket Force Medical Center of PLA from June to July 2023 were enrolled in this study. According to hemoglobin (HGB) level
they were divided into non anemia group (≥120 g/L
n
=14)
mild anemia group (90~120 g/L
n
=14)
and moderate to severe anemia group (
<
90 g/L
n
=6). After their peripheral blood mononuclear cells were isolated
memory CD8
+
T cells were stimulated by the epitopes of SARS-CoV-2 and then expanded. Positive epitopes were screened using enzyme-linked immunosorbent spot (ELISPOT) assay
and then MHC-peptide Tetramers were constructed. The proportion and number of Tetramer
+
cells and the number of ELISPOT spots were detected in the expanded cells to reflect the abundance of SARS-CoV-2 specific memory CD8
+
T cells. The relevance of patients' HGB level with the proportion and number of Tetramer
+
cells and the number of ELISPOT spots was analyzed. The differences of the proportion and number of Tetramer
+
cells and the number of ELISPOT spots was analyzed in different groups.
Results
2
Among the 34 patients
there were 25 males and 9 females
at an age range of 36~78 (median 57) years. The proportion and number of Tetramer
+
cells and the number of ELISPOT spots were positively correlated with HGB level (
P
<
0.05). The proportion and number of Tetramer
+
cells and the number of ELISPOT spots were significantly decreased in patients with moderate to severe anemia than the non anemia patients (
P
<
0.05).
Conclusion
2
The abundance of SARS-CoV-2 specific memory CD8
+
T cells in peripheral blood is positively correlated with patients' HGB level
indicating that CRA may increase the susceptibility of patients to viruses by reducing the abundance of memory CD8
+
T cells in peripheral blood.
关键词
Keywords
references
KNIGHT K, WADE S, BALDUCCI L. Prevalence and outcomes of anemia in cancer: a systematic review of the literature[J]. Am J Med, 2004, 116(Suppl 7A): 11S-26S. DOI: 10.1016/j.amjmed.2003.12.008.
ABDEL-RAZEQ H, HASHEM H. Recent update in the pathogenesis and treatment of chemotherapy and cancer induced anemia[J]. Crit Rev Oncol Hematol, 2020, 145: 102837. DOI: 10.1016/j.critrevonc.2019.102837.
AGARWAL N, AZAD A A, CARLES J, et al . Talazoparib plus enzalutamide in men with first-line metastatic castration-resistant prostate cancer (TALAPRO-2): a randomised, placebo-controlled, phase 3 trial[J ] . Lancet, 2023, 402(10398): 291-303. DOI: 10.1016/S0140-6736(23)01055-3.
ZHAO L T, HE R, LONG H X, et al . Late-stage tumors induce anemia and immunosuppressive extramedullary erythroid progenitor cells[J ] . Nat Med, 2018, 24(10): 1536-1544. DOI: 10.1038/s41591-018-0205-5.
ZHANG L, ZHU F, XIE L, et al . Clinical characteristics of COVID-19-infected cancer patients: a retrospective case study in three hospitals within Wuhan, China[J ] . Ann Oncol, 2020, 31(7): 894-901. DOI: 10.1016/j.annonc.2020.03.296.
HIRSCH H H, MARTINO R, WARD K N, et al . Fourth European Conference on Infections in Leukaemia (ECIL-4): guidelines for diagnosis and treatment of human respiratory syncytial virus, parainfluenza virus, metapneumovirus, rhinovirus, and coronavirus[J ] . Clin Infect Dis, 2013, 56(2): 258-266. DOI: 10.1093/cid/cis844.
TAYYAR R, HO D. Herpes simplex virus and Varicella zoster virus infections in cancer patients[J ] . Viruses, 2023, 15(2): 439. DOI: 10.3390/v15020439.
LEE J, KIM S C, RHEE C K, et al . Prevalence and clinical course of upper airway respiratory virus infection in critically ill patients with hematologic malignancies[J ] . PLoS One, 2021, 16(12): e0260741. DOI: 10.1371/journal.pone.0260741.
SRIDHA R S, BEGOM S, BERMINGHAM A, et al . Cellular immune correlates of protection against symptomatic pandemic influenza[J ] . Nat Med, 2013, 19(10): 1305-1312. DOI: 10.1038/nm.3350.
HASHIMOTO M, KAMPHORST A O, IM S J, et al . CD8 T cell exhaustion in chronic infection and cancer: opportunities for interventions[J ] . Annu Rev Med, 2018, 69: 301-318. DOI: 10.1146/annurev-med-012017-043208.
WILLIAMS M A. Instant recall: a key role for effector-phenotype CD8 + memory T cells in immune protection[J ] . Immunity, 2013, 38(6): 1090-1091. DOI: 10.1016/j.immuni.2013.06.007.
JOSHI N S, CUI W G, CHANDELE A, et al . Inflammation directs memory precursor and short-lived effector CD8 + T cell fates via the graded expression of T-bet transcription factor[J ] . Immunity, 2007, 27(2): 281-295. DOI: 10.1016/j.immuni.2007.07.010..
MCELHANEY J E, XIE D X, HAGER W D, et al . T cell responses are better correlates of vaccine protection in the elderly[J ] . J Immunol, 2006, 176(10): 6333-6339. DOI: 10.4049/jimmunol.176.10.6333.
MARINGER Y, NELDE A, SCHROEDER S M, et al . Durable spike-specific T cell responses after different COVID-19 vaccination regimens are not further e nhanced by booster vaccination[J ] . Sci Immunol, 2022, 7(78): eadd3899. DOI: 10.1126/sciimmunol.add3899.
CATTANEO C M, DIJKSTRA K K, FANCHI L F, et al . Tumor organoid-T-cell coculture systems[J ] . Nat Protoc, 2020, 15(1): 15-39. DOI: 10.1038/s41596-019-0232-9.
EBERHARDT C S, KISSICK H T, PATEL M R, et al . Functional HPV-specific PD-1 + stem-like CD8 T cells in head and neck cancer[J ] . Nature, 2021, 597(7875): 279-284. DOI: 10.1038/s41586-021-03862-z..
SOERENS A G, KVNZLI M, QUARNSTROM C F, et al . Functional T cells are capable of supernumerary cell division and longevity[J ] . Nature, 2023, 614(7949): 762-766. DOI: 10.1038/s41586-022-05626-9.
0
浏览量
0
下载量
0
CSCD
- 网络PDF下载
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备09064830号-19
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
