尼达尼布纳米脂质体的制备及其表征研究

饶乐; 郭梦雨; 徐国纲

doi:10.16016/j.2097-0927.202402025

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尼达尼布纳米脂质体的制备及其表征研究

药学 | 更新时间：2026-01-09

- 尼达尼布纳米脂质体的制备及其表征研究
- Preparation and characterization of nintedanib nano-liposomes
- 陆军军医大学学报 2024年46卷第14期页码：1695-封三
- 作者机构：
  
  1.100853 北京，解放军总医院第二医学中心健康医学科
  2.100190 北京，国家纳米科学中心，中国科学院纳米生物效应与安全性重点实验室
- 作者简介：
  
  [徐国纲，E-mail: gxu@301hospital.com.cnXU Guogang, E-mail: gxu@301hospital.com.cn
- 基金信息：
  
  国家自然科学基金面上项目(82341089);国家自然科学基金面上项目(82370083);国家自然科学基金青年项目(82200091)
- DOI：10.16016/j.2097-0927.202402025
  中图分类号： R944;R961;R977.3
- 收稿：2024-02-05，
  
  修回：2024-03-14，
  
  纸质出版：2024-07-30
- 稿件说明：
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饶乐, 郭梦雨, 徐国纲. 尼达尼布纳米脂质体的制备及其表征研究[J]. 陆军军医大学学报, 2024,46(14):1695-封三.

Le RAO, Mengyu GUO, Guogang XU. Preparation and characterization of nintedanib nano-liposomes[J]. Journal of Army Medical University, 2024, 46(14): 1695-封三.
饶乐, 郭梦雨, 徐国纲. 尼达尼布纳米脂质体的制备及其表征研究[J]. 陆军军医大学学报, 2024,46(14):1695-封三. DOI： 10.16016/j.2097-0927.202402025.

Le RAO, Mengyu GUO, Guogang XU. Preparation and characterization of nintedanib nano-liposomes[J]. Journal of Army Medical University, 2024, 46(14): 1695-封三. DOI： 10.16016/j.2097-0927.202402025.

摘要

目的

探讨4种不同方法制备尼达尼布脂质体的关键理化特性，筛选出尼达尼布脂质体的最佳制备方法。

方法

采用薄膜分散法、逆向蒸发法、乙醇注入法和改良版乙醇注入法制备尼达尼布脂质体；利用透析法测定尼达尼布脂质体的包封率，并进行粒径大小、分散性、表面电位和形貌表征，在室温放置7 d后评估其稳定性。

结果

薄膜分散法、逆向蒸发法、乙醇注入法和改良版乙醇注入法均制备了类圆形尼达尼布脂质体，粒径大小分别为(285.1±12.3)、(271.4±5.9)、(226.3±14.8)、(197.2±11.2)nm；包封率分别为(15.82±1.26)%、(16.71±1.35)%、(25.12±3.21)%和(32.5±3.61)%；与其他3种方法相比，改良版乙醇注入法制备尼达尼布脂质体粒径大小显著减小，包封率显著增加(

＜0.05)。室温放置7 d后，薄膜分散法和逆向蒸发法粒径明显增大(

＜0.01)，乙醇注入法和改良版乙醇注入法粒径大小无明显差异(

0.05)。

结论

改良版乙醇注入法制备的尼达尼布脂质体关键理化特性表现优异，可作为制备尼达尼布脂质体的优选方法。

Abstract

Objective

To investigate the key physical and chemical properties of nintedanib liposomes prepared by 4 different methods in order to screen out the best preparation method of nintedanib liposomes.

Methods

Firstly

nintedanib liposomes were prepared by thin film dispersion

reverse evaporation

ethanol injection and modified ethanol injection

respectively. The entrapment efficiency of nintedanib liposomes was then determined by dialysis and characterized for particle size

dispersibility

surface potential

and morphology. Their stability was evaluated after being placed at room temperature for 7 d.

Results

Circular nintedanib liposomes were respectively prepared by thin film dispersion

reverse evaporation

ethanol injection and modified ethanol injection

with a particle size of (285.1±12.3)

(271.4±5.9)

(226.3±14.8) and (197.2±11.2) nm

an enca

psulation rate of (15.82±1.26)%

(16.71±1.35)%

(25.12±3.21)% and (32.5±3.61)%

respectively. The nintedanib liposomes prepared by modified ethanol injection had significantly smallest particle size and highest encapsulation efficiency than the liposomes by the other 3 methods (

0.05). In 7 d after being placed at room temperature

the particle sizes of the liposomes prepared by film dispersion and reverse evaporation were increased notably (

0.01)

but no such change was observed in the liposomes prepared by ethanol injection and modified ethanol injection (

0.05).

Conclusion

Nintedanib liposomes prepared by modified ethanol injection have excellent key physical and chemical properties

and this method is an optical one for preparing nintedanib liposomes.

关键词

Keywords

references

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