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Del-1纳米颗粒/丝素水凝胶通过促进炎症消退加速慢性皮肤伤口愈合修复
Del-1 nanoparticles/silk fibroin hydrogel accelerates the healing of chronic skin wounds by promoting inflammation regression
- 陆军军医大学学报 2024年46卷第9期 页码：988-996
- 作者机构：
230001 合肥，中国科学技术大学附属第一医院(安徽省立医院)皮肤科
- 作者简介：
[ 唐隽，E-mail: tangjun0212@ustc.edu.cn TANG Jun, E-mail: tangjun0212@ustc.edu.cn
- 基金信息：国家自然科学基金面上项目(82273516)
DOI：10.16016/j.2097-0927.202401069
中图分类号： R364.5;R640.5;R977.6收稿：2024-01-17，
修回：2024-02-29，
纸质出版：2024-05-15
- 稿件说明：
移动端阅览
阚雪薇, 姚萍萍, 陈嘉琪, 等. Del-1纳米颗粒/丝素水凝胶通过促进炎症消退加速慢性皮肤伤口愈合修复[J]. 陆军军医大学学报, 2024,46(9):988-996.
Xuewei KAN, Pingping YAO, Jiaqi CHEN, et al. Del-1 nanoparticles/silk fibroin hydrogel accelerates the healing of chronic skin wounds by promoting inflammation regression[J]. Journal of Army Medical University, 2024, 46(9): 988-996.
阚雪薇, 姚萍萍, 陈嘉琪, 等. Del-1纳米颗粒/丝素水凝胶通过促进炎症消退加速慢性皮肤伤口愈合修复[J]. 陆军军医大学学报, 2024,46(9):988-996. DOI： 10.16016/j.2097-0927.202401069.
Xuewei KAN, Pingping YAO, Jiaqi CHEN, et al. Del-1 nanoparticles/silk fibroin hydrogel accelerates the healing of chronic skin wounds by promoting inflammation regression[J]. Journal of Army Medical University, 2024, 46(9): 988-996. DOI： 10.16016/j.2097-0927.202401069.
摘要
目的
2
探讨负载发育内皮基因座-1(developmental endothelial locus-1，Del-1)纳米颗粒的丝素水凝胶对小鼠慢性皮肤伤口愈合的影响。
方法
2
在6~8周BALB/c小鼠背部皮肤用磁铁挤压12 h放松12 h，循环4 d，形成慢性压疮; 小鼠分为3组，每组8只，分别给予皮肤创面PBS、丝素水凝胶或Del-1纳米颗粒/丝素水凝胶治疗，拍照并计算创面愈合率，连续治疗9 d，HE、Masson染色检测皮肤组织愈合；通过CD14和TNF-α的免疫荧光检测创面巨噬细胞和炎症因子表达；过氧叔丁醇(tert-butyl hydroperoxide，TBHP)体外刺激小鼠巨噬细胞系RAW 264.7和小鼠血管内皮细胞系C166；在C166细胞中过表达Del-1，结晶紫染色检测巨噬细胞迁移；RT-qPCR检测炎症因子IL-6表达。
结果
2
小鼠实验证实Del-1纳米颗粒/丝素水凝胶组的创面愈合快于丝素水凝胶组和PBS组(
P
＜0.01)；Del-1纳米颗粒/丝素水凝胶组创面的TNF-α及CD14的表达低于丝素水凝胶组和PBS组(
P
＜0.01)，但胶原沉积和组织修复高于PBS和丝素水凝胶组(
P
＜0.01)。体外实验证实TBHP激活巨噬细胞向内皮细胞迁移，但Del-1过表达组巨噬细胞迁移率显著下降(
P
＜0.01)；RT-qPCR显示Del-1抑制了IL-6表达(
P
＜0.01)。
结论
2
Del-1纳米颗粒/丝素水凝胶能够加速小鼠压疮愈合，其机制可能是通过促进炎症消退和组织修复。
Abstract
Objective
2
To investigate the effect of silk fibroin hydrogel loaded with developmental endothelial locus-1 (Del-1) nanoparticles on the healing of chronic skin wounds in mice.
Methods
2
The back skin of BALB/c mice (6-8 weeks old) was pressed with a magnet for 12 h and then relaxed for 12 h
for 4 consecutive days to establish a chronic pressure ulcer wound. After infliction
the mice were randomly di
vided into 3 groups (
n
=8)
and the skin wounds were treated with PBS
silk fibroin hydrogel or Del-1 nanoparticles/silk fibroin hydrogel. The wound healing was recorded with camera to calculate the wound healing rate. In 9 d after treatment
HE and Masson staining were used to observe the wound healing
and immunofluorescence staining for CD14 and TNF-α was used to compare the appearance frequency of skin macrophages and the expression of inflammatory factors. After Tert-butyl peroxide (TBHP) was used to stimulate mouse macrophage RAW 264.7 cells and mouse vascular endothelial C166 cells
C166 cells were transfected with lentival vector to overexpress Del-1. Crystal violet staining was used to observe the migration of macrophages. RT-qPCR was used to detect the expression of inflammatory factor IL-6.
Results
2
The wound healing was significantly faster in the Del-1 nanoparticles/silk fibroin hydrogel group than the silk fibroin hydrogel group and the PBS group (
P
<
0.01). The expression levels of TNF-α and CD14 in the wound surface were lower (
P
<
0.01)
but collagen deposition and tissue repair were better in the Del-1 nanoparticles/silk fibroin hydrogel group than the silk fibroin hydrogel group and the PBS group (
P
<
0.01).
In vitro
experiments
macrophages migrated to endothelial cells stimulated by TBHP
but the migration rate of macrophages was significantly lower in the Del-1 overexpression group (
P
<
0.01). RT-qPCR confirmed that Del-1 inhibited the transcription of IL-6 (
P
<
0.01).
Conclusion
2
Del-1 nanoparticles/silk fibroin hydrogel can significantly accelerate the healing of skin wounds
and its mechanism may be through promoting the regression of inflammation and tissue repair.
关键词
Keywords
references
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