雌性和雄性非肥胖糖尿病小鼠中CD8<sup>+</sup> T细胞分化亚群差异研究

杨数数; 刘东; 黎婕; 张梦军; 王莉

doi:10.16016/j.2097-0927.202312058

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雌性和雄性非肥胖糖尿病小鼠中CD8⁺ T细胞分化亚群差异研究

基础医学 | 更新时间：2026-01-09

- 雌性和雄性非肥胖糖尿病小鼠中CD8⁺ T细胞分化亚群差异研究
- Characterization of CD8⁺ T cell subsets in male and female non-obese diabetic mice
- 陆军军医大学学报 2024年46卷第8期页码：837-848
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)：基础医学院免疫学教研室
  2.400038 重庆，陆军军医大学(第三军医大学)：药学与检验医学系药物分析学教研室
- 作者简介：
  
  [王莉, E-mail: liwang118@tmmu.edu.cnWANG Li, E-mail: liwang118@tmmu.edu.cn
- 基金信息：
  
  国家自然科学基金面上项目(82071825);国家自然科学基金面上项目(81871301)
- DOI：10.16016/j.2097-0927.202312058
  中图分类号： R339.2;R392.12;R587.1
- 收稿：2023-12-11，
  
  修回：2024-01-10，
  
  纸质出版：2024-04-30
- 稿件说明：
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杨数数, 刘东, 黎婕, 等. 雌性和雄性非肥胖糖尿病小鼠中CD8⁺ T细胞分化亚群差异研究[J]. 陆军军医大学学报, 2024,46(8):837-848.

Shushu YANG, Dong LIU, Jie LI, et al. Characterization of CD8⁺ T cell subsets in male and female non-obese diabetic mice[J]. Journal of Army Medical University, 2024, 46(8): 837-848.
杨数数, 刘东, 黎婕, 等. 雌性和雄性非肥胖糖尿病小鼠中CD8⁺ T细胞分化亚群差异研究[J]. 陆军军医大学学报, 2024,46(8):837-848. DOI： 10.16016/j.2097-0927.202312058.

Shushu YANG, Dong LIU, Jie LI, et al. Characterization of CD8⁺ T cell subsets in male and female non-obese diabetic mice[J]. Journal of Army Medical University, 2024, 46(8): 837-848. DOI： 10.16016/j.2097-0927.202312058.

摘要

目的

以雌性和雄性非肥胖糖尿病(nonobese diabetic

NOD)小鼠以及健康对照癌症研究所(institute for cancer research

ICR)小鼠为研究对象，比较分析初始、效应、记忆、耗竭以及调节性CD8

T细胞分化亚群差异，探讨1型糖尿病(type 1 diabetes

T1D)背景下的性别因素对CD8

T细胞分化命运的影响。

方法

采用流式细胞术检测雌雄NOD小鼠脾脏、胰腺引流淋巴结(pancreatic draining lymph nodes

pLN)、胰腺浸润淋巴细胞(pancreas-infiltrating lymphocytes

PIL)、初始T细胞(na?ve T cells

)、中枢记忆T细胞(central memory T cells

)、效应T细胞(effector T cells

EFF

)、效应前体样T细胞(effector precursor T cells

)、耗竭T细胞(exhausted T cells

)、耗竭前体T细胞(precursor exhausted T cells

PEX

)以及调节性T细胞(regulatory T cells

Tregs)等CD8

T细胞分化亚群的频率和表型差异。

结果

与雄性NOD小鼠比较，雌性NOD小鼠pLN及PIL中IFN-γ

、CD107a

和CCL5

CD8

EFF

频率显著升高(

0.01

0.05

0.05)，同时脾脏中CD8

、CD8

PEX

和CD122

CD8

Tregs亚群的频率均显著降低(

0.01

0.001)；而雌性和雄性ICR小鼠体内除CD122

CD8

Tregs差异变化与NOD小鼠一致(

0.05)，其余上述各CD8

T细胞分化亚群无显著差异。

结论

雄激素可能通过抑制记忆T细胞向效应T细胞分化，同时促进效应T细胞功能耗竭，导致雌雄发病率差异。

Abstract

Objective

To compare and analyze the differences in CD8

naive

effector

memory

exhausted and regulatory T cells in order to investigate the impact of gender on the differentiation fate of CD8

T cells in the context of type 1 diabetes (T1D) based on female and male non-obese diabetic (NOD) mice and healthy Institute for Cancer Research (ICR) mice.

Methods

The frequencies and phenotypes of CD8

T cell differentiation subsets including naive T cells (T

)

central memory T cells (T

)

effector T cells (T

EFF

)

effector precursor T cells (T

)

exhausted T cells (T

)

precursor exhausted T cells (T

PEX

) and regulatory T cells (Tregs) in the spleen

pancreatic draining lymph nodes (pLN) and pancreas infiltrating lymphocytes (PIL) of male and female NOD mice were detected by flow cytometry.

Results

The frequencies of IFN-γ

CD107a

and CCL5

CD8

EFF

in pLN and PIL of female NOD mice were significantly higher than those of

male NOD mice. However

the frequencies of CD8

CD8

PEX

and CD122

CD8

Tregs subsets in the spleen were significantly decreased. While there were no significant differences in the above CD8

T cell subsets except CD8

Tregs between female and male ICR mice.

Conclusion

Androgen may inhibit the differentiation of memory T cells into effector T cells and promote the exhaustion of effector T cells

leading to the difference in morbidity between the male and female mice.

关键词

Keywords

references

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雌性和雄性非肥胖糖尿病小鼠中CD8+ T细胞分化亚群差异研究

Characterization of CD8+ T cell subsets in male and female non-obese diabetic mice

DOI：10.16016/j.2097-0927.202312058

摘要

Abstract

关键词

Keywords

references

雌性和雄性非肥胖糖尿病小鼠中CD8⁺ T细胞分化亚群差异研究

Characterization of CD8⁺ T cell subsets in male and female non-obese diabetic mice