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内侧前额叶皮质谷氨酸能神经元参与CD1小鼠攻击行为的机制研究
Mechanism of glutaminergic neurons in medial prefrontal cortex involved in aggressive behavior of CD1 mice
- 陆军军医大学学报 2024年46卷第12期 页码：1336-1343
- 作者机构：
1.400038 重庆，陆军军医大学(第三军医大学)：第二附属医院麻醉科
2.400038 重庆，陆军军医大学(第三军医大学)：基础医学院神经生物教研室
- 作者简介：
[李洪，E-mail：lh78553@tmmu.edu.cn LI Hong, E-mail: lh78553@tmmu.edu.cn
[白福海，E-mail：bfh@tmmu.edu.cn BAI Fuhai, E-mail: bfh@tmmu.edu.cn
- 基金信息：国家自然科学基金(82101273);国家自然科学基金面上项目(82171265);中国博士后面上项目(2021M693936);重庆市博士后科学基金项目(cstc2021jcyj-bshX0071);陆军军医大学科技创新能力提升专项(2021XQN19);陆军军医大学第二附属医院青年博士人才孵化计划(2023YQB007);重庆市博士后研究项目特别资助(2022CQBSHTB3021)
DOI：10.16016/j.2097-0927.202312020
中图分类号： R338.12;R338.6;R394.1收稿：2023-12-05，
修回：2024-01-18，
纸质出版：2024-06-30
- 稿件说明：
移动端阅览
李加欣, 黄露, 龙宗泓, 等. 内侧前额叶皮质谷氨酸能神经元参与CD1小鼠攻击行为的机制研究[J]. 陆军军医大学学报, 2024,46(12):1336-1343.
Jiaxin LI, Lu HUANG, Zonghong LONG, et al. Mechanism of glutaminergic neurons in medial prefrontal cortex involved in aggressive behavior of CD1 mice[J]. Journal of Army Medical University, 2024, 46(12): 1336-1343.
李加欣, 黄露, 龙宗泓, 等. 内侧前额叶皮质谷氨酸能神经元参与CD1小鼠攻击行为的机制研究[J]. 陆军军医大学学报, 2024,46(12):1336-1343. DOI： 10.16016/j.2097-0927.202312020.
Jiaxin LI, Lu HUANG, Zonghong LONG, et al. Mechanism of glutaminergic neurons in medial prefrontal cortex involved in aggressive behavior of CD1 mice[J]. Journal of Army Medical University, 2024, 46(12): 1336-1343. DOI： 10.16016/j.2097-0927.202312020.
摘要
目的
2
研究CD1小鼠发动攻击行为的内在神经机制。
方法
2
通过驻地入侵实验筛选出具有攻击行为的CD1小鼠，攻击配对偏好进一步验证后使用c-Fos标记全脑激活的脑区，通过免疫荧光双标实验分析攻击行为激活了哪种类型的神经元，最后使用光遗传学调控该类神经元的活性，观察其对攻击行为的影响。
结果
2
通过c-Fos筛选，约82%的CD1小鼠表现出攻击行为；攻击行为发生后主要激活内侧前额叶皮质(medial prefrontal cortex
mPFC)，免疫荧光双标结果显示mPFC脑区c-Fos阳性神经元主要是谷氨酸能神经元；最后，我们通过光遗传激活mPFC脑区谷氨酸能神经元，发现其能够抑制CD1小鼠的攻击行为；相反，光遗传抑制mPFC脑区谷氨酸能神经元，能够促进CD1小鼠的攻击行为。
结论
2
mPFC脑区谷氨酸能神经元是调控CD1小鼠发动攻击行为的重要组成部分。
Abstract
Objective
2
To investigate the intrinsic neural mechanism of aggressive behavior in CD1 mice.
Methods
2
CD1 mice with aggressive behavior were screened out by resident intruder test. After the aggressive conditioned pair preference was further verified
the activated brain regions of the whole brain were labeled with c-Fos
and the types of neurons activated by the aggressive behavior were analyzed by double immunofluorescence labeling. Finally
the effects of activity of these neurons regulated by optogenetics on aggressive behavior were observed.
Results
2
The c-Fos screening revealed that about 82% of the CD1 mice showed aggressive behavior. After the occurrence of aggressive behavior
the main activation occured in the medial prefrontal cortex (mPFC)
and the results of immunofluorescence double labeling showed that the c-Fos positive neurons in the mPFC were mainly glutamatergic neurons. Finally
glutamatergic neurons in the mPFC could be activated by optogenetics
and the activation inhibited the aggressive behavior of CD1 mice. In contrast
optogenetics could inhibit glutamatergic neurons in the mPFC and then promote the aggressive behavior of CD1 mice.
Conclusion
2
Glutamatergic neurons in the mPFC are an important component in the regulation of aggressive behavior in CD1 mice.
关键词
Keywords
references
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