NSAIDs对钛片表面成骨细胞增殖及功能活性的影响

谢孟; 莫安春

doi:10.16016/j.2097-0927.202311078

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NSAIDs对钛片表面成骨细胞增殖及功能活性的影响

基础医学 | 更新时间：2026-01-09

- NSAIDs对钛片表面成骨细胞增殖及功能活性的影响
- Effect of non-steroidal anti-inflammatory drugs on growth and functional activity of osteoblasts in vitro
- 陆军军医大学学报 2024年46卷第15期页码：1772-1779
- 作者机构：
  
  1.610044 成都，四川口腔医院种植修复科
  2.610041 成都，口腔疾病研究国家重点实验室，国家口腔疾病临床医学研究中心，四川大学华西口腔医院种植科
- 作者简介：
  
  [莫安春，E-mail: moanchun@163.comMO Anchun, E-mail: moanchun@163.com
- 基金信息：
- DOI：10.16016/j.2097-0927.202311078
  中图分类号： R322.71;R783.1;R971.1
- 收稿：2023-11-16，
  
  修回：2023-12-11，
  
  纸质出版：2024-08-15
- 稿件说明：
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谢孟, 莫安春. NSAIDs对钛片表面成骨细胞增殖及功能活性的影响[J]. 陆军军医大学学报, 2024,46(15):1772-1779.

Meng XIE, Anchun MO. Effect of non-steroidal anti-inflammatory drugs on growth and functional activity of osteoblasts in vitro[J]. Journal of Army Medical University, 2024, 46(15): 1772-1779.
谢孟, 莫安春. NSAIDs对钛片表面成骨细胞增殖及功能活性的影响[J]. 陆军军医大学学报, 2024,46(15):1772-1779. DOI： 10.16016/j.2097-0927.202311078.

Meng XIE, Anchun MO. Effect of non-steroidal anti-inflammatory drugs on growth and functional activity of osteoblasts in vitro[J]. Journal of Army Medical University, 2024, 46(15): 1772-1779. DOI： 10.16016/j.2097-0927.202311078.

摘要

目的

通过建立种植体表面成骨模型，研究新型与传统非甾体类抗炎药(nonsteroidal antiinflammatory drugs

NSAIDs)对钛片表面MG-63成骨细胞增殖、形态、粘附、活性和成骨相关基因表达的影响。

方法

将MG-63成骨细胞接种至大颗粒喷砂酸蚀(sand-blasted

largegrit

acid-etched

SLA)钛片表面，建立种植体表面与成骨细胞结合的模型。设置一氧化氮供体型氟比洛芬(NO-氟比洛芬)组、氟比洛芬组与无药物对照组。CCK-8检测细胞增殖能力，扫描电镜观察细胞形态，MTT检测细胞粘附能力，化学法检测碱性磷酸酶(ALP)活性，茜素红染色观察钙化结节，RT-qPCR检测各组成骨细胞ALP、OCN、Runx-2 mRNA表达情况。

结果

细胞增殖检测结果显示，不同药物浓度组间的细胞增殖数量有统计学差异(

0.01)；NO-氟比洛芬组和氟比洛芬组细胞增殖数量均高于无药物对照组，且NO-氟比洛芬组高于氟比洛芬组，差异均有统计学意义(

0.01)。扫描电镜观察结果显示，NO-氟比洛芬组成骨细胞最先开始复层生长。细胞粘附检测结果显示，NO-氟比洛芬组细胞粘附数量低于氟比洛芬组和无药物对照组(

0.01)。ALP活性检测结果显示，NO-氟比洛芬组和氟比洛芬组ALP活性低于无药物对照组(

0.01)。茜素红染色结果显示，NO-氟比洛芬组及氟比洛芬组实验观察期内未观察到典型的红色深染钙化结节。RT-qPCR分析显示，NO-氟比洛芬组OCN mRNA和氟比洛芬组Runx-2 mRNA表达量高于无药物对照组，ALP mRNA表达量低于无药物对照组(

0.01)。

结论

NO-氟比洛芬和氟比洛芬均有效促进钛片表面成骨细胞的增殖能力和部分成骨相关基因表达，NO-氟比洛芬的促进效果更为明显。

Abstract

Objective

To investigate the effects of new and traditional non-steroidal anti-inflammatory drugs (NSAIDs) on the proliferation

morphology

adhesion

activity and expression of osteoblast related genes of MG-63 osteoblasts by establishing a osteogenic model of implants.

Methods

MG-63 cells were implanted on sand-blasted

largegrit

acid-etched titanium plates surface (SLA) to establish a model of osseointegration between implant surface and osteoblasts. The experiments included NO-flurbiprofen group

flurbiprofen group and the control group. Cell proliferation was detected by CCK-8 assay

cell morphology was observed by scanning electron microscopy (SEM)

cell adhesion was detected by MTT assay

alkaline phosphatase (ALP) activity was detected by chemical method

calcified nodules was observed after Alizarin red staining

and the expression of bone formation related genes ALP

OCN

Runx-2 was detected by RT-qPCR.

Results

CCK-8 assay showed that statistical diffe

rence was observed in cell proliferation among the groups treated with different drug concentrations (

0.01). Cell proliferation was higher in the NO-flurbiprofen group and the flurbiprofen group than the control group

and in the NO-flurbiprofen group than the flurbiprofen group (

0.01). SEM displayed that the cells from the NO-flurbiprofen group grew to multiple lays first. Cell adhesion results showed that the number of cell adhesion was lower in the NO-flurbiprofen group than the flurbiprofen group and the control group (

0.01). ALP activity of drug groups was lower than the control group (

0.01). No typical red deeply stained calcified nodules were observed in neither NO-flurbiprofen group nor flurbiprofen group during the observation period. RT-qPCR indicated that the mRNA expression of OCN in the NO-flurbiprofen group and Runx-2 in the flurbiprofen group was higher than those of the control group

while the mRNA level of ALP was lower than that of the control group (

0.01).

Conclusion

Both NO-flurbiprofen and flurbiprofen effectively promote the proliferation of osteoblasts on the surface of titanium plates and the expression of some osteogenic related genes. The promotion effect of NO-flurbiprofen is more significant.

关键词

Keywords

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