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氧糖剥离预处理通过保护线粒体减轻PC12细胞氧糖剥离/再灌注损伤
Oxygen-glucose deprivation preconditioning alleviates oxygen-glucose deprivation/reperfusion injury in PC12 cells by protecting mitochondria
- 陆军军医大学学报 2024年46卷第15期 页码：1763-1771
- 作者机构：
1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院麻醉科
2.646000 四川 泸州，西南医科大学基础医学院解剖教研室
- 作者简介：
[杨朝鲜, E-mail：lyycx@foxmail.comYANG Chaoxian, E-mail: lyycx@foxmail.com
- 基金信息：四川省自然科学基金(2022NSFSC0718);西南医科大学应用基础面上项目(2021ZKMS011)
DOI：10.16016/j.2097-0927.202311019
中图分类号： R329.25;R363.22;R741.02收稿：2023-11-05，
修回：2023-12-06，
纸质出版：2024-08-15
- 稿件说明：
移动端阅览
黄丽, 许娜, 杨朝鲜. 氧糖剥离预处理通过保护线粒体减轻PC12细胞氧糖剥离/再灌注损伤[J]. 陆军军医大学学报, 2024,46(15):1763-1771.
Li HUANG, Na XU, Chaoxian YANG. Oxygen-glucose deprivation preconditioning alleviates oxygen-glucose deprivation/reperfusion injury in PC12 cells by protecting mitochondria[J]. Journal of Army Medical University, 2024, 46(15): 1763-1771.
黄丽, 许娜, 杨朝鲜. 氧糖剥离预处理通过保护线粒体减轻PC12细胞氧糖剥离/再灌注损伤[J]. 陆军军医大学学报, 2024,46(15):1763-1771. DOI： 10.16016/j.2097-0927.202311019.
Li HUANG, Na XU, Chaoxian YANG. Oxygen-glucose deprivation preconditioning alleviates oxygen-glucose deprivation/reperfusion injury in PC12 cells by protecting mitochondria[J]. Journal of Army Medical University, 2024, 46(15): 1763-1771. DOI： 10.16016/j.2097-0927.202311019.
摘要
目的
2
研究氧糖剥离预处理(oxygen-glucose deprivation preconditioning，OGDPC)对氧糖剥离/再灌注(oxygen-glucose deprivation/reperfusion
OGD/R)损伤PC12细胞的保护作用及机制。
方法
2
简单随机抽样将PC12细胞分为Control、OGDPC、OGD/R和OGDPC+OGD/R共4组。透射电镜观察线粒体的超微结构，Mito-tracker染色观察线粒体数量，Western blot检测OGDH、PDHA1、COX4、HSP60的表达，JC-1和ATP试剂盒评估线粒体膜电位及ATP水平，TUNEL染色观察细胞凋亡情况，并与AIF、EndoG的免疫荧光染色进行共定位。
结果
2
与OGD/R组相比，OGDPC+OGD/R组PC12细胞线粒体超微结构损伤较轻；OGDH、PDHA1和COX4的表达增多(
P
<
0.05)，HSP60的表达减少(
P
<
0.05)；线粒体膜电位及ATP水平提高(
P
<
0.05)，细胞凋亡率降低(
P
<
0.05)，且AIF、EndoG向TUNEL染色阳性的细胞核聚集减少(
P
<
0.05)。
结论
2
OGDPC减轻OGD/R引起的PC12细胞损伤，其机制可能与改善线粒体结构和功能、减少线粒体途径引起的细胞凋亡有关。
Abstract
Objective
2
To investigate the protective effect of oxygen-glucose deprivation preconditioning (OGDPC) on PC12 cells damaged with oxygen-glucose deprivation/reperfusion (OGD/R) and its underlying mechanism.
Methods
2
PC12 cells were randomly divided into Control
OGDPC
OGD/R and OGDPC+OGD/R groups. The morphology and quantity of mitochondria were observed by transmission electron microscopy (TEM) and Mito-tracker staining. The expression of OGDH
PDHA1
COX4 and HSP60 was detected by Western blotting. Mitochondrial membrane potential and ATP production were evaluated by JC-1 and ATP assay kit. TUNEL staining was used to assess cell apoptosis
and translocation of AIF and EnodoG was observed by immunofluorescence staining and TUNEL staining.
Results
2
Compared with the OGD/R group
the mitochondrial ultrast
ructure damage were alleviated
the expression levels of OGDH
PDHA1 and COX4 were enhanced (
P
<
0.05)
that of HSP60 was decreased (
P
<
0.05)
the mitochondrial membrane potential and ATP level were increased (
P
<
0.05)
the cell apoptotic rate was reduced (
P
<
0.05)
and AIF and EndoG were less aggregated to the TUNEL-positive nucleus in OGDPC+OGD/R group (
P
<
0.05).
Conclusion
2
OGDPC can alleviate the injury of PC12 cells caused by OGD/R
and the potential mechanism may be related to improving mitochondrial structure and function
and reducing cell apoptosis
via
mitochondrial pathway.
关键词
Keywords
references
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