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Ms 4a 6d 基因敲除对雌性小鼠生育力的影响Effects of
Ms 4a 6d knockout on fertility in female mice- 陆军军医大学学报 2024年46卷第10期 页码：1115-1122
- 作者机构：
400038 重庆，陆军军医大学(第三军医大学)第一附属医院生殖医学中心
- 作者简介：
[何畏，E-mail: anyhewei@163.com HE Wei, E-mail: anyhewei@163.com
- 基金信息：重庆市自然科学基金面上项目(CSTB2022NSCQ-MSX0155);国家自然科学基金面上项目(81873860)
DOI：10.16016/j.2097-0927.202310113
中图分类号： R339.22;R394-33;R394.1收稿：2023-10-31，
修回：2024-03-10，
纸质出版：2024-05-30
- 稿件说明：
移动端阅览
亢恩川, 王莹, 曾继涛, 等.
Ms 4a 6d 基因敲除对雌性小鼠生育力的影响[J]. 陆军军医大学学报, 2024,46(10):1115-1122.Enchuan KANG, Ying WANG, Jitao ZENG, et al. Effects of
Ms 4a 6d knockout on fertility in female mice[J]. Journal of Army Medical University, 2024, 46(10): 1115-1122.亢恩川, 王莹, 曾继涛, 等.
Ms 4a 6d 基因敲除对雌性小鼠生育力的影响[J]. 陆军军医大学学报, 2024,46(10):1115-1122. DOI： 10.16016/j.2097-0927.202310113.Enchuan KANG, Ying WANG, Jitao ZENG, et al. Effects of
Ms 4a 6d knockout on fertility in female mice[J]. Journal of Army Medical University, 2024, 46(10): 1115-1122. DOI： 10.16016/j.2097-0927.202310113.
摘要
目的
2
探究巨噬细胞表面特异分子跨膜4域亚家族A成员6D(membrane-spanning 4-domains subfamily A member 6D，
Ms
4
a
6
d
)基因敲除对雌性小鼠生育力的影响。
方法
2
构建
Ms
4
a
6
d
基因敲除小鼠，以各周龄纯合
Ms
4
a
6
d
基因敲除(
Ms
4
a
6
d
-/-
)小鼠为实验组；采用qPCR、琼脂糖凝胶法鉴定小鼠基因型；运用HE染色、免疫荧光、ELISA等方法检测血清抗缪勒管激素(anti-müllerian hormone，AMH)和雌性
Ms
4
a
6
d
-/-
小鼠卵巢中巨噬细胞数量及各级卵泡构成变化；通过生育力实验比较成年
Ms
4
a
6
d
-/-
雌鼠妊娠率及平均产仔数变化。
结果
2
与同周龄野生型(
Ms
4
a
6
d
+/+
)雌鼠比较，2周龄和4周龄的
Ms
4
a
6
d
-/-
雌鼠卵巢组织中巨噬细胞显著减少(
P
<
0.01)；8周龄时2组卵巢巨噬细胞差异无统计学意义；8周龄
Ms
4
a
6
d
-/-
雌鼠卵巢系数显著降低(
P
<
0.01)；8周龄时卵巢组织中原始卵泡、初级卵泡、次级卵泡和窦卵泡数量显著减少(
P
<
0.05)，各年龄段
Ms
4
a
6
d
-/-
雌鼠血清中AMH显著降低(
P
<
0.05)。成年
Ms
4
a
6
d
-/-
雌鼠的平均妊娠率为56%，每胎产仔数为(4.1±1.1)只，均显著低于同龄野生型雌鼠的妊娠率(89%)和每胎产仔数[(6.3±1.2)只
]
。
结论
2
Ms
4
a
6
d
基因敲除导致青春期前雌鼠卵巢组织中巨噬细胞数量减少，抑制其生长卵泡发育，最终表现为成年雌鼠卵巢储备减少，生育力降低。
Abstract
Objective
2
To investigate the effect of membrane-spanning 4-domains subfamily member 6D (MS4A6D) gene knockout on the fertility of female mice.
Methods
2
On the basis of the successful construction of Ms4a6d gene knockout model mice
the homozygous Ms4a6d gene knockout (
Ms
4
a
6
d
-/-
) mice at an age of different weeks were subjected. Mouse genotype was identified with RT-qPCR and agarose gel electrophoresis. HE staining
immunofluorescence assay and ELISA were used to detect serum level of anti-müllerian hormone (AMH)
composition of macrophages and follicles at all levels in the ovaries of
Ms
4
a
6
d
-/-
mice
respectively. The fertility changes in pregnant rate and average litter size of female
Ms
4
a
6
d
-/-
mice were observed by fertility experiments.
Results
2
Compared with the wild-type (
Ms
4
a
6
d
+/+
) female mice at the same age
there were less macrophages in the ovarian tissues of the 2- and 4-week-old
Ms
4
a
6
d
-/-
female mice (
P
<
0.01)
but no significant difference was observed between the 2 types of mice at 8 weeks of age. For the adult
Ms
4
a
6
d
-/-
female mice at an age of 8 weeks
the ovarian coefficient was obviously lower (
P
<
0.01)
and the numbers of primordial
primary
secondary and antral follicles in the ovarian tissues was statistically lower (
P
<
0.05) when compared with the adult wild-type female mice a
t the same age. The serum AMH level was notably lower in the
Ms
4
a
6
d
-/-
female mice of all ages than the wild-type ones at corresponding ages (
P
<
0.05). The average pregnancy rate was 56% and the litter size was 4.1±1.1 in adult
Ms
4
a
6
d
-/-
female mice
which were significantly lower than those of wild-type mice of the same age (89% and 6.3±1.2).
Conclusion
2
Ms4a6d gene knockout decreases the number of macrophages in the ovarian tissues of adolescent females
interferes with the development of follicles in the ovarian tissues of adolescent females
and leads to a decrease in ovarian reserve in adult females and a decrease in fertility in adult females.
关键词
Keywords
references
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