等能量高脂饮食对SD大鼠能量代谢及耐力运动能力的影响

陈帅; 邓项元; 郎和东; 张瑞良; 饶昕; 袁家奇; 朱俊东; 秦玉; 糜漫天

doi:10.16016/j.2097-0927.202310021

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等能量高脂饮食对SD大鼠能量代谢及耐力运动能力的影响

公共卫生与预防医学 | 更新时间：2026-01-09

- 等能量高脂饮食对SD大鼠能量代谢及耐力运动能力的影响
- Effects of isocaloric high-fat diet on energy metabolism and endurance exercise capacity in SD rats
- 陆军军医大学学报 2024年46卷第9期页码：940-951
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系营养与食品安全研究中心，重庆市医学营养研究中心，营养与健康重庆市重点实验室
- 作者简介：
  
  [秦玉, Email: yuer_forever@163.comQIN Yu, Email: yuer_forever@163.com
  [糜漫天, Email: mantian_mi@tmmu.edu.cnMI Mantian, Email: mantian_mi@tmmu.edu.cn
- 基金信息：
- DOI：10.16016/j.2097-0927.202310021
  中图分类号： R151.1;R151.41;R153.4
- 收稿：2023-10-07，
  
  修回：2024-01-08，
  
  纸质出版：2024-05-15
- 稿件说明：
移动端阅览
陈帅, 邓项元, 郎和东, 等. 等能量高脂饮食对SD大鼠能量代谢及耐力运动能力的影响[J]. 陆军军医大学学报, 2024,46(9):940-951.

Shuai CHEN, Xiangyuan DENG, Hedong LANG, et al. Effects of isocaloric high-fat diet on energy metabolism and endurance exercise capacity in SD rats[J]. Journal of Army Medical University, 2024, 46(9): 940-951.
陈帅, 邓项元, 郎和东, 等. 等能量高脂饮食对SD大鼠能量代谢及耐力运动能力的影响[J]. 陆军军医大学学报, 2024,46(9):940-951. DOI： 10.16016/j.2097-0927.202310021.

Shuai CHEN, Xiangyuan DENG, Hedong LANG, et al. Effects of isocaloric high-fat diet on energy metabolism and endurance exercise capacity in SD rats[J]. Journal of Army Medical University, 2024, 46(9): 940-951. DOI： 10.16016/j.2097-0927.202310021.

摘要

目的

探讨等能量的45%高脂饮食对SD大鼠代谢及耐力运动能力的影响。

方法

24只雄性SD大鼠采用随机数字表法分为：常规喂养组(CON组)、高脂喂养组(HFD组)、常规喂养+训练组(CONT组)、高脂喂养+训练组(HFDT组)。其中CON组、CONT组给予常规饲料喂养，HFD组、HFDT组给予等能量的45%高脂饲料喂养；HFDT组和CONT组同时进行中等强度跑步耐力训练，持续6周。每周测量体质量、脂肪体质量和瘦体质量。用Pheno Master/Calo Treadmill系统测量静息和运动状态下的能量消耗和基础代谢率。在大鼠进行力竭测试后，检测血糖、血脂、血清肌酸激酶等指标。

结果

干预6周后，HFDT组耐力运动能力较CONT组增强(

0.05)。等能量喂养的各组大鼠体质量和体成分没有明显差异。静息时，各组大鼠总能量消耗与基础代谢率无明显差异，但在4周以前，CON组以碳水化合物为主要能量底物，HFD组则以脂肪为主，而碳水代谢率降低后升高，最终两组底物代谢率于第5~6周时达到相似水平。HFDT组以脂肪为主要代谢底物，CONT组以碳水化合物为主要代谢底物，在训练6周后仍存在统计学差异(

0.05)。高脂饮食升高了HFD组大鼠血清胆固醇、TG、HDL-C等指标水平，但经历耐力训练的高脂饮食大鼠(HFDT组)血脂水平有所降低，且HFDT组β羟基丁酸(βHB)水平升高(

0.05)。等能量不同饮食对肝肾功能及肌肉损伤指标的影响没有明显差别。

结论

等能量高脂饮食可通过提高大鼠脂肪利用的能力，从而延长耐力运动时间，而不改变体成分或影响肝肾功能。

Abstract

Objective

To investigate the effects of a 45% high-fat diet (HFD) with isocaloric intake on energy metabolism and endurance exercise capacity in SD rats.

Methods

Twenty-four male SD rats were randomly divided into normal chow diet group (CON)

HFD group

normal chow diet+exercise training group (CONT)

and HFD+exercise training group (HFDT). The CON and CONT groups received normal chow diet

while the HFD and HFDT groups received a 45% high-fat diet with isocaloric intake. The HFDT and CONT groups underwent an endurance training of moderate-intensity running for 6 weeks. Body weight

fat mass

and lean mass were measured weekly. Energy expenditure and basal metabolic rate during rest and exercise states were measured using Pheno Master/Calo Treadmill system. Blood glucose

lipids

and creatine kinase levels were detected after the exhaustion test.

Results

In 6 weeks after intervention

the endurance exercise capacity was significantly enhanced in the HFDT group than the CONT group (

0.05). There were no obvious differences in body weight and body composition among the groups under isoenergetic feeding conditions. At rest

no statistical differences were observed in total energy expenditure and basal metabolic rate among the groups. However

prior to the 4th week

the CON group primarily metabolized carbohydrates while the HFD group primarily metabolized fats. But the carbohydrate metabolism was decreased and then increased

and the substrate metabolism rates eventually reached similar levels between the 2 groups on the 5th to 6th week. The HFDT group primarily metabolized fats while the CONT group primarily metabolized carbohydrates

with significant differences persisting after 6 weeks of training (

0.05). HFD led to elevated levels of serum cholesterol

triglycerides (TG)

and high-density lipoprotein cholesterol (HDL-C)

but

endurance training resulted in decreased lipid levels in the HFDT group

accompanied by an increase in β-hydroxybutyrate (βHB) level (

0.05). Isoenergetic diets had no significant differences in their effects on liver and kidney function or muscle damage indicators.

Conclusion

An isoenergetic HFD can improve fat utilization ability and extend endurance exercise time in rats without altering body composition or affecting liver and kidney function.

关键词

Keywords

references

PURDOM T, KRAVITZ L, DOKLADNY K, et al . Understanding the factors that effect maximal fat oxidation[J ] . J Int Soc Sports Nutr, 2018, 15: 3. DOI: 10.1186/s12970-018-0207-1.

WALDMAN H S, KRINGS B M, BASHAM S A, et al . Effects of a 15-day low carbohydrate, high-fat diet in resistance-trained men[J ] . J Strength Cond Res, 2018, 32(11): 3103-3111. DOI: 10.1519/JSC.0000000000002282.

BURKE L M, WHITFIELD J, HEIKURA I A, et al . Adaptation to a low carbohydrate high fat diet is rapid but impairs endurance exercise metabolism and performance despite enhanced gly cogen availability[J ] . J Physiol, 2021, 599(3): 771-790. DOI: 10.1113/JP280221.

SHI D, HAN T S, CHU X, et al . An isocaloric moderately high-fat diet extends lifespan in male rats and Drosophila [J ] . Cell Metab, 2021, 33(3): 581-597. e9. DOI: 10.1016/j.cmet.2020.12.017..

郑佳伟, 刘武剑, 朱俊东, 等. 紫檀芪和跑台运动诱导大鼠白色脂肪棕色化的作用[J]. 第三军医大学学报, 2020, 42(4): 367-374. DOI: 10.16016/j.1000-5404.201910115.

ZHENG J W, LIU W J, ZHU J D, et al . Pterostilbene and treadmill exercise induce white adipose browning in rats[J ] . J Third Mil Med Univ, 2020, 42(4): 367-374. DOI: 10.16016/j.1000-5404.201910115.

覃飞, 董亚南, 王松涛, 等. 10月龄Wistar大鼠最大摄氧量测试方案的比较研究[J]. 中国体育科技, 2018, 54(5): 65-69. DOI: 10.16470/j.csst.201805010.

QIN F, DONG Y N, WANG S T, et al . The comparative study on the maximum oxygen uptake test of 10-month old wistar rats[J ] . China Sport Sci Technol, 2018, 54(5): 65-69. DOI: 10.16470/j.csst.201805010.

TOURON J, PERRAULT H, JULIAN V, et al . Impact of eccentric or concentric training on body composition and energy expenditure[J ] . Med Sci Sports Exerc, 2019, 51(9): 1944-1953. DOI: 10.1249/MSS.0000000000001992.

WANG L Q, XU F, SONG Z F, et al . A high fat diet with a high C18: 0/C16: 0 ratio induced worse metabolic and transcriptomic profiles in C57BL/6 mice[J ] . Lipids Health Dis, 2020, 19(1): 172. DOI: 10.1186/s12944-020-01346-z.

HARGREAVES M, SPRIET L L. Skeletal muscle energy metabolism during exercise[J]. Nat Metab, 2020, 2(9): 817-828. DOI: 10.1038/s42255-020-0251-4.

FRITZEN A M, LUNDSGAARD A M, KIENS B. Tuning fatty acid oxidation in skeletal muscle with dietary fat and exercise[J]. Nat Rev Endocrinol, 2020, 16(12): 683-696. DOI: 10.1038/s41574-020-0405-1.

MANA M D, HUSSEY A M, TZOUANAS C N, et al . High-fat diet-activated fatty acid oxidation mediates intestinal stemness and tumorigenicity[J ] . Cell Rep, 2021, 35(10): 109212. DOI: 10.1016/j.celrep.2021.109212.

BRUN J F, MYZIA J, VARLET-MARIE E, et al . Beyond the calorie paradigm: taking into account in practice the balance of fat and carbohydrate oxidation during exercise?[J ] . Nutrients, 2022, 14(8): 1605. DOI: 10.3390/nu14081605.

HETLELID K J, PLEWS D J, HEROLD E, et al . Rethinking the role of fat oxidation: substrate utilisation during high-intensity interval training in well-trained and recreationally trained runners[J ] . BMJ Open Sport Exerc Med, 2015, 1(1): e000047. DOI: 10.1136/bmjsem-2015-000047.

PUCHALSKA P, CRAWFORD P A. Multi-dimensional roles of ketone bodies in fuel metabolism, signaling, and therapeutics[J]. Cell Metab, 2017, 25(2): 262-284. DOI: 10.1016/j.cmet.2016.12.022.

EVANS M, COGAN K E, EGAN B. Metabolism of ketone bodies during exercise and training: physiological basis for exogenous supplementation[J]. J Physiol, 2017, 595(9): 2857-2871. DOI: 10.1113/JP273185.

MCSWINEY F T, WARDROP B, HYDE P N, et al . Keto-adaptation enhances exercise performance and body composition responses to training in endurance athletes[J ] . Metabolism, 2018, 83: e1-e2. DOI: 10.1016/j.metabol.2017.11.016.

MURPHY N E, CARRIGAN C T, MARGOLIS L M. High-fat ketogenic diets and physical performance: a systematic review[J]. Adv Nutr, 2021, 12(1): 223-233. DOI: 10.1093/advances/nmaa101.

RYNDERS C A, BLANC S, DEJONG N, et al . Sedentary behaviour is a key determinant of metabolic inflexibility[J ] . J Physiol, 2018, 596(8): 1319-1330. DOI: 10.1113/jp273282.

BAUGH M E, BOWSER S M, MCMILLAN R P, et al . Postprandial skeletal muscle metabolism following a high-fat diet in sedentary and endurance-trained males[J ] . J Appl Physiol, 2020, 128(4): 872-883. DOI: 10.1152/japplphysiol.00576.2019.

MA S H, HUANG Q Y, YADA K, et al . An 8-week ketogenic low carbohydrate, high fat diet enhanced exhaustive exercise capacity in mice[J ] . Nutrients, 2018, 10(6): 673. DOI: 10.3390/nu10060673.

PETRIDOU A, MOUGIOS V. Exercise to lower postprandial lipemia: why, when, what and how[J]. Int J Sports Med, 2022, 43(12): 1013-1022. DOI: 10.1055/a-1810-5118.

RUAN L, LI F H, LI S B, et al . Effect of different exercise intensities on hepatocyte apoptosis in HFD-induced NAFLD in rats: the possible role of endoplasmic reticulum stress through the regulation of the IRE1/JNK and eIF2 α /CHOP signal pathways[J ] . Oxid Med Cell Longev, 2021, 2021: 6378568. DOI: 10.1155/2021/6378568..

MUSCELLA A, STEFÀNO E, MARSIGLIANTE S. The effects of exercise training on lipid metabolism and coronary heart disease[J]. Am J Physiol Heart Circ Physiol, 2020, 319(1): H76-H88. DOI: 10.1152/ajpheart.00708.2019.

BÁRÁNY P. Muscle abnormalities with kidney failure[J]. Clin J Am Soc Nephrol, 2021, 16(11): 1613-1614. DOI: 10.2215/CJN.12550921.

MILLS S, CANDOW D G, FORBES S C, et al . Effects of creatine supplementation during resistance training sessions in physically active young adults[J ] . Nutrients, 2020, 12(6): 1880. DOI: 10.3390/nu12061880.

BOENO F P, PATEL J, MONTALVO R N, et al . Effects of exercise preconditioning on doxorubicin-induced liver and kidney toxicity in male and female rats[J ] . Int J Mol Sci, 2023, 24(12): 10222. DOI: 10.3390/ijms241210222.

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