过度劳累对小鼠脾脏造血功能的影响

邓三春; 陈苏衡; 于恺华; 郭鸿; 廖阅; 鲍振星; 李玉兰

doi:10.16016/j.2097-0927.202308043

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过度劳累对小鼠脾脏造血功能的影响

基础医学 | 更新时间：2026-01-09

- 过度劳累对小鼠脾脏造血功能的影响
- Effect of overwork on hemopoietic function of spleen in mice
- 陆军军医大学学报 2024年46卷第5期页码：427-433
- 作者机构：
  
  1.730000 兰州，兰州大学第一临床医学院
  2.730000 兰州，兰州大学第一医院麻醉科
- 作者简介：
  
  [李玉兰，E-mail: jasm@sina.com LI Yulan, E-mail: jasm@sina.com
- 基金信息：
  
  甘肃省卫生健康行业科研项目(GSWSKY2021-079)
- DOI：10.16016/j.2097-0927.202308043
  中图分类号： R331.21;R363.22;R873
- 收稿：2023-08-11，
  
  修回：2023-09-06，
  
  纸质出版：2024-03-15
- 稿件说明：
移动端阅览
邓三春, 陈苏衡, 于恺华, 等. 过度劳累对小鼠脾脏造血功能的影响[J]. 陆军军医大学学报, 2024,46(5):427-433.

Sanchun DENG, Suheng CHEN, Kaihua YU, et al. Effect of overwork on hemopoietic function of spleen in mice[J]. Journal of Army Medical University, 2024, 46(5): 427-433.
邓三春, 陈苏衡, 于恺华, 等. 过度劳累对小鼠脾脏造血功能的影响[J]. 陆军军医大学学报, 2024,46(5):427-433. DOI： 10.16016/j.2097-0927.202308043.

Sanchun DENG, Suheng CHEN, Kaihua YU, et al. Effect of overwork on hemopoietic function of spleen in mice[J]. Journal of Army Medical University, 2024, 46(5): 427-433. DOI： 10.16016/j.2097-0927.202308043.

摘要

目的

探讨过度劳累(过劳)对小鼠脾脏造血功能的影响。

方法

C57BL/6J小鼠18只，按随机数字表法分为3组(

)：对照(C)组、过劳15 d (W1) 组、过劳30 d(W2)组。W1、W2组每日强迫水中站立8 h+束缚3 h，分别持续15、30 d，C组无特殊处理。观察测量小鼠一般情况、进食量和体质量，造模结束后取血、处死小鼠，分离脾脏和心脏，测量脾脏指数；检测血常规；脾脏行HE染色；采用RT-qPCR法检测小鼠脾脏趋化因子CXC配体12(C-X-C motif chemokin ligand 12

CXCL12)、干细胞因子(stem cell factor

SCF)和心脏间隙连接蛋白45(connexin 45，Cx45) mRNA表达水平；采用ELISA检测脾脏CXCL12、SCF蛋白表达水平；免疫组化法行心脏Cx45蛋白定量。

结果

① 一般情况：与C组相比，W1组、W2组小鼠毛发粗糙，活跃程度先增加后下降，扎堆、精神状态差；小鼠进食量：W1、W2组显著高于同时间C组；C组小鼠体质量随喂养时间增加而增加，W1、W2组体质量无明显增长；W2组小鼠脾脏指数显著下降(

＜0.05)；②血常规：与C组相比，W2组WBC、RBC数目、HGB浓度显著升高(

＜0.05)；W1组、W2组PLT数目显著升高(

＜0.05)；③脾脏HE染色：与C组相比，W2组小鼠脾脏红髓与白髓分界欠清，红髓区域增大；④RT-qPCR检测结果显示：W2组心脏组织Cx45 mRNA表达显著高于C组(

＜0.05)；脾脏组织SCF mRNA表达显著高于C组和W1组(

＜0.05)；⑤免疫组化检测结果显示：W1组和W2组小鼠心肌Cx45蛋白含量显著高于C组(

＜0.05)；⑥ELISA检测结果显示：W2组脾脏组织SCF蛋白表达显著高于C组和W1组(

＜0.05)，W1组较C组差异无统计学意义。

结论

过劳可破坏心肌细胞间的紧密连接，增强脾脏造血功能，使全血细胞数目过度增加，形成血液高凝状态，这可能是过劳死相关心血管疾病的诱发因素之一。

Abstract

Objective

To investigate the effect of overwork on the hemopoietic function of spleen in mice.

Methods

A total of 18 C57BL/6J mice were randomly divided into 3 groups

i.e.

control group (C)

15-day-overworked group (W1) and 30-day-overworked group (W2). The mice from Groups W1 and W2 were forced to keep standing in water for 8 h and restrained for 3 h for consecutive 15 and 30 d

respectively

while those of Group C had no special treatment. The general condition

food intake and body weight of the mice were observed and measured. After modelling

the mice were euthanatized

and the spleen and heart were isolated to calculate spleen index and observe spleen pathological changes after HE staining

and then blood routine test was performed. The mRNA expression levels of CXCL12 and SCF in the spleen and that of connexin 45 (Cx45) in the heart were detected by RT-qPCR

and the protein levels of CXCL12 and SCF in spleen were detected by ELISA. The cardiac protein level of Cx45 was quantified with immunohistochemical assay.

Results

① General condition showed that Groups W1 and W2 showed coarse hairs

increased at first and then decreased in activities

poor mental state

and larger food intake when compared with Group C. The body weight of Group C was increased with the feeding time

but that of Groups W1 and W2 had no such change. The spleen index was decreased significantly in Group W2 (

0.05). ② The results of blood routine test showed that WBC and RBC counts and HGB level were significantly increased in Group W2(

0.05)

and PLT count in Groups W1 and W2 was significantly increased compared with Group C (

0.05). ③ Spleen HE staining displayed that the boundary between red pulp and white pulp was not clear

and the region of red pulp was enlarged in Group W2 when compared with Group C. ④ RT-qPCR showed that the mRNA level of Cx45 was significantly higher in heart tissue of Group W2 than that of Group C (

0.05)

and that of SCF in spleen tissue of Group W2 was also higher than that in Groups C and W1 (

0.05). ⑤ Immunohistochemical assay indicated that the protein expression of Cx45 in myocardium of Groups W1 and W2 was significantly higher than that in Group C (

0.05). ⑥ ELISA revealed that the content of SCF in the spleen of Group W2 was notably higher than that of Groups C and W1 (

0.05)

but there was no statistical difference between Groups W1 and C.

Conclusion

Overwork destroys the tight connection between cardiac myocytes

enhances the hemopoietic function of spleen

increases WBC count

and then forms a hypercoagulable state

which may be one of the precipitating factors for cardiovascular diseases related to overwork.

关键词

Keywords

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