模拟直升机低频振动致大鼠腰痛模型的建立与鉴定

刘红珍; 徐洋杰; 赵秦; 齐光辉; 王海燕; 李森; 刘媛; 谭波涛; 殷樱; 杨策

doi:10.16016/j.2097-0927.202307074

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模拟直升机低频振动致大鼠腰痛模型的建立与鉴定

专题报道: 陆军航空医学 | 更新时间：2026-01-09

- 模拟直升机低频振动致大鼠腰痛模型的建立与鉴定
- Establishment and identification of a rat model of low back pain induced by simulated helicopter low-frequency vibration
- 陆军军医大学学报 2024年46卷第1期页码：58-65
- 作者机构：
  
  1.400010 重庆，重庆医科大学附属第二医院康复医学科
  2.362100 福建泉州，73159部队91分队
  3.400042 重庆，陆军军医大学(第三军医大学)大坪医院特殊环境战伤防治研究室，创伤与化学中毒全国重点实验室
- 作者简介：
  
  [殷樱，E-mail: 300735@cqmu.edu.cn YIN Ying, E-mail: 300735@cqmu.edu.cn
- 基金信息：
  
  院士牵头科技创新引导专项(2022YSZX-JCX0015CSTB);陆军特色医学中心人才创新能力培养计划(ZXZYTSYS11)
- DOI：10.16016/j.2097-0927.202307074
  中图分类号： R-332;R277.7;R852.25
- 收稿：2023-07-14，
  
  修回：2023-10-30，
  
  纸质出版：2024-01-15
- 稿件说明：
移动端阅览
刘红珍, 徐洋杰, 赵秦, 等. 模拟直升机低频振动致大鼠腰痛模型的建立与鉴定[J]. 陆军军医大学学报, 2024,46(1):58-65.

Hongzhen LIU, Yangjie XU, Qin ZHAO, et al. Establishment and identification of a rat model of low back pain induced by simulated helicopter low-frequency vibration[J]. Journal of Army Medical University, 2024, 46(1): 58-65.
刘红珍, 徐洋杰, 赵秦, 等. 模拟直升机低频振动致大鼠腰痛模型的建立与鉴定[J]. 陆军军医大学学报, 2024,46(1):58-65. DOI： 10.16016/j.2097-0927.202307074.

Hongzhen LIU, Yangjie XU, Qin ZHAO, et al. Establishment and identification of a rat model of low back pain induced by simulated helicopter low-frequency vibration[J]. Journal of Army Medical University, 2024, 46(1): 58-65. DOI： 10.16016/j.2097-0927.202307074.

摘要

目的

通过模拟直升机低频振动建立急性腰痛(acute low back pain

ALBP)模型，探讨陆军飞行人员急性腰痛的成因，为其发生机制和防护提供可靠的动物模型与评估手段。

方法

选取48只8周龄雄性SD大鼠[体质量(200±20) g]，采用随机数字表法分为造模A(振动1 h)、B(振动3 h)、C(振动6 h)组和D(空白对照)组，每组12只。连续5 d将大鼠分别暴露于10 Hz的六自由度振动台1、3和6 h。在造模前和造模后第1、4、7和14天行von Frey测试、旷场实验、转棒疲劳实验、步态分析、24 h摄食量等行为学检测；光学显微镜观察多裂肌组织学变化。

结果

行为学结果显示，持续性低频振动暴露大鼠缩足反射阈值降低(

0.01)、24 h摄食量减少(

0.01)、直立次数减少(

0.05)、转棒跌落速度降低(

0.05)、支撑时比较缩短(

0.05)、足印面积减小(

0.05)、步行速度降低(

0.05)。多裂肌组织学显示细胞水肿，肌细胞形态紊乱且大小不一，排列不均，肌间隙细胞核增多，伴有大量炎性细胞浸润、聚集。

结论

持续低频振动环境暴露会导致大鼠腰痛相关行为学和腰部多裂肌组织学病变。

Abstract

Objective

To establish an acute low back pain (ALBP) model by simulating low-frequency vibration of helicopters and explore the causes of ALBP in army aviation pilots in order to provide a reliable animal model and evaluation method for its pathogenesis and protection.

Methods

Forty-eight male SD rats (8 weeks old

weighing 200±20 g) were selected and randomly divided into groups A

B and C

with vibration for 1

3 and 6 h

respectively

and group D as blank control

with 12 rats in each group. The rats were fixed in sitting posture on a vibrating table (10 Hz

with 6 degree vibration) for corresponding durations during 5 consecutive days. Animal behavioral tests were performed before and on days 1

7 and 14 after modelling

including paw withdrawal threshold

open field test

rotarod fatigue test

gait analysis

and 24-hour food intake assessment. Finally

light microscopy was used to observe the morphological structure of the multifidus muscle.

Results

Behavioral examinations revealed that persistent low-frequency vibration resulted in decreased foot-contraction reflex thresholds (

0.01)

amount of 24-hour food consumption (

0.01)

count of upright standing (

0.05)

rotarod velocity at rat falling off (

0.05)

duty cycle (

0.05)

footprint surface area (

0.05)

and walking speed (

0.05). Histological observation for the multifidus muscle demonstrated cellular edema and myocyte disorganization accompanied by inflammatory cell infiltration and aggregation.

Conclusion

Continuous exposure to a low-frequency vibration leads to significant low back pain-related behaviors and histological changes in the lumbar multifidus muscle of rats.

关键词

Keywords

references

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