维生素A缺乏加重胎粪吸入综合征大鼠肺损伤

李静; 唐佳; 李宛卫; 杨信; 付宏; 胡章雪

doi:10.16016/j.2097-0927.202306071

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维生素A缺乏加重胎粪吸入综合征大鼠肺损伤

基础医学 | 更新时间：2026-01-09

- 维生素A缺乏加重胎粪吸入综合征大鼠肺损伤
- Vitamin A deficiency aggravates lung injury in rats with meconium aspiration syndrome
- 陆军军医大学学报 2023年45卷第20期页码：2171-2178
- 作者机构：
  
  1.400042 重庆，陆军特色医学中心儿科
  2.402260 重庆江津，重庆大学附属江津医院儿科
- 作者简介：
  
  [胡章雪, E-mail：huzx1@163.com HU Zhangxue, E-mail: huzx1@163.com
- 基金信息：
  
  重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0457)
- DOI：10.16016/j.2097-0927.202306071
  中图分类号： R-332;R591.41;R725.641
- 收稿：2023-06-15，
  
  修回：2023-09-19，
  
  纸质出版：2023-10-30
- 稿件说明：
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李静, 唐佳, 李宛卫, 等. 维生素A缺乏加重胎粪吸入综合征大鼠肺损伤[J]. 陆军军医大学学报, 2023,45(20):2171-2178.

Jing LI, Jia TANG, Wanwei LI, et al. Vitamin A deficiency aggravates lung injury in rats with meconium aspiration syndrome[J]. Journal of Army Medical University, 2023, 45(20): 2171-2178.
李静, 唐佳, 李宛卫, 等. 维生素A缺乏加重胎粪吸入综合征大鼠肺损伤[J]. 陆军军医大学学报, 2023,45(20):2171-2178. DOI： 10.16016/j.2097-0927.202306071.

Jing LI, Jia TANG, Wanwei LI, et al. Vitamin A deficiency aggravates lung injury in rats with meconium aspiration syndrome[J]. Journal of Army Medical University, 2023, 45(20): 2171-2178. DOI： 10.16016/j.2097-0927.202306071.

摘要

目的

探讨维生素A(vitamin A，VA)缺乏在新生大鼠胎粪吸入综合征(meconium aspiration syndrome，MAS)中对肺损伤的影响。

方法

通过饲养VA缺乏及VA正常母鼠获得新生7 d VA缺乏SD大鼠(vitamin A deficiency，VAD)及VA正常新生SD大鼠(vitamin A normal，VAN)各25只，分别建立VA缺乏MAS模型(VAD组)及VA正常MAS模型(VAN组)。记录、对比、分析各组新生SD大鼠血气分析、肺组织湿/干质量比、HE染色评估肺组织损伤；ELISA检测肺组织SP-A蛋白、SP-C蛋白、IL-6、血管内皮生长因子(vascular endothelial growth factor，VEGF)、肿瘤坏死因子-α(tumor necrosis factor-α，TNF-α)含量；高效液相色谱检测肺组织β-丙氨酸及其他代谢组物指标。

结果

与VAN组比较，VAD组新生大鼠血气中pH值(

=0.003)、碱剩余(bas

e excess，BE)(

=0.019)显著降低，差异有统计学意义；VAD组血气中CO

分压(PCO

)(

=0.049)、肺组织的湿/干质量比(

=0.001)、IL-6(

=0.002)、VEGF(

=0.003)、TNF-α(

=0.019)显著增高，提示VAD组肺组织炎症反应更重。代谢组学分析表明：两组之间主要差异信号通路包括β-丙氨酸代谢通路、cGMP-PKG信号通路、嗅觉转导通路、神经活性配体-受体相互作用通路等，进一步通过液相色谱质谱技术检测并验证发现VAD组较VAN组中β-丙氨酸(

=0.000)显著上调。

结论

VA缺乏可通过上调MAS的炎症因子的表达加重肺损伤，这可能与VA缺乏后导致β-丙氨酸代谢通路改变有关。

Abstract

Objective

To investigate the effect of vitamin A (VA) deficiency on lung injury in meconium aspiration syndrome (MAS) in newborn rats.

Methods

After pregnant rats were fed with normal and deficient VA diet

25 VA-deficient (VAD) and 25 VA-normal (VAN) neonatal SD rats (7 days old) were obtained

respectively. These rats were used to establish MAS mode. The results of blood gas analysis and wet/dry mass ratio of lung tissue were recorded

compared and analyzed in each group. HE staining was employed to evaluate lung tissue injury. The contents of SP-A protein

SP-C protein

IL-6

vascular endothelial growth factor (VEGF) and tumor necrosis factor-α (TNF-α) in lung tissue were detected by ELISA. β-alanine and other metabolites in the lung tissue was measured with high performance liquid chromatography (HPLC).

Results

The pH value (

=0.003) and base excess (BE) (

=0.019) were significantly lower in the newborn rats from the VAD group than the VAN group. The VAD group had obviously higher partial pressure of carbon dioxide (PCO

) (

=0.049)

wet/dry mass ratio of lung tissue (

=0.001)

and contents of IL-6 (

=0.002)

VEGF (

=0.003) and TNF-α (

=0.019) than the VAD group

suggesting more serious lung inflammation in the VAD group. Furth

er metabolomic analysis showed that the major signaling pathways between VAD and VAN groups included β-alanine metabolism

cGMP-PKG signaling pathway

olfactory transduction pathway

and neural active ligand-receptor interaction pathway. The result of HPLC indicated that β-alanine was notably up-regulated in rats from the VAD group than the VAN group (

=0.000).

Conclusion

VAD can aggravate lung injury by up-regulating MAS inflammatory factors

which may be related to the changes in β-alanine metabolic pathways caused by VAD.

关键词

Keywords

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