环状RNA mmu_circ_0005019影响小鼠心肌细胞钙激活钾通道电流及动作电位

杨岚清; 邬娜; 陈鹏慧; 袁志权; 李成英; 吴龙; 钟理; 李亚斐

doi:10.16016/j.2097-0927.202306024

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环状RNA mmu_circ_0005019影响小鼠心肌细胞钙激活钾通道电流及动作电位

基础医学 | 更新时间：2026-01-09

- 环状RNA mmu_circ_0005019影响小鼠心肌细胞钙激活钾通道电流及动作电位
- Circular RNA mmu_circ_0005019 regulates small conductance calcium-activated potassium current and action potential duration in mouse cardiomyocytes
- 陆军军医大学学报 2024年46卷第2期页码：100-109
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)：军事预防医学系军队流行病学教研室
  2.400038 重庆，陆军军医大学(第三军医大学)：基础医学院神经生物学教研室
  3.401120 重庆，重庆医科大学附属第三医院心血管疾病中心
- 作者简介：
  
  [李亚斐，E-mail: liyafei2008@hotmail.comLI Yafei, E-mail: liyafei2008@hotmail.com
- 基金信息：
  
  国家自然科学基金面上项目(82073649)
- DOI：10.16016/j.2097-0927.202306024
  中图分类号： R322.11;R329.25;R394.3
- 收稿：2023-06-06，
  
  修回：2023-07-31，
  
  纸质出版：2024-01-30
- 稿件说明：
移动端阅览
杨岚清, 邬娜, 陈鹏慧, 等. 环状RNA mmu_circ_0005019影响小鼠心肌细胞钙激活钾通道电流及动作电位[J]. 陆军军医大学学报, 2024,46(2):100-109.

Lanqing YANG, Na WU, Penghui CHEN, et al. Circular RNA mmu_circ_0005019 regulates small conductance calcium-activated potassium current and action potential duration in mouse cardiomyocytes[J]. Journal of Army Medical University, 2024, 46(2): 100-109.
杨岚清, 邬娜, 陈鹏慧, 等. 环状RNA mmu_circ_0005019影响小鼠心肌细胞钙激活钾通道电流及动作电位[J]. 陆军军医大学学报, 2024,46(2):100-109. DOI： 10.16016/j.2097-0927.202306024.

Lanqing YANG, Na WU, Penghui CHEN, et al. Circular RNA mmu_circ_0005019 regulates small conductance calcium-activated potassium current and action potential duration in mouse cardiomyocytes[J]. Journal of Army Medical University, 2024, 46(2): 100-109. DOI： 10.16016/j.2097-0927.202306024.

摘要

目的

探索环状RNA mmu_circ_0005019调控小电导钙激活钾(small-conductance calcium-activated potassium，SK)通道蛋白亚基SK3编码基因Kcnn3的表达，以及对小电导钙激活钾通道电流(I

)和动作电位时程(action potential duration，APD)的影响。

方法

在小鼠HL-1细胞中分别构建mmu_circ_0005019过表达和干扰模型，分为过表达组(

=3)、空质粒组(

=3)、干扰1组(

=3)、干扰2组(

=3)、对照组(

=3)，通过RT-qPCR、Western blot分析mmu_circ_0005019调节Kcnn3表达的分子机制，应用膜片钳技术电流钳模式记录全细胞的I

，并用电压钳模式记录APD。

结果

成功构建了环状RNA mmu_circ_0005019过表达和干扰的HL-1细胞模型。过表达组的Kcnn3基因表达与空质粒组相比明显上调(

0.05)；干扰1组和干扰2组的Kcnn3基因表达与对照组相比均明显下调(

0.05)。电生理发现过表达mmu_circ_0005019增加了HL-1细胞I

电流密度，APD显著缩短；相反，干扰mmu_circ_0005019减少了I

电流密度，APD显著延长。

结论

mmu_circ_0005019可以上调小鼠HL-1细胞Kcnn3的表达水平，从而改变I

和APD，提示环状RNA mmu_circ_0005019可能在房颤发生中起到促进作用。

Abstract

Objective

To investigate the effect of mmu_circ_0005019 on regulating Kcnn3 expression

small conductance calcium-activated potassium current (I

) which is generated by the small conductance calcium-ac

tivated potassium (SK) channel that is partly coded by Kcnn3

and action potential duration (APD).

Methods

We transfected pcDNA3.1-mmu_circ_0005019 expressing vectors and 2 independent small interfering RNAs into HL-1 cells to establish gain- and loss-of-expression cell models

respectively. The transfected cells were divided into overexpression group(

=3)

empty vector group(

=3)

interference 1 group(

=3)

interference 2 group(

=3)

and blank control group(

=3). RT-qPCR and Western blotting were used to determine the expression of Kcnn3. Whole-cell patch clamp technology was performed to record the I

and APD. The effects of mmu_circ_0005019 on Kcnn3 expression

and APD were observed.

Results

Gain- and loss-of-expression cell models were established successfully. The expression of Kcnn3 and I

current density significantly increased

and the APD was shortened in overexpression group compared with empty vector group(

0.05). The expression of Kcnn3 and I

current density were significantly decreased

and the APD was prolonged in interference groups compared with blank control group(

0.05).

Conclusion

Mmu_circ_0005019 promotes the expression of Kcnn3 and its coding channel protein

which regulates I

and APD in mouse cardiomyocytes

and may play a promoting role in the occurrence of atrial fibrillation.

关键词

Keywords

references

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