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小鼠胚肾发育过程中细胞衰老相关基因差异表达分析
Differential expression of genes related to cellular senescence during embryonic kidney development in mice
- 陆军军医大学学报 2024年46卷第10期 页码：1083-1091
- 作者机构：
400042 重庆，陆军特色医学中心肾内科，创伤与化学中毒国家重点实验室
- 作者简介：
[陈佳，E-mail: chenjiasnk@163.com CHEN Jia, E-mail: chenjiasnk@163.com
- 基金信息：重庆市自然科学基金面上项目(CSTB2022NSCQ-MSX0860);创伤、烧伤与复合伤国家重点实验室开放课题(SKLKF202202)
DOI：10.16016/j.2097-0927.202303097
中图分类号： R321.5;R322.61;R394.1收稿：2023-03-15，
修回：2023-09-05，
纸质出版：2024-05-30
- 稿件说明：
移动端阅览
罗佳, 汪晓月, 喻芳, 等. 小鼠胚肾发育过程中细胞衰老相关基因差异表达分析[J]. 陆军军医大学学报, 2024,46(10):1083-1091.
Jia LUO, Xiaoyue WANG, Fang YU, et al. Differential expression of genes related to cellular senescence during embryonic kidney development in mice[J]. Journal of Army Medical University, 2024, 46(10): 1083-1091.
罗佳, 汪晓月, 喻芳, 等. 小鼠胚肾发育过程中细胞衰老相关基因差异表达分析[J]. 陆军军医大学学报, 2024,46(10):1083-1091. DOI： 10.16016/j.2097-0927.202303097.
Jia LUO, Xiaoyue WANG, Fang YU, et al. Differential expression of genes related to cellular senescence during embryonic kidney development in mice[J]. Journal of Army Medical University, 2024, 46(10): 1083-1091. DOI： 10.16016/j.2097-0927.202303097.
摘要
目的
2
通过转录组学筛选胚肾发育过程中的差异表达基因，采用生物信息学分析细胞衰老途径及其相关基因，进一步探讨细胞衰老与胚肾发育的关系。
方法
2
分别取胚龄为14.5 d(E14.5组)和18.5 d(E18.5组)的小鼠肾脏组织，使用过碘酸雪夫染色观察肾脏组织结构，通过转录组学分析胚肾发育过程中基因谱表达变化，采用生物信息学分析细胞衰老相关基因表达变化，并采用实时定量荧光PCR及Western blot验证靶基因的表达水平。
结果
2
随着胚肾的发育，肾脏结构逐渐成熟，转录组学结果及生物信息学分析发现细胞衰老相关基因在胚肾发育过程中发挥重
要作用，其中E18.5组中细胞衰老相关基因
Cdkn
1
a
(P21)mRNA及蛋白相对表达水平较E14.5组升高(
P
<
0.05)，而
Skp
2、
Ccne
1、
Ccnd
2和
Cdk
4 mRNA表达水平较E14.5组降低(
P
<
0.05)。
结论
2
在胚肾发育过程中细胞衰老相关基因表达水平发生显著变化，表明细胞衰老在胚肾发育过程中发挥重要作用。
Abstract
Objective
2
To analyze the cellular senescence pathways and their associated genes with bioinformatic analysis by screening differentially expressed genes (DEGs) during embryonic kidney development with aid of transcriptomics in order to explore the relationship between cellular senescence and embryonic kidney development.
Methods
2
After the kidney tissues from mice at embryonic age of 14.5 days (E14.5 group) and 18.5 days (E18.5 group) were collected
the kidney tissue structure was observed with periodic acid Schiff (PAS) staining
the expression profiles of the related genes during embryonic kidney development were analyzed by transcriptomics
and the changes in the genes related to cellular senescence were further analyzed with bioinformatics. The expression levels of target genes were verified by real-time quantitative fluorescence PCR and Western blotting.
Results
2
With the development of the embryonic kidney
the kidney structure gradually matured. The results of transcriptomic and bioinformatic analyses revealed that cellular senescence-related genes played an important role in the development of embryonic kidney. The mRNA and protein levels of
Cdkn
1
a
(P21)
which was correlated with cellular senescence
were significantly higher (
P
<
0.05)
while the mRNA levels of
Skp
2
Ccne
1
Ccnd
2
and
Cdk4
were decreased in the E18.5 group than the E14.5 group (
P
<
0.05).
Conclusion
2
Obvious changes in the expression levels of cellular senescence-related genes are observed during embryonic kidney development
suggesting that cellular senescence plays an important role in the development.
关键词
Keywords
references
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