质谱法联合网络药理学分析侗药“马镫艾”的抗炎功效

范超; 张在其; 罗莉; 朱俊宇; 梁华平; 郭元彪

doi:10.16016/j.2097-0927.202303087

您当前的位置：

首页 >

文章列表页 >

质谱法联合网络药理学分析侗药“马镫艾”的抗炎功效

药学 | 更新时间：2026-01-09

- 质谱法联合网络药理学分析侗药“马镫艾”的抗炎功效
- Anti-inflammation of Chinese Dong herb Madeng'ai by UPLC-Q-TOF/MS combined with network pharmacology
- 陆军军医大学学报 2023年45卷第14期页码：1509-1519
- 作者机构：
  
  1.610031 成都，西南交通大学附属医院成都市第三人民医院实验医学部
  2.418000 湖南怀化，湖南医药学院侗医药研究湖南省重点实验室
  3.400042 重庆，陆军特色医学中心战伤感染与特需药品研究室，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
  
  [梁华平, E-mail: 13638356728@163.com LIANG Huaping, E-mail: 13638356728@163.com
  [郭元彪, E-mail: guo.ybiao@yahoo.com GUO Yuanbiao, E-mail: guo.ybiao@yahoo.com
- 基金信息：
  
  重庆市博士“直通车”科研项目(CSTB2022BSXM-JCX0009);重庆市自然科学基金面上项目(cstc2021jcyj-msxmX0234)
- DOI：10.16016/j.2097-0927.202303087
  中图分类号： R284.2;R297.2;R319
- 收稿：2023-03-15，
  
  修回：2023-05-29，
  
  纸质出版：2023-07-30
- 稿件说明：
移动端阅览
范超, 张在其, 罗莉, 等. 质谱法联合网络药理学分析侗药“马镫艾”的抗炎功效[J]. 陆军军医大学学报, 2023,45(14):1509-1519.

Chao FAN, Zaiqi ZHANG, Li LUO, et al. Anti-inflammation of Chinese Dong herb Madeng'ai by UPLC-Q-TOF/MS combined with network pharmacology[J]. Journal of Army Medical University, 2023, 45(14): 1509-1519.
范超, 张在其, 罗莉, 等. 质谱法联合网络药理学分析侗药“马镫艾”的抗炎功效[J]. 陆军军医大学学报, 2023,45(14):1509-1519. DOI： 10.16016/j.2097-0927.202303087.

Chao FAN, Zaiqi ZHANG, Li LUO, et al. Anti-inflammation of Chinese Dong herb Madeng'ai by UPLC-Q-TOF/MS combined with network pharmacology[J]. Journal of Army Medical University, 2023, 45(14): 1509-1519. DOI： 10.16016/j.2097-0927.202303087.

摘要

目的

阐明侗药“马镫艾”(Madeng’ai，MDA)根部水提物抑制炎症的潜在药效物质及机制。

方法

首先采用质谱技术对MDA水提物的化学成分进行鉴定，再利用Swiss Target Predict数据库和Omim、Disgenet及Genecards数据库分别筛选出疾病和药物靶点，取交集后获得药物-疾病共同靶点；通过软件Cytoscape 3.9.1构建“药物-成分-疾病-靶点”网络并筛选出核心组分；通过String数据库构建蛋白质-蛋白质相互作用网络(protein-protein interaction networks，PPI)；使用David数据库进行基因本体论(gene ontology，GO)分析与京都基因与基因组百科全书(Kyoto encyclopedia of genes and genomes，KEGG)通路富集分析；最后，采用RT-qPCR和Western blot的方法在体外研究MDA水提物对几种典型炎症因子mRNA水平的影响及可能的信号通路。

结果

质谱法在MDA水提物中一共鉴定出了29种化合物，排名前五位的分别为：委陵菜酸、鞣花酸、原花青素C1、儿茶素和原花青素B1。通过数据库检索并去重后共获得104个药物-疾病共同靶点，其中TNF-

、IL-6、AKT1及ALB等可能是其核心作用靶点。GO功能富集分析一共获取到761个条目，其中与生物学过程相关的共600条，涉及到细胞组分的共52条，涉及到分子功能的共109条；KEGG通路富集分析共得到160条相关信号通路，其中包含NF-κB、MAPK、PI3K-AKT等信号通路。与正常对照组相比，模型组(LPS组)TNF-

、IL-6、IL-1β及CCL2的含量显著升高(

0.05)，p38、ERK及JNK的磷酸化水平显著上调(

0.05)；与模型(LPS)组相比，MDA处理组TNF-

、IL-6、IL-1β及CCL2的含量显著降低(

0.05)，p38、ERK及JNK的磷酸化水平显著下调(

0.05)。

结论

本研究预测了侗药MDA水提物具备多种抗炎活性成分，并且可通过MAPK等多条信号通路发挥抗炎作用。

Abstract

Objective

To explore the potential active components and anti-inflammation mechanism of a Chinese herb from Dong ethnic minority

Madeng'ai (MDA).

Methods

UPLC-Q-TOF-MS was taken to analyze and identify the major components of MDA. The potential active targets of MDA were retrieved in the Swiss Target Prediction database

and the disease-related targets were searched in the Omim

Disgene and Genecards databases. The common targets of active components and inflammation were obtained for the potential targets of MDA in the treatment against inflammation. The "drug-disease-target" network and protein-protein interaction (PPI) network were separately constructed by Cytoscape 3.9.1 and String database. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses of the overlapping targets were performed through David database. Finally

RT-qPCR and Western blotting were conducted to determine the mRNA levels of several inflammation-related factors and the possible signaling pathways in RAW264.7 cells after LPS treatment.

Results

There were 29 compounds identified by UPLC-Q-TOF/MS

and the top 5 were tormentic acid

ellagic acid

procyanidin C1

epicatechin and procyanidin B1. There were 104 core targets of MDA docking inflammation through database retrieval and after duplicate removal

and TNF-

IL-6

AKT1 and ALB might be the core targets. A total of 761 GO items were enriched

including 600 items concerning biological processes

52 items on cell components

and 109 items involving molecular functions. KEGG analysis screened out 160 signal pathways

mainly focusing on NF-κB

MAPK

PI3K-AKT and so forth signaling pathways. Compared with the normal control group

the mRNA levels of TNF-

IL-6

IL-1β and CCL2 were increased significantly in the model group (LPS treatment group)(

0.05)

and the phosphorylation levels of p38

ERK and JNK were obviously up-regulated (

0.05). While

MDA treatment notably declined the mRNA levels of TNF-

IL-6

IL-1β and CCL2 (

0.05)

and down-regulated the phosphorylation levels of p38

ERK and JNK when compared with the model group (

0.05).

Conclusion

Our study forecasts that the water extract of MDA

a Chinese traditional herb from Dong minority

has the potential active ingredients in treatment of inflammation

and shows anti-inflammatory effects through multiple pathways

such as MAPK pathway.

关键词

Keywords

references

HE F, ANTONUCCI L, KARIN M. NRF 2 as a regulator of cell metabolism and inflammation in cancer[J ] . Carcinogenesis, 2020, 41(4): 405-416. DOI: 10.1093/carcin/bgaa039.

YEUNG Y T, AZIZ F, GUERRERO-CASTILLA A, et al . Signaling pathways in inflammation and anti-inflammatory therapies[J ] . Curr Pharm Des, 2018, 24(14): 1449-1484. DOI: 10.2174/1381612824666180327165604.

SOEHNLEIN O, LIBBY P. Targeting inflammation in atherosclerosis—from experimental insights to theclinic[J]. Nat Rev Drug Discov, 2021, 20(8): 589-610. DOI: 10.1038/s41573-021-00198-1.

WÖLFLE U, HOFFMANN J, HAARHAUS B, et al . Anti-inflammatory and vasoconstrictive properties of Potentilla erecta —A traditional medicinal plant from the Northern hemisphere[J ] . J Ethnopharmacol, 2017, 204: 86-94. DOI: 10.1016/j.jep.2017.03.058..

CAPLAN A, FETT N, ROSENBACH M, et al . Prevention and management of glucocorticoid-induced side effects: a comprehensive review: gastrointestinal and endocrinologic side effects[J ] . J Am Acad Dermatol, 2017, 76(1): 11-16. DOI: 10.1016/j.jaad.2016.02.1239.

ZHANG R R, MENG N N, LIU C, et al . PDB-1 from Potentilla discolor Bunge induces apoptosis and autophagy by downregulating the PI3K/Akt/mTOR signaling pathway in A549 cells[J ] . Biomedecine Pharmacother, 2020, 129: 110378. DOI: 10.1016/j.biopha.2020.110378..

LIU W W, ZHANG Y J, ZHU W N, et al . Sinomenine inhibits the progression of rheumatoid arthritis by regulating the secretion of inflammatory cytokines and monocyte/macrophage subsets[J ] . Front Immunol, 2018, 9: 2228. DOI: 10.3389/fimmu.2018.02228.

DHAIBAN S, AL-ANI M, ELEMAM N M, et al . Targeting chemokines and chemokine receptors in multiple sclerosis and experimental autoimmune encephalomyelitis[J ] . J Inflamm Res, 2020, 13: 619-633. DOI: 10.2147/JIR.S270872.

WU J, ZHANG Z Q, ZHOU X D, et al . New terpenoids from Potentilla freyniana Bornm. and their cytotoxic activities[J ] . Molecules, 2022, 27(12): 3665. DOI: 10.3390/molecules 27123665..

CAI W, LI K L, QIN S H, et al . Rapid identification and systematic mechanism of flavonoids from Potentilla freyniana Bornm. based on UHPLC-Q-exactive orbitrap mass spectrometry and network pharmacology[J ] . Int J Anal Chem, 2021, 2021: 6619959. DOI: 10.1155/2021/6619959..

TANG Z R, ZHAO Y N, ZHANG Z Q, et al . Study of the antimicrobial activity of the Chinese Dong ethnic minority medicine, madeng'ai[J ] . Evid Based Complement Alternat Med, 2022, 2022: 3678240. DOI: 10.1155/2022/3678240.

LI W, LUO L, ZHU J Y, et al . Water extract of Cayratia albifolia C.L. Li root relieves zymosan A-induced inflammation by restraining M1 macrophage polarization[J ] . Phytomedicine, 2022, 96: 153901. DOI: 10.1016/j.phymed.2021.153901..

LUO L, ZHANG W, ZHANG Z Q, et al . The water extract of "Jiao Mei Gu" attenuates the lipopolysaccharide-induced inflammatory response via inhibiting NF-κB activity in mice[J ] . J Ethnopharmacol, 2020, 259: 112882. DOI: 10.1016/j.jep.2020.112882.

DONG Y Z, ZHAO Q L, WANG Y G, et al . Network pharmacology-based investigation of potential targets of astragalus membranaceous-angelica sinensis compound acting on diabetic nephropathy[J ] . Sci Rep, 2021, 11(1): 19496. DOI: 10.1038/s41598-021-98925-6.

LI X, WEI S Z, NIU S Q, et al . Network pharmacology prediction and molecular docking-based strategy to explore the potential mechanism of Huanglian Jiedu Decoction against sepsis[J ] . Comput Biol Med, 2022, 144: 105389. DOI: 10.1016/j.compbiomed.2022.105389.

WANG Z Y, WANG X, ZHANG D Y, et al . Traditional Chinese medicine network pharmacology: development in new era under guidance of network pharmacology evaluation method guidance[J ] . China J Chin Materia Medica, 2022, 47(1): 7-17. DOI: 10.19540/j.cnki.cjcmm.20210914.702.

TAO J Y, YAN S L, ZHOU C Y, et al . Total flavonoidsfrom Potentilla kleiniana Wight et Arn inhibits biofilm formation and virulence factors production in methicillin-resistant Staphylococcus aureus (MRSA)[J ] . J Ethnopharmacol, 2021, 279: 114383. DOI: 10.1016/j.jep.2021.114383..

D'ELIA R V, HARRISON K, OYSTON P C, et al . Targeting the cytokine storm for therapeutic benefit[J ] . Clin Vaccine Immunol, 2013, 20(3): 319-327. DOI: 10.1128/CVI.00636-12.

MUNJAL A, KHANDIA R. Atherosclerosis: orchestrating cells and biomolecules involved in its activation and inhibition[M ] //Advances in protein chemistry and structural biology. Amsterdam: Elsevier, 2020: 85-122. DOI: 10.1016/bs.apcsb.2019.11.002 http://dx.doi.org/10.1016/bs.apcsb.2019.11.002 .

BEIZAVI Z, ZOHOURI M, ASADIPOUR M, et al . IL-27, a pleiotropic cytokine for fine-tuning the immune response in cancer[J ] . Int Rev Immunol, 2021, 40(5): 319-329. DOI: 10.1080/08830185.2020.1840565.

SARAIVA M, VIEIRA P, O'GARRA A. Biology and therapeutic potential of interleukin-10[J]. J Exp Med, 2020, 217(1). DOI: 10.1084/jem.20190418.

LIANG W G, TRIANDAFILLOU C G, HUANG T Y, et al . Structural basis for oligomerization and glycosaminoglycan binding of CCL5 and CCL3[J ] . Proc Natl Acad Sci USA, 2016, 113(18): 5000-5005. DOI: 10.1073/pnas.1523981113.

HUA X L, GE S D, ZHANG M, et al . Pathogenic roles of CXCL10 in experimental autoimmune prostatitis by modulating macrophage chemotaxis and cytokine secretion[J ] . Front Immunol, 2021, 12: 706027. DOI: 10.3389/fimmu.2021.706027.

DENG T Y, SHEN P, LI A M, et al . CCDC65 as a new potential tumor suppressor induced by metformin inhibits activation of AKT1 via ubiquitination of ENO1 in gastric cancer[J ] . Theranostics, 2021, 11(16): 8112-8128. DOI: 10.7150/thno.54961.

IGLESIAS D E, CREMONINI E, FRAGA C G, et al . Ellagic acid protects Caco-2 cell monolayers against inflammation-induced permeabilization[J ] . Free Radic Biol Med, 2020, 152: 776-786. DOI: 10.1016/j. freeradbiomed.2020.01.022.

LIN X, WEI Y Y, LI Y, et al . Tormentic acid ameliorates hepatic fibrosis in vivo by inhibiting glycerophospholipids metabolism and PI3K/Akt/mTOR and NF-κB pathways: based on transcriptomics and metabolomics[J ] . Front. Pharmacol, 2022, 13: 801982. DOI: 10.3389/fphar.2022.801982.

SHEINENZON A, SHEHADEH M, MICHELIS R, et al . Serum albumin levels and inflammation[J ] . Int J Biol Macromol, 2021, 184: 857-862. DOI: 10.1016/j.ijbiomac.2021.06.140.

ZHENG K N, LV B J, WU L L, et al . Protecting effect of emodin in experimental autoimmune encephalomyelitis mice by inhibiting microglia activation and inflammation via Myd88/PI3K/Akt/NF-κB signalling pathway[J ] . Bioengineered, 2022, 13(4): 9322-9344. DOI: 10.1080/21655979.2022.2052671.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

MAVS功能丧失性突变导致抗病毒免疫缺陷：揭示病毒感染触发儿童泛发性脓疱性银屑病的新机制

Z-藁本内酯通过抑制PI3K/AKT信号通路延缓视网膜色素变性：基于网络药理学与分子对接验证

ATF3通过抑制ATG5和ATG16L1表达调控巨噬细胞自噬与炎症反应

新型吩噻嗪衍生物DA414在大鼠脑出血中的作用及机制研究

活性氧清除性Ce6-鲁米诺脂质体的制备及其对小鼠放射性肺炎的保护作用研究