PAX1甲基化与p16/Ki-67联合检测提高宫颈癌非典型鳞状细胞的诊断效能

王元培; 刘雨晨; 温静; 孙怡; 程晓冉; 任芳

doi:10.16016/j.2097-0927.202302133

您当前的位置：

首页 >

文章列表页 >

PAX1甲基化与p16/Ki-67联合检测提高宫颈癌非典型鳞状细胞的诊断效能

临床医学 | 更新时间：2026-01-09

- PAX1甲基化与p16/Ki-67联合检测提高宫颈癌非典型鳞状细胞的诊断效能
- Combined detection of PAX1 methylation and p16/Ki-67 dual staining improves diagnostic performance for atypical squamous cells in cervical cancer
- 陆军军医大学学报 2024年46卷第4期页码：391-395
- 作者机构：
  
  450052 郑州，郑州大学第一附属医院妇科
- 作者简介：
  
  [任芳，E-mail: renfang@foxmail.comREN Fang, E-mail: renfang@foxmail.com
- 基金信息：
  
  河南省科技发展计划优秀青年科学基金(222300420091)
- DOI：10.16016/j.2097-0927.202302133
  中图分类号： R446.8;R730.43;R737.33
- 收稿：2023-02-27，
  
  修回：2023-06-07，
  
  纸质出版：2024-02-28
- 稿件说明：
移动端阅览
王元培, 刘雨晨, 温静, 等. PAX1甲基化与p16/Ki-67联合检测提高宫颈癌非典型鳞状细胞的诊断效能[J]. 陆军军医大学学报, 2024,46(4):391-395.

Yuanpei WANG, Yuchen LIU, Jing WEN, et al. Combined detection of PAX1 methylation and p16/Ki-67 dual staining improves diagnostic performance for atypical squamous cells in cervical cancer[J]. Journal of Army Medical University, 2024, 46(4): 391-395.
王元培, 刘雨晨, 温静, 等. PAX1甲基化与p16/Ki-67联合检测提高宫颈癌非典型鳞状细胞的诊断效能[J]. 陆军军医大学学报, 2024,46(4):391-395. DOI： 10.16016/j.2097-0927.202302133.

Yuanpei WANG, Yuchen LIU, Jing WEN, et al. Combined detection of PAX1 methylation and p16/Ki-67 dual staining improves diagnostic performance for atypical squamous cells in cervical cancer[J]. Journal of Army Medical University, 2024, 46(4): 391-395. DOI： 10.16016/j.2097-0927.202302133.

摘要

目的

比较配对盒家族基因1(paired boxed gene 1

PAXl)甲基化与p16/Ki-67双染单独及联合检测在薄层液基细胞学检查(thinprep cytologic test

TCT)为意义不明的非典型鳞状细胞(atypical squamous cells of undetermined significance

ASC-US)及低级别鳞状上皮内病变(low-grade squamous intraepithelial lesion

LSIL)人群中的诊断效能。

方法

选择郑州大学第一附属医院2021-2022年247例TCT结果为ASC-US和LSIL患者

以阴道镜下病理结果为金标准

敏感度、特异性、准确度与ROC曲线下面积(area under the subject curve

AUC)为评价指标

比较PAX1甲基化和p16/Ki-67双染单独及联合检测的检验效能。

结果

PAX1甲基化与p16/Ki-67双染的阳性率随着阴道镜下病理结果的严重程度而增加

PAX1甲基化和p16/Ki-67联合检测在TCT为ASC-US人群中的敏感度为91.25%、特异度为97.72%、准确度为95.51%

优于甲基化、p16/Ki-67双染单独检测

差异有统计学意义(

0.01)。

结论

PAX1甲基化和p16/Ki-67双染联合检测可以提高TCT结果为ASC-US患者的诊断效能。

Abstract

Objective

To compare paired boxed gene 1(PAX1) methylation and p16/Ki-67 double staining alone and in combination in thinprep cytologic test (TCT) for distinguishing atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL) population.

Methods

A total of 247 patients with TCT results of ASC-US and LSIL admitted in our hospital from January 2021 to December 2022 were enrolled in this study.Detection efficacy of PAX1 methylation and p16/Ki-67 double staining alone and in combination was evaluated with colposcopic pathologic results as the gold standard and the sensitivity

specificity

accuracy and area under the curve (AUC) as evaluation indexes.

Results

The positive rates of PAX1 methylation and p16/Ki-67 double staining were increased with the severity of pathological findings.Combined detection of PAX1 methylation and p16/Ki-67 assay had a sensitivity of 91.25%

specificity of 97.72% and accuracy of 95.51% in the women with TCT of ASC-US

and these values were statistically better than those of PAX1 methylation and p16/Ki-67 double staining alone (

0.01).

Conclusion

The combination of PAX1 methylation and p16/Ki-67 double-staining assay can further improve the diagnostic efficacy in patients with ASC-US on TCT results.

关键词

Keywords

references

SUNGH, FERLAY J, SIEGEL R L, et al . Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries[J ] . CA Cancer J Clin, 2021, 71(3): 209-249. DOI: 10.3322/caac.21660.

SIEBERS A G, KLINKHAMER P J, VEDDER J E, et al . Causes and relevance of unsatisfactory and satisfactory but limited smears of liquid-based compared with conventional cervical cytology[J ] . Arch Pathol Lab Med, 2012, 136(1): 76-83. DOI: 10.5858/arpa.2011-0113-OA.

LORINCZ A T. Virtues and weaknesses of DNA methylation as a test for cervical cancer prevention[J]. Acta Cytol, 2016, 60(6): 501-512. DOI: 10.1159/000450595.

BIGRAS G, WILSON J, RUSSELL L, et al . Interobserver concordance in the assessment of features used for the diagnosis of cervical atypical squamous cells and squamous intraepithelial lesions (ASC-US, ASC-H, LSIL and HSIL)[J ] . Cytopathology, 2013, 24(1): 44-51. DOI: 10.1111/j.1365-2303.2011.00930.x.

REN C C, ZHU Y H, YANG L, et al . Prognostic and diagnostic validity of p16/Ki-67, HPV E6/E7 mRNA, and HPV DNA in women with ASCUS: a follow-up study[J ] . Virol J, 2019, 16(1): 143. DOI: 10.1186/s12985-019-1251-4.

张波, 杨志敏, 康子婧, 等. 子宫颈脱落细胞块及p16/Ki-67双染在ASC-US和LSIL分流中的应用进展[J]. 诊断病理学杂志, 2023, 30(2): 194-197. DOI: 10.3969/j.issn.1007-8096.2023.02.024

ZHANG B, YANG Z M, KANG Z J, et al . Application progress of cervical exfoliated cells and p16/Ki-67 double staining in ASC-US and LSIL shunt[J ] . Chin J Diagn Pathol, 2023, 30(2): 194-197. DOI: 10.3969/j.issn.1007-8096.2023.02.024

SAWAYA G F, GRIMES D A. New technologies in cervical cytology screening: a word of caution[J]. Obstet Gynecol, 1999, 94(2): 307-310. DOI: 10.1016/S0029-7844(99)00289-6.

VRDOLJAK-MOZETIČD, KRAŠEVIĆM, VERŠA OSTOJIĆD, et al . HPV16 genotype, p16/Ki-67 dual staining and koilocytic morphology as potential predictors of the clinical outcome for cervical low-grade squamous intraepithelial lesions[J ] . Cytopathology, 2015, 26(1): 10-18. DOI: 10.1111/cyt.12121.

SUN H Z, SHEN K, CAO D Y. Progress in immunocytochemical staining for cervical cancer screening[J]. Cancer Manag Res, 2019, 11: 1817-1827. DOI: 10.2147/cmar.s195349.

LI M Z, YANG J, LIU K Y, et al . p16 promotes proliferation in cervical carcinoma cells through CDK6-HuR-IL1A axis[J ] . J Cancer, 2020, 11(6): 1457-1467. DOI: 10.7150/jca.35479.

PAIXÃO-CÔRTES V R, SALZANO F M, BORTOLINI M C. Origins and evolvability of the PAX family[J]. Semin Cell Dev Biol, 2015, 44: 64-74. DOI: 10.1016/j.semcdb.2015.08.014.

LAI H C, LIN Y W, HUANG T H, et al . Identification of novel DNA methylation markers in cervical cancer[J ] . Int J Cancer, 2008, 123(1): 161-167. DOI: 10.1002/ijc.23519.

LUAN T, HUA Q, LIU X, et al . PAX1 methylation as a potential biomarker to predict the progression of cervical intraepithelial neoplasia: a meta-analysis of related studies[J ] . Int J Gynec Cancer, 2017, 27(7): 1480-1488. DOI: 10.1097/IGC.0000000000001011.

GRENKO R T, ABENDROTH C S, FRAUENHOFFER E E, et al . Variance in the interpretation of cervical biopsy specimens obtained for atypical squamous cells of undetermined significance[J ] . Am J Clin Pathol, 2000, 114(5): 735-740. DOI: 10.1309/K7C9-X5P0-001B-2HK5.

李碧军. P16/Ki-67双染和高危HPV E6/E7mRNA检测在宫颈癌筛查中的应用[D]. 郑州: 郑州大学, 2021.

LI B J. Application of P16/Ki-67 double staining and high-risk HPV E6/E7mRNA detection in cervical cancer screening[D]. Zhengzhou: Zhengzhou University, 2021.

KISSER A, ZECHMEISTER-KOSS I. A systematic review of p16/Ki-67 immuno-testing for triage of low grade cervical cytology[J]. Int J Obstet Gynaecol, 2015, 122(1): 64-70. DOI: 10.1111/1471-0528.13076.

张效东, 苏玥辉, 李盼盼, 等. 配对盒家族基因1甲基化与人乳头瘤病毒E6/E7mRNA检测在子宫颈病变患者诊断中的应用[J]. 实用妇产科杂志, 2021, 37(4): 315-318. DOI: 10.3969/j.issn.1671-878X.2018.01.027.

ZHANG X D, SU Y H, LI P P, et al . Application of methylation of matchbox family gene 1 and detection of human papillomavirus E6/E7mRNA in diagnosis of cervical lesions[J ] . J Pract Obstet Gynecol, 2021, 37(4): 315-318. DOI: 10.3969/j.issn.1671-878X.2018.01.027.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

全身炎症反应指数联合临床病理参数预测宫颈癌复发的模型建立与验证

3D打印模具在宫颈癌近距离治疗中重复使用可行性研究

RNA传感器IFIH1对HPV阳性的宫颈癌细胞增殖、迁移和侵袭的影响

宫颈癌微创根治术患者术后超早期活动的安全性研究

DNA修复基因APE1 Asp148Glu多态性与中国西南地区女性宫颈癌风险及预后相关