三七三醇皂苷主要成分抑制剪切力诱导的血小板活化和聚集

黄小静; 张天聪; 高雪梅; 宦宣容; 李远

doi:10.16016/j.2097-0927.202212175

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三七三醇皂苷主要成分抑制剪切力诱导的血小板活化和聚集

基础医学 | 更新时间：2026-01-09

- 三七三醇皂苷主要成分抑制剪切力诱导的血小板活化和聚集
- Main components of Panax notoginseng saponins inhibit platelet activation and aggregation induced by shear stress
- 陆军军医大学学报 2023年45卷第19期页码：2037-2044
- 作者机构：
  
  402160 重庆，重庆医科大学附属永川医院中心实验室
- 作者简介：
  
  [李远，E-mail: liyuan_1985999@163.comLI Yuan, E-mail: liyuan_1985999@163.com
- 基金信息：
  
  国家自然科学基金青年科学基金(11702047);重庆市科卫联合医学科研项目中青年高端人才项目(2023GDRC008);重庆医科大学附属永川医院研究生创新基金项目(YJSCX202202)
- DOI：10.16016/j.2097-0927.202212175
  中图分类号： R285.5;R331.143;R364.15
- 收稿：2022-12-29，
  
  修回：2023-07-06，
  
  纸质出版：2023-10-15
- 稿件说明：
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黄小静, 张天聪, 高雪梅, 等. 三七三醇皂苷主要成分抑制剪切力诱导的血小板活化和聚集[J]. 陆军军医大学学报, 2023,45(19):2037-2044.

Xiaojing HUANG, Tiancong ZHANG, Xuemei GAO, et al. Main components of Panax notoginseng saponins inhibit platelet activation and aggregation induced by shear stress[J]. Journal of Army Medical University, 2023, 45(19): 2037-2044.
黄小静, 张天聪, 高雪梅, 等. 三七三醇皂苷主要成分抑制剪切力诱导的血小板活化和聚集[J]. 陆军军医大学学报, 2023,45(19):2037-2044. DOI： 10.16016/j.2097-0927.202212175.

Xiaojing HUANG, Tiancong ZHANG, Xuemei GAO, et al. Main components of Panax notoginseng saponins inhibit platelet activation and aggregation induced by shear stress[J]. Journal of Army Medical University, 2023, 45(19): 2037-2044. DOI： 10.16016/j.2097-0927.202212175.

摘要

目的

研究三七三醇皂苷主要成分对剪切力诱导的血小板活化和聚集的影响。

方法

建立狭窄微流控芯片模型，采用微流控芯片技术评价在不同浓度(0.05、0.1、0.25、0.5 mg/mL)及不同剪切率(1 500、4 500、9 000 s

-1

)条件下Rg1、Re和R1对剪切力诱导的血小板聚集影响；采用流式细胞术定量分析Rg1、Re和R1对高剪切力诱导的血小板活化(P-选择素和PAC-1)的影响；采用PL-12分析仪检测Rg1、Re和R1对AA和ADP诱导的血小板聚集的影响。

结果

Rg1、Re和R1呈浓度依赖性抑制高剪切力诱导的血小板聚集(

0.01)，而对较低剪切力无抑制作用，其中Re抑制作用最强，三者的IC

分别为0.1、0.07、0.2 mg/mL，当3种化合物在相同浓度下联合使用时没有观察到协同作用。Rg1、Re和R1抑制P-选择素和PAC-1的表达(

0.01)，抑制效果与聚集实验结果一致。Rg1、Re和R1能够抑制AA和ADP诱导的血小板聚集(

0.05)。

结论

三七三醇皂苷主要成分通过抑制剪切力诱导的血小板聚集发挥新的抗血小板活性。

Abstract

Objective

To determine the effects of main components (Rg1

Re and R1) of

Panax notoginseng

saponins on platelet activation and aggregation induced by shear stress.

Methods

A narrow microfluidic chip model was established

and used to evaluate the effects of

Rg1

Re and R1 at different concentrations (0.05

0.1

0.25 and 0.5 mg/mL) on shear-induced platelet aggregation under different shear rates (1 500

4 500 and 9 000 s

-1

). Flow cytometry was used to quantitatively detect the shear-induced platelet activation (P-selectin and PAC-1) after the treatment of Rg1

Re and R1. PL-12 analyzer was performed to measure the effects of Rg1

Re and R1 on platelet aggregation induced by arachidonic acid (AA) and adenosine diphosphate (ADP).

Results

Rg1

Re and R1 inhibited platelet aggregation induced by high shear stress in a concentration-dependent manner (

0.01)

but had no such inhibitory effect under lower shear stress. Among the 3 components

Re had the strongest inhibitory effect

and their IC

value was 0.1

0.07

and 0.2 mg/mL

respectively. No synergistic effect was observed when the 3 components were used together at the same dose. Rg1

Re and R1 inhibited the expression of P-selectin and PAC-1 (

0.01)

which was consistent with the results of the aggregation experiment. In addition

the 3 components also could inhibit platelet aggregation induced by AA and ADP.

Conclusion

The main components of

Panax notoginseng

saponins exert their antiplatelet activity by inhibiting shear-induced platelet aggregation.

关键词

Keywords

references

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