精索静脉曲张患者肠道菌群的特征组成及功能分析

彭屹松; 刘爽; 唐黎; 肖续; 唐波; 杨仕明

doi:10.16016/j.2097-0927.202212019

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精索静脉曲张患者肠道菌群的特征组成及功能分析

基础医学 | 更新时间：2026-01-09

- 精索静脉曲张患者肠道菌群的特征组成及功能分析
- Diversity and functional characteristics of intestinal microbiome in patients with varicocele
- 陆军军医大学学报 2023年45卷第6期页码：493-499
- 作者机构：
  
  400037 重庆，陆军军医大学(第三军医大学)第二附属医院消化内科
- 作者简介：
  
  [杨仕明，E-mail: shimingyang@yahoo.com YANG Shiming, E-mail: shimingyang@yahoo.com
- 基金信息：
  
  陆军军医大学科技创新能力提升专项军事医学预研项目(2019XYY17);军队医学科技青年培育计划孵化项目(20QNPY013)
- DOI：10.16016/j.2097-0927.202212019
  中图分类号： Q939.121;R394.5;R697.24
- 收稿：2022-12-02，
  
  修回：2023-01-05，
  
  纸质出版：2023-03-30
- 稿件说明：
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彭屹松, 刘爽, 唐黎, 等. 精索静脉曲张患者肠道菌群的特征组成及功能分析[J]. 陆军军医大学学报, 2023,45(6):493-499.

Yisong PENG, Shuang LIU, Li TANG, et al. Diversity and functional characteristics of intestinal microbiome in patients with varicocele[J]. Journal of Army Medical University, 2023, 45(6): 493-499.
彭屹松, 刘爽, 唐黎, 等. 精索静脉曲张患者肠道菌群的特征组成及功能分析[J]. 陆军军医大学学报, 2023,45(6):493-499. DOI： 10.16016/j.2097-0927.202212019.

Yisong PENG, Shuang LIU, Li TANG, et al. Diversity and functional characteristics of intestinal microbiome in patients with varicocele[J]. Journal of Army Medical University, 2023, 45(6): 493-499. DOI： 10.16016/j.2097-0927.202212019.

摘要

目的

利用宏基因组学分析精索静脉曲张(varicocele

VC)患者和健康对照人群的肠道菌群多样性和菌群组成变化。

方法

选取2020年1~12月就诊于陆军军医大学第二附属医院的精索静脉曲张患者(

=15)和健康对照人群(

=15)的新鲜粪便并提取DNA，采用宏基因组学测序分析受试者的肠道菌群多样性、结构特征和功能差异。

结果

两组年龄、BMI差异均无统计学意义(

0.05)。患者组的肠道菌群与对照组的肠道菌群相比，Alpha多样性无显著差异(

0.05)；Beta多样性差异有统计学意义(

＜0.01)。两组优势菌群均为厚壁菌门(

firmicutes

)、拟杆菌门(

bacteroidetes

)、变形菌门(

proteobacteria

)、放线菌门(

actinobacteria

)、梭杆菌门(

fusobacteria

)；在种水平上，患者组的优势菌包括黄杆菌属(

flavonifractor plautii

)、甲烷短

杆菌属(

methanobrevibacter smithii

)、肠杆菌属(

enterobacter roggenkampii

)等，而对照组包括考拉杆菌属(

phascolarctobacterium faecium

)、Romboutsia属(

romboutsia ilealis

)、双歧杆菌属(

bifidobacterium bifidum

)、普雷沃氏菌属(

prevotella jejuni

)、梭菌属(

clostridium perfringens

)等。功能分析发现D-galactarate degradation I、Superpathway of D-glucarate and D-galactarate degradation、D-glucarate degradation I等多条促进D-半乳糖降解的信号通路较对照组显著上调，肠球菌属(

enterococcus faecalis

)、黄杆菌属(

flavonifractor plautii

)、拉乌尔菌属(

raoultella terrigena

)等肠道菌群与上调的信号通路相关联。

结论

精索静脉曲张患者与健康对照人群相比肠道菌群构成有特征性改变，数条促进D-半乳糖降解的信号通路有显著上调，肠道菌群改变可能为精索静脉曲张患者机体功能发生病理改变的机制之一。

Abstract

Objective

To investigate the changes of intestinal microbiome in patients with varicocele (VC) with macrogenomics.

Methods

Fresh feces from 15 VC patients and 15 healthy individuals were collected

and DNA sequences were extracted from the feces. The diversity and functional characteristics were analyzed by metagenome sequencing methods.

Results

There were no statistical differences in age and body mass index (BMI) between the 2 groups (

0.05). The Alpha diversity results showed no significant difference was observed between them (

0.05). The Beta diversity results indicated that the species diversity had significant differences in the feces between the VC patients and healthy individuals (

0.01). The dominant floras in both the VC patients and healthy individuals were mainly

Firmicutes

Bacteroidetes

Proteobacteria

Actinobacteria

and

Fusobacteria

. At species level

the dominant floras in VC patients included

Flavonifractor plautii

Methanobrevibacter smithii

Enterobacter roggenkampii

etc

and those in the healthy individuals were

Phascolarctobacterium faecium

Romboutsia ilealis

Bifidobacterium bifidum

Prevotella jejuni

Clostridium perfringens

etc

. Metagenomics data identified significant up-regulation of D-glucarate degradation pathways

including D-galactarate degradation I

superpathway of D-glucarate and D-galactarate degradation

D-glucarate degradation I

etc

. The alterations were associated with intestinal microbiomes

Enterococcus faecalis

Flavonifractor plautii

Raoultella terrigena

etc

Conclusion

There are obvious characteristics changes in microflora structure between VC patients and healthy controls. Up-regulation of D-glucarate degradation pathways are found in VC patients. These changes of intestinal microbiome might be one of the underlying pathophysiological mechanism of VC.

关键词

Keywords

references

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