雌马酚对绝经后非酒精性脂肪肝病大鼠的骨保护作用

崔涵强; 倪向敏; 张贵明; 徐喆; 李硕; 王建

doi:10.16016/j.2097-0927.202210126

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雌马酚对绝经后非酒精性脂肪肝病大鼠的骨保护作用

基础医学 | 更新时间：2026-01-09

- 雌马酚对绝经后非酒精性脂肪肝病大鼠的骨保护作用
- Protective effect of equol on bone in postmenopausal rats with nonalcoholic fatty liver disease
- 陆军军医大学学报 2023年45卷第5期页码：407-416
- 作者机构：
  
  400037 重庆, 陆军军医大学(第三军医大学)第二附属医院营养科
- 作者简介：
  
  [王建，E-mail: wangjian_1996@aliyun.com WANG Jian, E-mail: wangjian_1996@aliyun.com
- 基金信息：
  
  国家自然基金面上项目(81973040);国家自然基金青年科学基金项目(81602855)
- DOI：10.16016/j.2097-0927.202210126
  中图分类号： R322.71;R575.5;R977.12
- 收稿：2022-10-26，
  
  修回：2023-01-06，
  
  纸质出版：2023-03-15
- 稿件说明：
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崔涵强, 倪向敏, 张贵明, 等. 雌马酚对绝经后非酒精性脂肪肝病大鼠的骨保护作用[J]. 陆军军医大学学报, 2023,45(5):407-416.

Hanqiang CUI, Xiangmin NI, Guiming ZHANG, et al. Protective effect of equol on bone in postmenopausal rats with nonalcoholic fatty liver disease[J]. Journal of Army Medical University, 2023, 45(5): 407-416.
崔涵强, 倪向敏, 张贵明, 等. 雌马酚对绝经后非酒精性脂肪肝病大鼠的骨保护作用[J]. 陆军军医大学学报, 2023,45(5):407-416. DOI： 10.16016/j.2097-0927.202210126.

Hanqiang CUI, Xiangmin NI, Guiming ZHANG, et al. Protective effect of equol on bone in postmenopausal rats with nonalcoholic fatty liver disease[J]. Journal of Army Medical University, 2023, 45(5): 407-416. DOI： 10.16016/j.2097-0927.202210126.

摘要

目的

观察雌马酚(equol，Eq)对绝经后非酒精性脂肪肝病(nonalcoholic fatty liver disease，NAFLD)大鼠的骨保护效应，并探讨其作用机制。

方法

选取6周龄雌性Sprague Dawley(SD)大鼠48只，随机分为对照组、模型组、低剂量Eq组[20 mg/(kg·d)]、中剂量Eq组[40 mg/(kg·d)]、高剂量Eq组[80 mg/(kg·d)]和雌二醇干预组[estradiol，E2，0.25 mg/(kg·d)]，每组8只，对照组给予假手术及正常饮食，其余各组给予卵巢切除术(OVX)合并高脂饮食(HFD)。16周后测量大鼠体质量、体长，计算BMI和Lee’s指数，检测各组大鼠的骨密度和骨微结构变化；麻醉后处死大鼠并取材，检测大鼠血清雌激素、TNF-α和IL-6水平，肝脏和股骨组织学检查观察形态学变化；采用Western blot和qRT-PCR检测肝和骨组织中胰岛素样生长因子-1 (insulin-like growth factor-1

IGF-1)表达水平。

结果

模型组大鼠BMI和Lee’s指数显著升高(

0.05)，肝组织损伤明显，雌激素和骨密度水平较对照组显著下降(

0.05)，骨显微结构破坏严重，提示绝经后NAFLD合并明显骨损伤；Eq和E2干预能显著升高大鼠血清雌激素和骨密度水平(

0.0

5)，改善骨显微结构，降低BMI、Lee’s指数和血清TNF-α、IL-6水平(

0.05)，减轻肝和骨组织脂质累积，升高肝和骨组织IGF-1蛋白和mRNA表达水平(

0.05)。

结论

Eq能改善绝经后NAFLD大鼠的骨损伤，具有骨保护作用，其部分机制可能与Eq促进IGF-1表达和减轻炎症反应有关。

Abstract

Objective

To observe the osteoprotective effect of equol (Eq) on postmenopausal rats with nonalcoholic fatty liver disease (NAFLD)

and to explore its underlying mechanism.

Methods

Forty-eight 6-week-old female Sprague-Dawley (SD) rats were randomly divided into control group

model group

low-

middle- and high-dose Eq groups [20

40 and 80 mg/(kg·d)]

and estradiol intervention group [E2

0.25 mg/(kg·d)]

with 8 rats in each group. The control group was given sham operation and normal diet. The other groups were given ovariectomy (OVX) combined with high-fat diet. After 16 weeks

the body weight and length of rats were measured

BMI and Lee's index were calculated

and the changes of bone mineral density (BMD) and bone microstructure of rats were detected. After anesthesia

the rats were killed to detect the levels of serum estrogen

TNF-α and IL-6. The liver and femur tissues were observed for morphological changes

and the expression of insulin-like growth factor-1 (IGF-1) in the liver and bone was detected by Western blotting and qRT-PCR.

Results

In the model group

BMI and Lee's index were increased significantly

the liver tissue was obviously injured

the estrogen level and BMD were obvioiusly decreased

and the bone microstructure was seriously damaged

suggesting that postmenopausal NAFLD was complicated with obvious bone injury. The treatment of Eq or E2 increased the level of serum estrogen and BMD

decreased BMI

Lee's index and serum TNF-α and IL-6 levels

attenuated steatosis and lipid accumulation in bone microstructure

and enhanced the expression of IGF-1 at protein and mRNA level in the liver and bone.

Conclusion

Eq can improve the bone injury of postmenopausal NAFLD rats and shows a bone protective effect. The mechanism may partially be related to Eq promoting the expression of IGF-1 and reducing inflammation.

关键词

Keywords

references

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