抗水通道蛋白抗体1和5在原发性干燥综合征的诊断价值

王小宇; 吴红; 王勇; 张利改; 钟兵

doi:10.16016/j.2097-0927.202208201

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抗水通道蛋白抗体1和5在原发性干燥综合征的诊断价值

临床医学 | 更新时间：2026-01-09

- 抗水通道蛋白抗体1和5在原发性干燥综合征的诊断价值
- Diagnostic value of aquaporin 1 and 5 antibodies in primary Sjögren's syndrome
- 陆军军医大学学报 2023年45卷第8期页码：835-841
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)第一附属医院：风湿免疫科
  2.400038 重庆，陆军军医大学(第三军医大学)第一附属医院：检验科
- 作者简介：
  
  [王勇，E-mail：wangyongjhy@126.comWANG Yong, E-mail: wangyongjhy@126.com
- 基金信息：
  
  重庆市科卫联合中医药重点项目(ZY201801010)
- DOI：10.16016/j.2097-0927.202208201
  中图分类号： R442.8;R446.12;R593.2
- 收稿：2022-08-28，
  
  修回：2022-09-11，
  
  纸质出版：2023-04-30
- 稿件说明：
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王小宇, 吴红, 王勇, 等. 抗水通道蛋白抗体1和5在原发性干燥综合征的诊断价值[J]. 陆军军医大学学报, 2023,45(8):835-841.

Xiaoyu WANG, Hong WU, Yong WANG, et al. Diagnostic value of aquaporin 1 and 5 antibodies in primary Sjögren's syndrome[J]. Journal of Army Medical University, 2023, 45(8): 835-841.
王小宇, 吴红, 王勇, 等. 抗水通道蛋白抗体1和5在原发性干燥综合征的诊断价值[J]. 陆军军医大学学报, 2023,45(8):835-841. DOI： 10.16016/j.2097-0927.202208201.

Xiaoyu WANG, Hong WU, Yong WANG, et al. Diagnostic value of aquaporin 1 and 5 antibodies in primary Sjögren's syndrome[J]. Journal of Army Medical University, 2023, 45(8): 835-841. DOI： 10.16016/j.2097-0927.202208201.

摘要

目的

探索抗水通道蛋白抗体1(anti-AQP1)及5(anti-AQP5)在原发性干燥综合征(primary Sjögren’s syndrome，pSS)中的诊断价值及与临床的相关性，寻找新的有助于诊断的潜在生物标记物。

方法

采用横断面研究设计方案，收集2021年9月至2022年3月就诊于陆军军医大学第一附属医院风湿免疫科的pSS患者70例作为观察组，其他结缔组织疾病患者67例及来自体检中心的健康人39例作对照组，采取酶联免疫吸附法(ELISA)检测血清中anti-AQP1和anti-AQP5的水平；同时收集患者的干燥综合征疾病活动指数(EULAR Sjögren’s syndrome disease activity index，ESSDAI)评分、抗核抗体、抗SSA抗体、抗SSB抗体、抗Ro52抗体、ds-DNA、类风湿因子、胞衬蛋白抗体的临床检测数据，应用

检验分析二者相关性。

结果

血清anti-AQP1水平在pSS组及对照组间无统计学差异(

0.05)。pSS组anti-AQP5水平[26.41(24.05，28.08)ng/mL

]

明显高于对照组[7.63(5.49，10.29)ng/mL，

0.05

]

。ROC曲线显示anti-AQP1曲线下面积为0.523(

0.05)，灵敏度为0.371，特异度为0.726。Anti-AQP5曲线下面积为0.915(

0.05)，灵敏度为0.957，特异度为0.821。anti-AQP5与抗SSA抗体、抗SSB抗体、抗Ro52抗体、抗核抗体、类风湿因子之间存在正相关性(

0.05)。anti-AQP5水平与pSS患者的ESSDAI疾病活动度评分之间无相关性(

0.05)。

结论

血清anti-AQP5水平在pSS中明显增加，灵敏度为95.7%，特异度为82.1%，与临床具有一定相关性，具有较高潜在诊断价值。

Abstract

Objective

To explore the diagnostic value and clinical relevance of antibodies against aquaporin-1 (anti-AQP1) and aquaporin-5 (anti-AQP5) in primary Sjögren's syndrome (pSS)

and investigate new potential biomarkers that can contribute to the diagnosis.

Methods

A prospective cohort trial was conducted on 70 patients with pSS (pSS group)

67 patients with other connective tissue diseases and 39 healthy individuals admitted to Department of Rheumatology and Immunology of our hospital from September 2021 to March 2022. The patients with other connective tissue diseases and healthy individuals were assigned into the control group. The contents of anti-AQP1 and anti-AQP5 were measured by enzyme linked immunosorbent assay (ELISA). Meanwhile

EULAR Sjögren's syndrome disease activity index (ESSDAI) score

and levels of anti-nuclear antibody

anti-SSA antibody

anti-SSB antibody

anti-Ro52 antibody

ds- DNA

rheumatoid factor and fodrin were collected and analyzed with

Chi

-square test for correlation.

Results

No statistical difference was observed in the level of anti-AQP1 between the pSS group and control group (

0.05)

but the level of anti-AQP5 was significantly higher in the pSS group than the control group [26.41 (24.05

28.08)

7.63 (5.49

10.29) ng/mL

0.05

]

. ROC curve analysis showed that the area under the curve of anti-AQP1 was 0.523 (

0.05)

with a sensitivity of 0.371 and a specificity of 0.726. While

the area under the curve of anti-AQP5 was 0.915 (

0.05)

with a sensitivity of 0.957 and a specificity of 0.821. The anti-AQP5 level was positively correlated with the levels of anti-SSA antibody

anti-SSB antibody

anti-Ro52 antibody

anti-nuclear antibody and rheumatoid factor (

0.05)

but had no correlation

with ESSDAI score in the pSS patients (

0.05).

Conclusion

The serum level of anti-AQP5 is significantly increased in pSS patients

with a sensitivity of 95.7% and a specificity of 82.1%

and is associated with clinical practice and of high potential diagnostic value.

关键词

Keywords

references

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