柚皮苷改善脂多糖诱导的小鼠抑郁样行为的作用研究

吴川; 孔安琪; 谢汝欣; 李江辉; 蔡云; 范晓棠

doi:10.16016/j.2097-0927.202207029

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柚皮苷改善脂多糖诱导的小鼠抑郁样行为的作用研究

神经科学 | 更新时间：2026-01-09

- 柚皮苷改善脂多糖诱导的小鼠抑郁样行为的作用研究
- Naringin improves lipopolysaccharide-induced depression-like behaviors in mice
- 陆军军医大学学报 2022年44卷第23期页码：2368-2376
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)：医学心理系军事认知心理学教研室
- 作者简介：
  
  [范晓棠，E-mail：fanxiaotang2005@163.comFAN Xiaotang, E-mail: fanxiaotang2005@163.com
- 基金信息：
  
  极端环境医学教育部重点实验室开放课题(KL2019GY003)
- DOI：10.16016/j.2097-0927.202207029
  中图分类号： R-332;R285.5;R749.4
- 收稿：2022-07-06，
  
  修回：2022-09-27，
  
  纸质出版：2022-12-15
- 稿件说明：
移动端阅览
吴川, 孔安琪, 谢汝欣, 等. 柚皮苷改善脂多糖诱导的小鼠抑郁样行为的作用研究[J]. 陆军军医大学学报, 2022,44(23):2368-2376.

Chuan WU, Anqi KONG, Ruxin XIE, et al. Naringin improves lipopolysaccharide-induced depression-like behaviors in mice[J]. Journal of Army Medical University, 2022, 44(23): 2368-2376.
吴川, 孔安琪, 谢汝欣, 等. 柚皮苷改善脂多糖诱导的小鼠抑郁样行为的作用研究[J]. 陆军军医大学学报, 2022,44(23):2368-2376. DOI： 10.16016/j.2097-0927.202207029.

Chuan WU, Anqi KONG, Ruxin XIE, et al. Naringin improves lipopolysaccharide-induced depression-like behaviors in mice[J]. Journal of Army Medical University, 2022, 44(23): 2368-2376. DOI： 10.16016/j.2097-0927.202207029.

摘要

目的

探究柚皮苷(naringin

Nar)对脂多糖(lipopolysaccharide

LPS)诱导的小鼠抑郁样行为的影响。

方法

将30只SPF级，体质量20~23 g的8周龄C57BL/6雄性小鼠分成3组(

=10)：对照组、盐水+脂多糖组(Sal+LPS)、柚皮苷+脂多糖组(Nar+LPS)。后2组用柚皮苷进行预处理(80 mg/kg，腹腔注射，每天1次连续8 d)。用旷场实验、明暗箱实验、高架十字迷宫实验评价小鼠的探索行为和焦虑样行为；用糖水偏好实验、悬尾实验和强迫游泳实验来评价小鼠的抑郁样行为。采用Iba1免疫组化检测Nar和LPS处理对小鼠海马内小胶质细胞数量的影响；应用Western blot检测小鼠海马中NF-κB的蛋白表达水平；qRT-PCR检测小鼠海马内IL-1β、TNF-α和NF-κBmRNA的表达水平。

结果

与对照组相比，Sal+LPS组小鼠糖水偏好降低(

0.05)，强迫游泳不动时间增加(

0.01)，悬尾静止时间增加(

0.05)；海马内小胶质细胞的数量增加(

0.01)；海马内NF-κB蛋白水平及IL-1β、TNF-α、NF-κB mRNA的表达水平增加。与Sal+LPS组小鼠相比，Nar+LPS组小鼠糖水偏好显著

增加(

0.01)，强迫游泳不动时间减少(

0.01)，悬尾静止时间减少(

0.05)；海马内小胶质细胞的数量显著降低(

0.01)；海马内NF-κB蛋白水平及IL-1β、TNF-α、NF-κB mRNA的表达水平显著降低(

0.01)。

结论

柚皮苷可能通过抑制海马内的炎症反应改善脂多糖诱导的小鼠抑郁样行为。

Abstract

Objective

To investigate the effects of naringin (Nar) on lipopolysaccharide (LPS)-induced depression-like behaviors in mice.

Methods

Thirty C57BL/6 male mice (8 weeks old

SPF grade

weighting 20~23 g) were randomly divided into 3 groups (

=10): control group

saline (Sal)+LPS group

and Nar+LPS group. The mice of the last group were pretreated with Nar (80 mg/kg

i.p.

once a day for 8 consecutive days). The exploratory behavior and anxiety-like behavior in mice were assessed by open field test

light and dark box experiment and elevated plus maze test. Sugar water preference test

hanging tail test and forced swimming test were used to evaluate the depression-like behaviors of the mice. Immunohistochemical assay for Iba1 was adopted to detect the effects of Nar and LPS treatments on the number of microglia in the hippocampus of mice. Western blotting and qRT-PCR were performed to determine the protein expression of NF-κB and the mRNA levels of IL-1β

TNF-α and NF-κB in mouse hippocampus

respectively.

Results

As compared with the control group

the mice in the Sal+LPS group showed decreased preference for sugar water (

0.05)

and increased immobile time in the forced swimming test (

0.01) as well as in the hanging tail test (

0.05). The number of microglia in the hippocampus was elevated (

0.01)

the protein level of NF-κB and the mRNA levels of IL-1β

TNF-α and NF-κB were also up-regulated (

0.05). When compared

with the Sal+LPS group

the mice in the Nar+LPS group presented increased preference for sugar water (

0.01)

and decreased immobility time in the forced swimming test (

0.01) and hanging tail test (

0.05)

with higher number of microglia in the hippocampus (

0.01)

and lower protein levels of NF-κB and mRNA levels of IL-1β

TNF-α and NF-κB (

0.01).

Conclusion

Nar may ameliorate LPS-induced depression-like behaviors in mice by inhibiting inflammation in the hippocampus.

关键词

Keywords

references

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