天然小分子HEP-14抗黑色素瘤细胞SK-Mel-103的作用研究

刘芳; 钟沁; 王贤; 韦睿然; 桂黎明

doi:10.16016/j.2097-0927.202206064

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天然小分子HEP-14抗黑色素瘤细胞SK-Mel-103的作用研究

基础医学 | 更新时间：2026-01-09

- 天然小分子HEP-14抗黑色素瘤细胞SK-Mel-103的作用研究
- Anti-melanoma SK-Mel-103 cells effect of a natural small molecule, HEP-14
- 陆军军医大学学报 2022年44卷第23期页码：2392-2400
- 作者机构：
  
  1.550004 贵阳，贵州医科大学基础医学院免疫学教研室
  2.550004 贵阳，贵州医科大学组织工程与干细胞实验中心
  3.230031 合肥，安徽医科大学临床医学院基础医学部
- 作者简介：
  
  [桂黎明，E-mail: guiliming@gmc.edu.cnGUI Liming, E-mail: guiliming@gmc.edu.cn
- 基金信息：
  
  国家自然科学基金委员会-贵州省人民政府联合基金项目(U812403-4);贵州省科技厅社会发展科技支撑计划项目(黔科合支撑[2019]2789号)
- DOI：10.16016/j.2097-0927.202206064
  中图分类号： R285.5;R73-361;R739.5
- 收稿：2022-06-10，
  
  修回：2022-08-12，
  
  纸质出版：2022-12-15
- 稿件说明：
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刘芳, 钟沁, 王贤, 等. 天然小分子HEP-14抗黑色素瘤细胞SK-Mel-103的作用研究[J]. 陆军军医大学学报, 2022,44(23):2392-2400.

Fang LIU, Qin ZHONG, Xian WANG, et al. Anti-melanoma SK-Mel-103 cells effect of a natural small molecule, HEP-14[J]. Journal of Army Medical University, 2022, 44(23): 2392-2400.
刘芳, 钟沁, 王贤, 等. 天然小分子HEP-14抗黑色素瘤细胞SK-Mel-103的作用研究[J]. 陆军军医大学学报, 2022,44(23):2392-2400. DOI： 10.16016/j.2097-0927.202206064.

Fang LIU, Qin ZHONG, Xian WANG, et al. Anti-melanoma SK-Mel-103 cells effect of a natural small molecule, HEP-14[J]. Journal of Army Medical University, 2022, 44(23): 2392-2400. DOI： 10.16016/j.2097-0927.202206064.

摘要

目的

初步探讨天然小分子HEP-14对黑色素瘤SK-Mel-103细胞的抑制作用及相关机制。

方法

用不同浓度的HEP-14(2.5~40 μmol/L)处理黑色素瘤SK-Mel-103细胞后，CCK-8和平板克隆形成实验检测细胞的存活率和克隆的形成，流式细胞术检测细胞周期变化和细胞凋亡，Transwell实验检测细胞迁移能力，RT-qPCR检测CD271和STAT3基因水平的表达，Western blot检测CD271、STAT3、p-STAT3蛋白水平的表达，细胞免疫荧光检测进一步证实CD271在细胞内的表达。

结果

与对照组比较，天然小分子HEP-14能有效抑制SK-Mel-103细胞的生长和克隆的形成(

0.05)，诱导细胞G

/M期阻滞(

0.05)，促进细胞凋亡(

0.05)和抑制细胞的迁移。HEP-14显著抑制SK-Mel-103、SK-Mel-147和SK-Mel-28细胞STAT3的磷酸化(

0.05)，下调CD271的表达(

0.05)。

结论

天然小分子HEP-14能有效抑制黑色素瘤细胞生长并诱导其凋亡；可能通过抑制STAT3的激活下调CD271的表达，从而抑制黑色素瘤细胞的迁移。

Abstract

Objective

To investigate the inhibitory effect of a natural small molecule

HEP-14

on melanoma SK-Mel-103 cells and its underlying mechanism.

Methods

SK-Mel-103 cells were treated with HEP-14 at different concentrations (2.5~40 μmol/L). The untreated cells were used as a control. Cell viability and colony formation were respectively assessed using CCK-8 assay and colony formation assay. Flow cytometry was applied to examine cell cycle and apoptosis. Transwell assay was used to evaluate cellular mobility. Real-time RT-qPCR was used to measure the mRNA expression of CD271 and STAT3

and Western blotting was employed to detect the protein levels of CD271

STAT3 and p-STAT3. Immunofluorescence assay was perform to confirm the expression of CD271.

Results

HEP-14 significantly inhibited the growth and colony formation of SK-Mel-103 cells (

0.05)

induced cell arrest at G2/M phase (

0.05)

promoted cell apoptosis (

0.05) and suppressed cell mobility in comparison with the untreated cells. In addition

HEP-14 treatment suppressed the phosphorylation of STAT3 (

0.05) and down-regulated the expression of CD271 (

0.05) in the melanoma cell lines SK-Mel-103

SK-Mel-147 and SK-Mel-28.

Conclusion

Natural small molecule HEP-14 effectively inhibits the proliferation and induces the apoptosis of melanoma SK-Mel-103 cells

and it suppresses the cell migration by inhibiting activation of STAT to down-regulate CD271 expression.

关键词

Keywords

references

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