小白菊内酯对脓毒症大鼠肠屏障功能的保护作用

郭宁珂; 佘汉; 谭磊; 周远群; 彭小勇; 李涛; 刘良明

doi:10.16016/j.2097-0927.202205042

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小白菊内酯对脓毒症大鼠肠屏障功能的保护作用

专题报道：战创伤休克救治 | 更新时间：2026-01-09

- 小白菊内酯对脓毒症大鼠肠屏障功能的保护作用
- Protective effects of parthenolide on intestinal barrier function in septic rats
- 陆军军医大学学报 2022年44卷第20期页码：2068-2074
- 作者机构：
  
  1.453000 河南新乡，新乡医学院第三附属医院外科
  2.400042 重庆，陆军特色医学中心战伤休克与输血研究室，创伤、烧伤与复合伤国家重点实验室
- 作者简介：
  
  [ 刘良明，E-mail: lmliu62@tmmu.edu.cn LIU Liangming, E-mail: lmliu62@tmmu.edu.cn
- 基金信息：
  
  国家自然科学基金重点项目(81830065)
- DOI：10.16016/j.2097-0927.202205042
  中图分类号： R285.5;R322.45;R631.2
- 收稿：2022-05-10，
  
  修回：2022-07-14，
  
  纸质出版：2022-10-30
- 稿件说明：
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郭宁珂, 佘汉, 谭磊, 等. 小白菊内酯对脓毒症大鼠肠屏障功能的保护作用[J]. 陆军军医大学学报, 2022,44(20):2068-2074.

Ningke GUO, Han SHE, Lei TAN, et al. Protective effects of parthenolide on intestinal barrier function in septic rats[J]. Journal of Army Medical University, 2022, 44(20): 2068-2074.
郭宁珂, 佘汉, 谭磊, 等. 小白菊内酯对脓毒症大鼠肠屏障功能的保护作用[J]. 陆军军医大学学报, 2022,44(20):2068-2074. DOI： 10.16016/j.2097-0927.202205042.

Ningke GUO, Han SHE, Lei TAN, et al. Protective effects of parthenolide on intestinal barrier function in septic rats[J]. Journal of Army Medical University, 2022, 44(20): 2068-2074. DOI： 10.16016/j.2097-0927.202205042.

摘要

目的

探讨小白菊内酯(parthenolide，PTL)对脓毒症大鼠肠屏障功能的保护作用。

方法

采用盲肠结扎穿孔(cecal ligation and puncture，CLP)制作脓毒症大鼠模型，将SD大鼠(雌雄不拘，200±20 g)随机分为6组：正常对照(Normal)组、脓毒症(Sep)组、常规治疗(LR)组(乳酸林格氏液复苏+多巴胺+头孢呋辛钠)和小白菊内酯1、5、10 mg/kg治疗(PTL)组(常规治疗基础上分别给予3种浓度小白菊内酯)。观察各组大鼠动脉血压的变化、存活率和存活时间的变化，确定小白菊内酯治疗脓毒症大鼠的最佳浓度；观察最佳浓度小白菊内酯对脓毒症大鼠肠通透性和肠道病理、血炎症细胞因子水平及肝肾功能的影响。

结果

与正常对照组比较，脓毒症组大鼠存活率与存活时间显著降低，平均动脉压(mean arterial blood pressure，MAP)明显下降，血炎症细胞因子水平升高，肝肾功能损伤；常规治疗可一定程度改善脓毒症大鼠存活率和存活时间，降低血炎症细胞因子水平，改善肝肾功能。1、5、10 mg/kg小白菊内酯组大鼠存活率较常规治疗组分别升高6.3%、43.8%、18.8%，平均动脉血压分别上升1.4%、12.8%、7.1%；其中5 mg/kg组效果最好。小白菊内酯可显著改善脓毒症大鼠肠屏障功能，表现为肠伊文思蓝(Evans blue，EB)含量较常规治疗降低55.1%，血D-乳酸浓度降低69.5%，肠上皮结构改善，上皮细胞坏死减少。小白菊内酯还可显著降低血炎症细胞因子水平，改善脓毒症大鼠肝肾功能。

结论

小白菊内酯对脓毒症大鼠肠屏障功能具有明显的保护作用，其机制可能与抑制脓毒症炎症反应有关。

Abstract

Objective

To investigate the protective effects of parthenolide (PTL) on intestinal barrier function in septic rats.

Methods

The septic model of rats was established by cecal ligation and puncture (CLP)

and SD rats (200±20 g

male/female) were randomly divided into 6 groups: normal control (NC) group

sepsis (Sep) group

conventional treatment (LR) group (lactated Ringer's solution resuscitation+dopamine+cefuroxime sodium)

and parthenolide (PTL) groups (1

5 and 10 mg/kg parthenolide respectively on the basis of LR group). The changes of arterial blood pressure

survival rate and survival time of rats in each group were observed

and the optimal concentration of parthenolide in the treatment was determined. Moreover

the effects of parthenolide (at the optimal concentration) on the intestinal permeability

intestinal pathology

inflammatory cytokine levels

as well as liver and kidney functions in septic rats were further investigated.

Results

As compared with the NC group

the survival rate and survival time of septic rats were significantly decreased

so was the mean arterial pressure (MAP)

while the levels of blood inflammatory cytokines were increased

and the liver and kidney functions were damaged. Conventional treatment improved the survival rate and survival time of septic rats to a certain extent

reduced the levels of inflammation cytokines and alleviated the liver and kidney damages. When compared with the LR group

the survival rates of 1

and 10 mg/kg PTL groups were increased by 6.3%

43.8%

and 18.8%

respectively

and the MAP in the PTL groups was elevated by 1.4%

12.8%

and 7.1%

respectively

with those of 5 mg/kg PTL more significant. Further research found that PTL remarkably ameliorated the intestinal barrier function in septic rats

shown as a 55.1% reduction in the content of intestinal Evans blue (EB)

a 69.5% decline in blood D-lactic acid concentration as compared with the LR group (

0.05)

along with an improvement in intestinal epithelial structure

and a decrease in epithelial cell necrosis. In addition

PTL reduced the inflammatory cytokine levels greatly and improved the liver and kidney functions in septic rats.

Conclusion

PTL shows obvious protective effects on the intestinal barrier function of septic rats

and its mechanism may be related to the inhibition of septic inflammatory response.

关键词

Keywords

references

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