骨髓间充质干细胞外泌体治疗改善高糖腹透液引起的小鼠腹膜纤维化

汪晓月; 蔡青利; 喻芳; 罗佳; 王梨名; 陈佳; 陈客宏; 何娅妮

doi:10.16016/j.2097-0927.202202132

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骨髓间充质干细胞外泌体治疗改善高糖腹透液引起的小鼠腹膜纤维化

基础医学 | 更新时间：2026-01-09

- 骨髓间充质干细胞外泌体治疗改善高糖腹透液引起的小鼠腹膜纤维化
- Exosomes derived from bone marrow mesenchymal stem cells ameliorate peritoneal fibrosis induced by high glucose peritoneal dialysate solution in mice
- 陆军军医大学学报 2022年44卷第22期页码：2291-2299
- 作者机构：
  
  400010 重庆，陆军特色医学中心肾内科
- 作者简介：
  
  [何娅妮，E-mail: heynmail@163.com HE Yani, E-mail: heynmail@163.com
- 基金信息：
- DOI：10.16016/j.2097-0927.202202132
  中图分类号： R329.25;R363.272;R572.2
- 收稿：2021-02-23，
  
  修回：2022-05-25，
  
  纸质出版：2022-11-30
- 稿件说明：
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汪晓月, 蔡青利, 喻芳, 等. 骨髓间充质干细胞外泌体治疗改善高糖腹透液引起的小鼠腹膜纤维化[J]. 陆军军医大学学报, 2022,44(22):2291-2299.

Xiaoyue WANG, Qingli CAI, Fang YU, et al. Exosomes derived from bone marrow mesenchymal stem cells ameliorate peritoneal fibrosis induced by high glucose peritoneal dialysate solution in mice[J]. Journal of Army Medical University, 2022, 44(22): 2291-2299.
汪晓月, 蔡青利, 喻芳, 等. 骨髓间充质干细胞外泌体治疗改善高糖腹透液引起的小鼠腹膜纤维化[J]. 陆军军医大学学报, 2022,44(22):2291-2299. DOI： 10.16016/j.2097-0927.202202132.

Xiaoyue WANG, Qingli CAI, Fang YU, et al. Exosomes derived from bone marrow mesenchymal stem cells ameliorate peritoneal fibrosis induced by high glucose peritoneal dialysate solution in mice[J]. Journal of Army Medical University, 2022, 44(22): 2291-2299. DOI： 10.16016/j.2097-0927.202202132.

摘要

目的

探讨骨髓间充质干细胞(bone marrow mesenchyml stem cell

BMSCs)来源的外泌体在高糖腹透液诱导的小鼠腹膜纤维化中的作用。

方法

选取32只6~8周龄的C57bl/6雄鼠，随机分为为4组(

=8)：正常对照组、生理盐水组、4.25%腹膜透析液组、4.25%腹膜透析液+外泌体组。建立高糖腹透液诱导的腹膜纤维化小鼠模型，42 d后处死小鼠并收集标本。通过改良腹膜平衡实验(peritoneal balance test

PET)检测小鼠腹膜功能；免疫组化法检测小鼠腹膜纤维化标志物的表达；实时定量PCR法检测小鼠腹膜纤维化因子mRNA的表达；ELISA法检测小鼠促纤维化因子的表达。

结果

4.25%腹膜透析液组与对照组小鼠比较，腹膜间皮下区域厚度明显增加(

0.01)；4.25%腹膜透析液组腹膜纤维化标志物(α-SMA、Collagen-Ⅰ、Collagen-Ⅲ)表达显著增强，而4.25%腹膜透析液+外泌体组的腹膜纤维化标志物表达显著低于4.25%腹膜透析液组(

0.01)。4.25%腹膜透析液+外泌体组小鼠相较4.25%腹膜透析液组的超滤功能显著改善(

0.05)。qRT-PCR结果显示，与4.25%腹膜透析液组比较，4.25%腹膜透析液+外泌体组腹膜促纤维化因子TGF-β1(转化生长因子-β1)水平明显降低(

0.05)。

结论

BMSCs来源的外泌体可有效减轻高糖腹透液诱导的小鼠腹膜纤维化。BMSCs来源的外泌体治疗可能成为抑制腹膜纤维化的新方法。

Abstract

Objective

To investigate the role of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) in peritoneal fibrosis induced by high glucose peritoneal dialysis in mice.

Methods

Thirty-two male C57bl/6 mice were randomly divided into 4 groups: the normal control

the saline

the 4.25% peritoneal dialysate and the 4.25% peritoneal dialysate+exosome group. A mouse model of peritoneal fibrosis induced by high glucose peritoneal dialysis was established. The mice were sacrificed after 42 d and the specimens were collected. Peritoneal function was detected by modified peritoneal balance test (PET); The expression of peritoneal fibrosis markers was detected by immunohistochemical method; The expression of peritoneal fibrosis factor mRNA was detected by real-time quantitative PCR

and the expression of pro-fibrotic factor was detected by ELISA.

Results

Compared with the normal control group

the 4.25% peritoneal dialysis group had obviously increased thickness of sub

cutaneous area of peritoneal mesothelium (

0.01). The expression of peritoneal fibrosis markers (α-SMA

collagen-Ⅰ and Collagen-Ⅲ) was significantly increased in the 4.25% peritoneal dialysate group

while the expression of peritoneal fibrosis markers in the 4.25% peritoneal dialysate+exosome group was significantly lower than that in the 4.25% peritoneal dialysate group

0.01). Compared with the 4.25% peritoneal dialysate+exosome group

the ultrafiltration function of mice in the 4.25% peritoneal dialysate+exosome group was significantly improved (

0.05). qRT-PCR results showed that the level of peritoneal fibrosis promoting factor TGF-β1 in the 4.25% peritoneal dialysate+exosome group was significantly lower than that in the 4.25% peritoneal dialysate+exosome group (

0.05).

Conclusion

Exosomes derived from BMSCs can effectively reduce peritoneal fibrosis induced by high glucose peritoneal dialysis solution in mice. Exosomes derived from BMSCs may be a new method to inhibit peritoneal fibrosis.

关键词

Keywords

references

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