胆汁淤积性肝病患者肠道真菌多样性及构成改变

李臻; 杨国栋; 唐波; 唐黎; 雷媛媛; 周安; 刘爽; 张晓英

doi:10.16016/j.2097-0927.202202023

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胆汁淤积性肝病患者肠道真菌多样性及构成改变

临床医学 | 更新时间：2026-01-09

- 胆汁淤积性肝病患者肠道真菌多样性及构成改变
- Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease
- 陆军军医大学学报 2022年44卷第11期页码：1162-1168
- 作者机构：
  
  1.637000 四川南充，川北医学院：基础医学与法医学院病原生物学教研室
  2.400037 重庆，陆军军医大学(第三军医大学)第二附属医院消化内科
  3.637000 四川南充，川北医学院：附属医院消化内科
- 作者简介：
  
  [张晓英，E-mail：zxy_ld2006@163.com ZHANG Xiaoying, E-mail: zxy_ld2006@163.com
- 基金信息：
  
  四川省卫生和计划生育委员会普及应用项目(18PJ386);四川省教育厅科研项目(14ZAO194)
- DOI：10.16016/j.2097-0927.202202023
  中图分类号： R372;R379;R575
- 收稿：2022-02-10，
  
  修回：2022-03-06，
  
  纸质出版：2022-06-15
- 稿件说明：
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李臻, 杨国栋, 唐波, 等. 胆汁淤积性肝病患者肠道真菌多样性及构成改变[J]. 陆军军医大学学报, 2022,44(11):1162-1168.

Zhen LI, Guodong YANG, Bo TANG, et al. Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease[J]. Journal of Army Medical University, 2022, 44(11): 1162-1168.
李臻, 杨国栋, 唐波, 等. 胆汁淤积性肝病患者肠道真菌多样性及构成改变[J]. 陆军军医大学学报, 2022,44(11):1162-1168. DOI： 10.16016/j.2097-0927.202202023.

Zhen LI, Guodong YANG, Bo TANG, et al. Changes in intestinal fungal diversity and compositions in patients with cholestatic liver disease[J]. Journal of Army Medical University, 2022, 44(11): 1162-1168. DOI： 10.16016/j.2097-0927.202202023.

摘要

目的

分析胆汁淤积性肝病患者和健康对照人群的肠道真菌菌群多样性及构成改变。

方法

收集胆汁淤积性肝病患者(

=20)和健康人群对照者(

=22)的新鲜粪便，采用ITS2 DNA测序测定肠道真菌，运用生物信息学方法分析两组间真菌菌群多样性、结构、功能特征之间的差异，以及肠道真菌与临床参数间的相关性。

结果

两组年龄、性别构成、吸烟等均无统计学差异(

0.05)。胆汁淤积性肝病患者组与健康人群对照组相比血清学指标均显著升高(

0.05)；两组间肠道真菌Alpha多样性无显著差异(

0.05)；Beta多样性有显著差异(

0.01)；胆汁淤积性肝病患者组

Myceliophthora

相对丰度增加(

0.01)，

Diutina

相对丰度降低(

0.01)；LEfSe分析结果表明胆汁淤积性肝病患者组中

Myceliophthora

显著富集，且腐生-共生(Saprotroph-Symbiotroph) 菌的丰度显著低于健康人群对照组(

0.05)；Spearman相关系数分析结果显示

Myceliophthora

与ALT(

0.05)、AST、ALP、GGT、TBIL、DBIL(

0.01)均呈正相关。

结论

胆汁淤积性肝病患者与健康人群相比肠道真菌菌群构成有特征性改变，

Myceliophthora

与胆汁淤积性肝病患者疾病严重程度密切相关。

Abstract

Objective

To analyze the changes in intestinal fungi and compositions in patients with cholestatic liver disease from healthy individuals.

Methods

Fresh feces from 20 patients with cholestatic liver disease and 22 healthy individuals were collected. The intestinal fungi were determined by ITS2 DNA sequencing

and the differences of fungal flora diversity

structure and functional characteristics between the 2 groups were analyzed by bioinformatics methods

and the correlation between intestinal fungi and clinical parameters was analyzed.

Results

There were no significant differences in age

sex composition and smoking between the 2 groups (

0.05). The serological indexes were significantly higher in the patients with cholestatic liver disease than the healthy controls (

0.05). No significant difference was seen in the Alpha diversity of intestinal fungi between the 2 groups (

0.05)

but significant differences were observed in Beta diversity (

0.01). The relative abundance of

Myceliophthora

was increased (

0.01) and that of

Diutina

was decreased (

0.01) in the patients with cholestatic liver disease. LEfSe analysis showed that

Myceliophthora

was significantly enriched in the patients

and the abundance of Saprotroph-Symbiotroph was significantly lower in the patients than the healthy controls. Spearman correlation coefficient analysis showed that

Myceliophthora

was positively correlated with alanine transaminase (ALT

0.05)

and aspartate transaminase (AST)

alkaline phosphatase (ALP)

gamma-glutamyl transferase (GGT) and total bilirubin (TBIL) (

0.01).

Conclusion

Characteristic changes are seen in intestinal fungal compositions between the patients with cholestatic liver disease and the healthy controls.

Myceliophthora

is closely associated with the severity of the disease.

关键词

Keywords

references

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