脐带间充质干细胞来源的外泌体对大鼠创伤性胰腺炎损伤的修复

韩丽; 陈思平; 赵治戎; 周世波; 周黎晨; 吉华; 唐政; 谭震; 王涛; 戴睿武

doi:10.16016/j.2097-0927.202201171

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脐带间充质干细胞来源的外泌体对大鼠创伤性胰腺炎损伤的修复

基础医学 | 更新时间：2026-01-09

- 脐带间充质干细胞来源的外泌体对大鼠创伤性胰腺炎损伤的修复
- The repair effect of exosomes derived from human umbilical cord mesenchymal stem cells on traumatic pancreatitis in rats
- 陆军军医大学学报 2022年44卷第16期页码：1642-1650
- 作者机构：
  
  1.610031 成都，西南交通大学医学院临床医学系
  2.610083 成都，西部战区总医院普通外科
- 作者简介：
  
  [戴睿武，E-mail：dairuiwu@swjtu.edu.cnDAI Ruiwu, E-mail: dairuiwu@swjtu.edu.cn
- 基金信息：
  
  西部战区总医院院管课题(2021-XZYG-B16);四川省科技厅重点研发项目(2022YSFS0195)
- DOI：10.16016/j.2097-0927.202201171
  中图分类号： R-332;R329.24;R657.51
- 收稿：2022-01-19，
  
  修回：2022-02-21，
  
  纸质出版：2022-08-30
- 稿件说明：
移动端阅览
韩丽, 陈思平, 赵治戎, 等. 脐带间充质干细胞来源的外泌体对大鼠创伤性胰腺炎损伤的修复[J]. 陆军军医大学学报, 2022,44(16):1642-1650.

Li HAN, Siping CHEN, Zhirong ZHAO, et al. The repair effect of exosomes derived from human umbilical cord mesenchymal stem cells on traumatic pancreatitis in rats[J]. Journal of Army Medical University, 2022, 44(16): 1642-1650.
韩丽, 陈思平, 赵治戎, 等. 脐带间充质干细胞来源的外泌体对大鼠创伤性胰腺炎损伤的修复[J]. 陆军军医大学学报, 2022,44(16):1642-1650. DOI： 10.16016/j.2097-0927.202201171.

Li HAN, Siping CHEN, Zhirong ZHAO, et al. The repair effect of exosomes derived from human umbilical cord mesenchymal stem cells on traumatic pancreatitis in rats[J]. Journal of Army Medical University, 2022, 44(16): 1642-1650. DOI： 10.16016/j.2097-0927.202201171.

摘要

目的

探讨脐带间充质干细胞(human umbilical cord mesenchymal stem cells，hUC-MSCs) 来源的外泌体(hUC-MSCs exosome，hUCMSC-Ex)对创伤性胰腺炎的治疗和保护作用。

方法

60只成年雄性健康SD大鼠(体质量200~250 g)，通过随机区组法分为4组(

=15): 对照组(Control组)，开腹后轻轻翻动胰腺，随后关腹，经尾静脉注射1 mL生理盐水；创伤性胰腺炎组(TP组)，使用造模仪建立创伤性胰腺炎大鼠模型，经尾静脉注射1 mL生理盐水；TP+hUC-MSCs组(造模后经尾静脉注射干细胞)和TP+hUCMSC-Ex组(造模后经尾静脉注射外泌体)。采用超速离心法获取外泌体，使用透射电镜对外泌体进行形态学鉴定；HE染色观察胰腺组织损伤的程度；ELISA法检测淀粉酶、脂肪酶、促炎因子、抗炎因子的表达水平；原位末端标记法(TUNEL)检测细胞凋亡情况；Western blot检测细胞凋亡蛋白Bax、Caspase-3表达水平。

结果

与TP组比较，TP+hUC-MSCs组和TP+hUCMSC-Ex组的胰腺组织病理学评分明显降低(

0.05)，血清淀粉酶、脂肪酶浓度、IL-6、TNF-α表达水平明显降低(

0.05)，而IL-10、TGF-β表达水平明显升高(

0.05)。TP组的凋亡指数明显高于对照组(

0.05)，而TP+

hUC-MSCs组和TP+hUCMSC-Ex组的凋亡指数显著低于TP组(

0.05)，且TP+hUC-MSCs组和TP+hUCMSC-Ex组的凋亡蛋白Bax、Caspase-3表达水平明显降低(

0.05)。

结论

hUCMSC-Ex可以通过控制全身性的炎症反应和抑制大鼠腺泡细胞凋亡，参与胰腺组织再生修复。

Abstract

Objective

To explore the curative and protective effects of exosomes derived from human umbilical cord mesenchymal stem cells(hUCMSC-Ex)on traumatic pancreatitis in rats.

Methods

Sixty grown male SD rats (200~250 g) were selected and randomly divided into four groups (

=15 of each group): control

traumatic pancreatitis (TP)

TP+hUC-MSCs

and TP+hUCMSC-Ex groups. In the control group

the pancreas was gently turned over after laparotomy. Subsequently

the abdomen was closed and 1 mL normal saline was injected via the tail vein. In the TP group

a rat model of TP was established using a model impactor and 1 mL of normal saline was injected

via

the tail vein. hUC-MSCs or hUCMSC-Ex were injected

via

tail vein after modeling in TP+hUC-MSCs and TP+hUCMSC-Ex groups. The ultracentrifugation was used to obtain exosomes

and transmission electron microscopy was used to identify exosomes; HE staining was used for detecting the severity of damage of pancreatic tissue; The expression levels of amylase

lipase

pro-inflammatory factors and anti-inflammatory factors were detected by ELISA; Cell apoptosis was detected by TUNEL; The expression levels of apoptosis proteins Bax and Caspase-3 were detected by Western blotting.

Results

Compared with TP group

TP+hUC-MSCs group and TP+hUCMSC-Ex group had obvious lower score on pancreas histopathological scoring(

0.05); The concentrations of serum amylase and lipase were significantly decreased (

0.05); The expression levels of serum pro-inflammatory factors IL-6 and TNF-α were significant

ly decreased

while the expression levels of anti-inflammatory factors IL-10 and TGF-β were significantly increased (

0.05); The apoptotic index in the TP group was significantly higher than that of in the control group (

0.05)

and the apoptotic index in the TP+hUC-MSCs group and TP+hUCMSC-Ex group was significantly lower than that in the TP group (

0.05). Compared with TP group

the expression levels of pancreatic apoptotic proteins Bax and Caspase-3 were significantly decreased in the TP+hUC-MSCs group and TP+hUCMSC-Ex group (

0.05).

Conclusion

The hUCMSC-Ex can inhibit the apoptosis of rat acinar cells and participate in the regeneration and repair of pancreatic tissue by controlling the systemic inflammatory reaction.

关键词

Keywords

references

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