转录因子SP1上调STMN1促进子宫平滑肌瘤细胞增殖并抑制凋亡

方芳; 潘盛青; 陈培芳; 刘姗姗; 徐彩虹

doi:10.16016/j.2097-0927.202201116

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转录因子SP1上调STMN1促进子宫平滑肌瘤细胞增殖并抑制凋亡

临床医学 | 更新时间：2026-01-09

- 转录因子SP1上调STMN1促进子宫平滑肌瘤细胞增殖并抑制凋亡
- Up-regulation of stathmin by specialized protein 1 promotes proliferation and inhibits apoptosis in uterine leiomyoma cells
- 陆军军医大学学报 2022年44卷第14期页码：1445-1454
- 作者机构：
  
  1.211102 南京，东南大学医学院附属南京同仁医院：妇产科
  2.211102 南京，东南大学医学院附属南京同仁医院：重症医学科
- 作者简介：
  
  [潘盛青，E-mail：pansq@njtrh.orgPAN Shengqing, E-mail: pansq@njtrh.org
- 基金信息：
- DOI：10.16016/j.2097-0927.202201116
  中图分类号： R394.3;R730.23;R737.33
- 收稿：2022-01-13，
  
  修回：2022-03-24，
  
  纸质出版：2022-07-30
- 稿件说明：
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方芳, 潘盛青, 陈培芳, 等. 转录因子SP1上调STMN1促进子宫平滑肌瘤细胞增殖并抑制凋亡[J]. 陆军军医大学学报, 2022,44(14):1445-1454.

Fang FANG, Shengqing PAN, Peifang CHEN, et al. Up-regulation of stathmin by specialized protein 1 promotes proliferation and inhibits apoptosis in uterine leiomyoma cells[J]. Journal of Army Medical University, 2022, 44(14): 1445-1454.
方芳, 潘盛青, 陈培芳, 等. 转录因子SP1上调STMN1促进子宫平滑肌瘤细胞增殖并抑制凋亡[J]. 陆军军医大学学报, 2022,44(14):1445-1454. DOI： 10.16016/j.2097-0927.202201116.

Fang FANG, Shengqing PAN, Peifang CHEN, et al. Up-regulation of stathmin by specialized protein 1 promotes proliferation and inhibits apoptosis in uterine leiomyoma cells[J]. Journal of Army Medical University, 2022, 44(14): 1445-1454. DOI： 10.16016/j.2097-0927.202201116.

摘要

目的

研究微管解聚蛋白1(Stathmin-1

STMN1)受特化蛋白1(specialized protein 1，SP1)转录调控对子宫平滑肌瘤细胞增殖和凋亡的作用。

方法

收集2017年6月至2019年6月在南京同仁医院妇产科接受子宫全切除和次全切除的30例子宫肌瘤患者的肌瘤组织及周围(瘤旁)平滑肌，RT-qPCR检测子宫平滑肌瘤组织和周围平滑肌组织中STMN1和SP1 mRNA水平。CCK-8、TUNEL和Western blot检测敲低STMN1后人子宫平滑肌瘤(human uterine leiomyoma，HuLM)细胞增殖和凋亡。利用JASPAR网站预测转录因子SP1和STMN1启动子的结合位点。采用RT-qPCR、Western blot、荧光素酶、ChIP验证SP1和STMN1的转录调控，进行功能拯救实验。

结果

相较于正常平滑肌细胞，子宫平滑肌瘤组织中STMN1和SP1表达上调(

0.001)。敲低STMN1抑制HuLM细胞增殖并促进凋亡(

0.001)。SP1作为转录因子正向调控STMN1表达水平，敲低SP1能够抑制HuLM细胞增殖并促进凋亡(

0.001)，进一步过表达STMN1逆转敲低SP1对细胞增殖和凋亡的作用(

0.001)。

结论

转录因子SP1通过上调STMN1促进子宫平滑肌瘤细胞增殖并抑制凋亡。

Abstract

Objective

To study the effect of microtubule depolymerization protein 1 (stathmin

STMN1) on the proliferation and apoptosis of uterine leiomyomas cells under the transcriptional regulation of specialized protein 1 (SP1).

Methods

A total of 30 patients of uterine leiomyomas undergoing total and subtotal hysterectomy in our hospital from June 2017 to June 2019 were recruited in this study. The mRNA expression levels of STMN1 and SP1 in the uterine leiomyoma tissues and surrounding smooth muscle tissues were detected with qRT-PCR. After STMN1 was knocked down in human uterine leiomyoma (HuLM) cells

CCK8 assay

TUNEL and Western blotting were used to detect the cell proliferation and apoptosis. JASPAR online tool was employed to predict the binding site of transcription factor SP1 and STMN1 promoter. qRT-PCR

Western blotting

luciferase reporter assay

and chromatin immunoprecipitation (ChIP) were applied to verify the transcriptional regulation of SP1 for STMN1

and functional rescue experiments were also carried out.

Results

The expression levels of STMN1 and SP1 were significantly higher in the uterine leiomyoma tissues than the normal smooth muscle tissues (

0.05). Knockdown of STMN1 inhibited the proliferation and promoted the apoptosis in HuLM cells (

0.001). SP1

as a transcription factor

positively regulated the expression level of STMN1. Knockdown of SP1 could also inhibit the proliferation and promote the apoptosis of HuLM cells (

0.001)

and overexpression of STMN1 reversed the effect of knockdown of SP1 on cell proliferation and apoptosis (

0.001).

Conclusion

The transcription factor SP1 promotes the proliferation and inhibits the apoptosis in the uterine leiomyoma cells by up-regulating STMN1.

关键词

Keywords

references

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