浏览全部资源
扫码关注微信
高糖状态下冠心病患者外周血中辅助性T细胞亚群失衡促进血管内皮细胞损伤
Type 1 T helper /type 2 T helper imbalance accelerates vascular endothelial injury in coronary heart disease patients with hyperglycemia
- 陆军军医大学学报 2022年44卷第6期 页码：576-581
- 作者机构：
250031 济南，解放军联勤保障部队第九六〇医院心血管内科
- 作者简介：
[ 晋群，E-mail: jinqun960yy@163.com JIN Qun, E-mail: jinqun960yy@163.com
- 基金信息：
DOI：10.16016/j.2097-0927.202201034
中图分类号： R322.12;R541.4;R589.1收稿：2022-01-06，
修回：2022-01-29，
纸质出版：2022-03-30
- 稿件说明：
移动端阅览
韩淑芳, 高玉琪, 房洁, 等. 高糖状态下冠心病患者外周血中辅助性T细胞亚群失衡促进血管内皮细胞损伤[J]. 陆军军医大学学报, 2022,44(6):576-581.
Shufang HAN, Yuqi GAO, Jie FANG, et al. Type 1 T helper /type 2 T helper imbalance accelerates vascular endothelial injury in coronary heart disease patients with hyperglycemia[J]. Journal of Army Medical University, 2022, 44(6): 576-581.
韩淑芳, 高玉琪, 房洁, 等. 高糖状态下冠心病患者外周血中辅助性T细胞亚群失衡促进血管内皮细胞损伤[J]. 陆军军医大学学报, 2022,44(6):576-581. DOI： 10.16016/j.2097-0927.202201034.
Shufang HAN, Yuqi GAO, Jie FANG, et al. Type 1 T helper /type 2 T helper imbalance accelerates vascular endothelial injury in coronary heart disease patients with hyperglycemia[J]. Journal of Army Medical University, 2022, 44(6): 576-581. DOI： 10.16016/j.2097-0927.202201034.
摘要
目的
2
观察冠心病(coronary heart disease
CHD)患者不同血糖状态下外周血中1型辅助性T细胞（type 1 T helper cells，Th1）/2型辅助性T细胞（type 2 T helper cells，Th2）的变化，探讨辅助性T细胞在不同血糖状态下的变化对人脐静脉内皮细胞损伤的作用。
方法
2
纳入本院2019年1月至2021年4月临床确诊的冠心病患者73例及同期进行健康体检的志愿者20例（对照组），冠心病患者根据血糖水平分为糖尿病（diabetes mellitus
DM)组25例、糖耐量异常（impaired glucose tolerance group
IGR）组10例和血糖正常（Normal）组38例，测定循环系统外周血中Th1/Th2细胞亚群的百分比及Th1/Th2相关细胞因子的浓度；分析不同细胞亚群对人脐静脉内皮细胞（human umbilical vein endothelial cells，HUVEC）的杀伤作用。
结果
2
DM组冠心病患者外周血中Th1细胞百分比及干扰素γ（interferon- γ
IFNγ）的浓度较其余组显著升高[Th1细胞百分比：DM组（10.13±2.34）%，IGR组（6.38±0.89）%，Normal组（6.13±1.19）%，对照组（6.26±1.18）%，
P
<
0.05；IFNγ：DM组（58.
29±8.24）pg/mL，IGR组(47.60±3.91)pg/mL，Normal组(47.69±5.08)pg/mL，对照组(39.18±4.34) pg/mL，
P
<
0.05
]
，DM组冠心病患者外周血中白介素-10（interleukin-10
IL-10）的浓度较其余组显著降低[DM组: (20.74±3.61)pg/mL，IGR组：(23.83±4.23)pg/mL，Normal组：(30.03±4.28)pg/mL，对照组：(35.69±4.25)pg/mL，
P
<
0.05
]
；DM组冠心病患者的外周血单个核细胞（peripheral blood mononuclear cell
PBMC）对HUVEC的杀伤作用较其余组明显增强[DM组: （67.69±1.96）%，IGR组：（78.19±4.02）%，Normal组：（77.78±2.78）%，对照组：（77.64±4.01）%，
P
<
0.05
]
，而DM组冠心病患者清除CD4
+
T细胞的PBMC对HUVEC的杀伤作用与其余组差异无统计学意义。
结论
2
高糖状态下冠心病患者外周血中Th1/Th2细胞百分比及相关细胞因子水平明显失衡，对血管内皮细胞的损伤作用增强。
Abstract
Objective
2
To observe the changes of type 1 T helper cells (Th1)/type 2 T helper cells (Th2) in peripheral blood of the patients with coronary heart disease (CHD) under different blood glucose conditions
and to investigate the involvement of the changes of Th1/Th2 subsets under different blood glucose conditions on vascular endothelial injury.
Methods
2
Seventy-three CHD patients diagnosed in our hospital from January 2019 to April 2021 and 20 healthy volunteers (control group) were enrolled in this study. According to the blood glucose level of the CHD patients
they were divided into diabetes group (group DM
n
=25)
impaired glucose tolerance group (group IGR
n
=10) and normal blood glucose group (group N
n
=38). The percentages of Th1/Th2 cell subsets and the concentration of Th1/Th2 related cytokines in the peripheral blood were measured. Cytotoxic activity of different cell subsets against human umbilical vein endothelial cells (HUVECs) were evaluated.
Results
2
The percentage of Th1 cells and concentration of interferonγ (IFNγ) were significantly higher in the group DM than the groups IGR
N and Control [percentage of Th1 cells; (10.13±2.34) %
vs
(6.38±0.89)%
(
6.13±1.19)% and (6.26±1.18)%
P
<
0.05; IFNγ: 58.29±8.24
vs
47.60±3.91
47.69±5.08 and 39.18±4.34 pg/mL
P
<
0.05
]
. While the concentration of interleukin-10 (IL-10) in the group DM was obviously lower than that in the other 3 groups (20.74±3.61
vs
23.83±4.23
30.03±4.28 and 35.69±4.25 pg/mL
P
<
0.05). Cytotoxic efficiency of peripheral blood mononuclear cells (PBMC) to HUVEC was significantly elevated in the group DM than the other 3 groups [(67.69±1.96)%
vs
(78.19±4.02)%
(77.78±2.78)% and (77.64±4.01)%
P
<
0.05
]
. But there was no statistical difference in the elimination of CD4
+
T cells in the group DM and the other 3 groups.
Conclusion
2
CHD patients with hyperglycemia present obvious imbalances of Th1/Th2 cells and cytokines in the peripheral blood
which promoting the damage of hyperglycemia to vascular endothelial cells.
关键词
Keywords
references
MA R C W. Epidemiology of diabetes and diabetic complications in China[J]. Diabetologia, 2018, 61(6): 1249-1260. DOI: 10.1007/s00125-018-4557-7.
POZNYAK A, GRECHKO A V, POGGIO P, et al . The diabetes mellitus-atherosclerosis connection: the role of lipid and glucose metabolism and chronic inflammation[J ] . Int J Mol Sci, 2020, 21(5): E1835. DOI: 10.3390/ijms21051835.
GILICZE O, SIMON D, FARKAS N, et al . Characterization of lymphocyte subpopulations and cardiovascular markers in pericardial fluid of cardiac surgery patients[J ] . Clin Hemorheol Microcirc, 2019, 73(4): 579-590. DOI: 10.3233/CH-190594.
VⅡGIMAA M, SACHINIDIS A, TOUMPOURLEKA M, et al . Macrovascular complications of type 2 diabetes mellitus[J ] . Curr Vasc Pharmacol, 2020, 18(2): 110-116. DOI: 10.21 74/1570161117666190405165151.
YUAN T, YANG T, CHEN H, et al . New insights into oxidative stress and inflammation during diabetes mellitus-accelerated atherosclerosis[J ] . Redox Biol, 2019, 20: 247-260. DOI: 10.1016/j.redox.2018.09.025.
SHAO F, ZHENG P L, YU D, et al . Follicular helper T cells in type 1 diabetes[J ] . FASEB J, 2020, 34(1): 30-40. DOI: 10.1096/fj.201901637R.
SAIGUSA R, WINKELS H, LEY K. T cell subsets and functions in atherosclerosis[J]. Nat Rev Cardiol, 2020, 17(7): 387-401. DOI: 10.1038/s41569-020-0352-5.
GISTERÅ A, HANSSON G K. The immunology of atherosclerosis[J]. Nat Rev Nephrol, 2017, 13(6): 368-380. DOI: 10.1038/nrneph.2017.51.
ALI A J, MAKINGS J, LEY K. Regulatory T cell stability and plasticity in atherosclerosis[J]. Cells, 2020, 9(12): 2665. DOI: 10.3390/cells9122665.
ALBANY C J, TREVELIN S C, GIGANTI G, et al . Getting to the heart of the matter: the role of regulatory T- cells (tregs) in cardiovascular disease (CVD) and atherosclerosis[J ] . Front Immunol, 2019, 10: 2795. DOI: 10.3389/fimmu.2019.02795.
HAN S F, LIU P, ZHANG W, et al . The opposite-direction modulation of CD4 + CD25+ Tregs and T helper 1 cells in acute coronary syndromes[J ] . Clin Immunol, 2007, 124(1): 90-97. DOI: 10.1016/j.clim.2007.03.546..
OLSON N C, DOYLE M F, DE BOER I H, et al . Associations of circulating lymphocyte subpopulations with type 2 diabetes: cross-sectional results from the multi-ethnic study of atherosclerosis (MESA)[J ] . PLoS One, 2015, 10(10): e0139962. DOI: 10.1371/journal.pone.0139962.
ZHAO S L, MO Z H, HE H H, et al . Imbalance of T-helper 1/T-helper 2 cytokines and impaired glucose tolerance among patient with acute coronary syndrome[J ] . J Cancer Res Ther, 2018, 14(Supplement): S480-S485. DOI: 10.4103/0973-1482.194346.
ŠIKLOVÁ M, KRAUZOVÁ E, SVOBODOVÁ B, et al . Circulating monocyte and lymphocyte populations in healthy first-degree relatives of type 2 diabetic patients at fasting and during short-term hyperinsulinemia[J ] . Mediators Inflamm, 2019, 2019: 1491083. DOI: 10.1155/2019/1491083.
GIMBRONE M A Jr, GARCÍA-CARDEÑA G. Endothelial cell dysfunction and the pathobiology of atherosclerosis[J]. Circ Res, 2016, 118(4): 620-636. DOI: 10.1161/CIRCRESAHA.115.306301.
CHRYSOHOOU C, KOLLIA N, TOUSOULIS D. The link between depression and atherosclerosis through the pathways of inflammation and endothelium dysfunction[J]. Maturitas, 2018, 109: 1-5. DOI: 10.1016/j.maturitas.2017.12.001.
0
浏览量
0
下载量
0
CSCD
- 网络PDF下载
- Meta-XML下载
- BibTeX下载
- Endnote下载
- RefMan 下载
- NoteExpress下载
- NoteFirst下载
关联资源
相关文章
相关作者
相关机构
- 技术支持由北京北大方正电子有限公司提供 京ICP备09064830号-19
京公网安备11010802024621 - 本系统建议在Chrome、 IE9+ 以上版本浏览器阅读本站内容，360浏览器请切换至极速模式
- Cookies帮助我们提供服务并提供个性化体验。使用本网站，即表示您同意我们使用Cookies
