近红外光响应的二硫化钼/海藻酸钠纳米复合凝胶的制备与止血抗菌性能研究

冉超; 王佳程; 李猛; 顾勋鑫; 郑璐; 李靖

doi:10.16016/j.2097-0927.202201016

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近红外光响应的二硫化钼/海藻酸钠纳米复合凝胶的制备与止血抗菌性能研究

基础医学 | 更新时间：2026-01-09

- 近红外光响应的二硫化钼/海藻酸钠纳米复合凝胶的制备与止血抗菌性能研究
- Preparation of near-infrared light responsive molybdenum disulfide/sodium alginate nanocomposite gel and its hemostatic and antibacterial properties
- 陆军军医大学学报 2022年44卷第8期页码：749-756
- 作者机构：
  
  1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院肝胆外科
  2.400044 重庆，重庆大学能源与动力工程学院，重庆大学-新加坡国立大学新能源材料与器件联合实验室
- 作者简介：
  
  [李靖，E-mail:xqyylj@163.comLI Jing, E-mail: xqyylj@163.com
- 基金信息：
  
  国家自然科学基金项目(52173235);陆军军医大学第二附属医院临床技术重点创新项目(2018JSLC00025)
- DOI：10.16016/j.2097-0927.202201016
  中图分类号： R318.08;R944.15;R973.1
- 收稿：2022-01-04，
  
  修回：2022-02-24，
  
  纸质出版：2022-04-30
- 稿件说明：
移动端阅览
冉超, 王佳程, 李猛, 等. 近红外光响应的二硫化钼/海藻酸钠纳米复合凝胶的制备与止血抗菌性能研究[J]. 陆军军医大学学报, 2022,44(8):749-756.

Chao RAN, Jiacheng WANG, Meng LI, et al. Preparation of near-infrared light responsive molybdenum disulfide/sodium alginate nanocomposite gel and its hemostatic and antibacterial properties[J]. Journal of Army Medical University, 2022, 44(8): 749-756.
冉超, 王佳程, 李猛, 等. 近红外光响应的二硫化钼/海藻酸钠纳米复合凝胶的制备与止血抗菌性能研究[J]. 陆军军医大学学报, 2022,44(8):749-756. DOI： 10.16016/j.2097-0927.202201016.

Chao RAN, Jiacheng WANG, Meng LI, et al. Preparation of near-infrared light responsive molybdenum disulfide/sodium alginate nanocomposite gel and its hemostatic and antibacterial properties[J]. Journal of Army Medical University, 2022, 44(8): 749-756. DOI： 10.16016/j.2097-0927.202201016.

摘要

目的

制备一种新型二硫化钼/海藻酸钠纳米复合凝胶，评价其微观结构、理化特性和止血抗菌性能。

方法

采用两步法制备二硫化钼/海藻酸钠纳米复合凝胶。通过水热法合成二硫化钼(molybdenum disulfide，MoS

)纳米片。制备2%海藻酸钠(sodium alginate，SA)溶液

然后以此为模板，添加不同浓度MoS

分散液，混合均匀后通过钙离子交联得到SA/MoS

-X凝胶；扫描电子显微镜观察SA、SA/MoS

-X微观结构；傅里叶红外光谱测试(FTIR)通过图谱上各种振

动峰的标定分析物质表面官能团；紫外可见光吸收光谱(UV-Vis)测试物质对特定波长的吸收等；光热抗菌实验评价SA/MoS

-X凝胶对大肠杆菌和金黄色葡萄球菌繁殖的抑制作用；大鼠肝脏出血模型评价SA/MoS

-X凝胶的体内止血效果。

结果

扫描电子显微镜结果显示，两步法合成的SA/MoS

-X凝胶的纤维网络呈三维立体多孔结构，其孔隙大小和密度与纯SA凝胶有区别；MoS

附着于SA凝胶表面，均匀分布；紫外可见光吸收光谱显示SA/MoS

-X比SA在808 nm处吸收峰值显著增加。SA/MoS

-X凝胶(分别含1 mg/mL和2 mg/mL MoS

)对大肠杆菌和金黄色葡萄球菌的光热抗菌效果显著高于SA组(

05)。SA/MoS

-X凝胶对SD大鼠肝脏出血模型的止血效果显著高于对照组(

05)。

结论

两步法制备的SA/MoS

-X凝胶具有良好的孔隙率和MoS

结合稳定性，其光热特性和理化性能得到进一步提高，且对大肠杆菌和金黄色葡萄球菌具有较好的抗菌活性，同时具有良好的止血效果。

Abstract

Objective

To prepare a new type of molybdenum disulfide (MoS

)/sodium alginate (SA) nanocomposite gel

and evaluate its microstructure

physical and chemical properties as well as its hemostatic and antibacterial performances.

Methods

The nanocomposite gel was prepared with a two-step method. Firstly

MoS

nanosheets were synthesized by hydrothermal method; secondly

2% SA solution was prepared as a template

being added with different concentrations of MoS

dispersion; and then the SA/MoS

-X gel was obtained by crosslinking with calcium ions after being mixed evenly. The microstructure of SA and SA/MoS

-X were observed by scanning electron microscopy (SEM). Fourier transform infrared spectrometer (FTIR) analysis was used to analyze the surface functional groups of the substance through calibrating various vibration peaks on the spectrum. The absorption of the substance to specific wavelengths were measured by ultraviolet-visible (UV-Vis) light absorption spectroscopy. The inhibitory effects against

E. coli

and

S. aureus

and the hemostatic effect of SA/MoS

-X gel were evaluated by photothermal antibacterial experiment and rat

liver hemorrhage model

respectively.

Results

The SEM results showed that the fiber network of the SA/MoS

-X gel was a 3-D porous structure

with its pore size and density being different from those of pure SA gel

and MoS

was attached to the surface of SA gel and evenly distributed. The UV-Vis absorption spectrum indicated that the absorption peak of SA/MoS

-X was significantly higher than that of SA at 808 nm. The photothermal antibacterial effects against

E.coli

and

S.aureus

were remarkably enhanced in the SA/MoS

-X gel group (containing 1 and 2 mg/mL MoS

respectively) (

0.05). In addition

the hemostatic effect of SA/MoS

-X gel on liver hemorrhage model of SD rats was greatly improved as compared with the control group (

0.05).

Conclusion

The SA/MoS

-X gel prepared by the two-step method has good porosity and binding stability to MoS

with further improved photothermal and physicochemical properties

and presents high antibacterial performance against

E.coli

and

S.aureus

as well as good hemostatic effectiveness.

关键词

Keywords

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