常用麻醉剂对压力超负荷的心衰小鼠心功能检测的影响

唐富琴; 任梅影; 朱雨; 蹇朝; 肖颖彬

doi:10.16016/j.2097-0927.202112266

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常用麻醉剂对压力超负荷的心衰小鼠心功能检测的影响

基础医学 | 更新时间：2026-01-09

- 常用麻醉剂对压力超负荷的心衰小鼠心功能检测的影响
- Effects of anesthetics on cardiac function test in mouse model of pressure overload-induced heart failure
- 陆军军医大学学报 2022年44卷第16期页码：1636-1641
- 作者机构：
  
  400037 重庆，陆军军医大学(第三军医大学)第二附属医院心血管外科，全军心血管外科研究所，血管损伤与修复军队专业实验室
- 作者简介：
  
  [肖颖彬，E-mail: xiaoyb@tmmu.edu.cnXIAO Yingbin, E-mail: xiaoyb@tmmu.edu.cn
- 基金信息：
- DOI：10.16016/j.2097-0927.202112266
  中图分类号： R540.4;R541.6;R614.1
- 收稿：2021-12-30，
  
  修回：2022-04-25，
  
  纸质出版：2022-08-30
- 稿件说明：
移动端阅览
唐富琴, 任梅影, 朱雨, 等. 常用麻醉剂对压力超负荷的心衰小鼠心功能检测的影响[J]. 陆军军医大学学报, 2022,44(16):1636-1641.

Fuqin TANG, Meiying REN, Yu ZHU, et al. Effects of anesthetics on cardiac function test in mouse model of pressure overload-induced heart failure[J]. Journal of Army Medical University, 2022, 44(16): 1636-1641.
唐富琴, 任梅影, 朱雨, 等. 常用麻醉剂对压力超负荷的心衰小鼠心功能检测的影响[J]. 陆军军医大学学报, 2022,44(16):1636-1641. DOI： 10.16016/j.2097-0927.202112266.

Fuqin TANG, Meiying REN, Yu ZHU, et al. Effects of anesthetics on cardiac function test in mouse model of pressure overload-induced heart failure[J]. Journal of Army Medical University, 2022, 44(16): 1636-1641. DOI： 10.16016/j.2097-0927.202112266.

摘要

目的

比较常用麻醉剂对压力超负荷致心衰小鼠心功能检测的影响。

方法

选用8周龄、体质量20~25 g的雄性C57/BL6小鼠120只，通过主动脉弓缩窄构建小鼠心衰模型。实验分为2部分，第一部分共60只小鼠，通过简单随机化法分为4组，分别使用异氟烷、戊巴比妥钠、氯胺酮和三溴乙醇麻醉小鼠，比较不同麻醉药物对心衰小鼠心功能检测的影响；第二部分共60只小鼠，通过简单随机化法分为9组；第1~3组，每组4只，第4~9组，每组8只，分别使用不同浓度的异氟烷和三溴乙醇麻醉心衰小鼠，进一步比较不同浓度麻醉剂对心衰小鼠的麻醉效果。

结果

4种麻醉剂均可能导致心衰小鼠在麻醉诱导阶段的死亡，其中异氟烷导致的心衰小鼠麻醉死亡比例显著低于其他3组(

0.05)；此外，4种麻醉剂均能导致心衰小鼠心功能不同程度的降低，麻醉后清醒时间比正常小鼠长。其中，异氟烷对心衰小鼠心率影响最小，检测心功能指标高于其他麻醉剂组(

0.05)且麻醉后清醒时间短于其他麻醉组(

0.05)。进一步结果显示，麻醉剂浓度越低，其对心脏射血分数(ejection fraction

EF)影响愈小。1%浓度异氟烷麻醉或0.15 mL/10 g三溴乙醇麻醉对小鼠心脏EF值无影响(

0.05)。

结论

4种麻醉剂都会对心衰小鼠的心率和心功能检测造成一定的影响，甚至导致死亡。吸入异氟烷在麻醉心衰小鼠时，其麻醉效果和安全性显著优于其他3种腹腔注射型麻醉剂。

Abstract

Objective

To compare the effects of four common anesthetics on cardiac function test in pressure overload-induced heart failure mice.

Methods

Eight-week-old male C57/BL6 mice (

=120) with a body weight of 20~25 g were selected to establish a mouse model of heart failure by transthoracic aortic coarctation (TAC). Mice were randomly divided into two parts. In the first part

60 mice were randowly divided into four groups (

=15) and anesthetized with four different anesthetics (isoflurane

sodium pentobarbital

ketamine and tribromoethanol)

respectively

to compare the effects of 4 different anesthetics on cardiac function evaluation in mice with heart failure. There were 9 groups in the second part. Each group had 4 mice from the first to the third group

and there were 8 mice in the rest of 3 groups. Mice were anesthetized with different concentrations of isoflurane and tribromoethane to compare the effects of different concentration gradient.

Results

All four anesthetics may lead to the death of mice with heart failure during the anesthesia induction stage

and the proportion of anesthetized death in mice with heart failure caused by isoflurane was significantly lower than that of the other three groups (

0.05). The cardiac function of the mice with heart failure was reduced

and the time of awakening from the anaesthetic was longer than that of the normal mice. Among them

isoflurane had the least effect on the heart rate of mice with heart failure

the ventricular function parameters were higher than the other groups' (

0.05) and the time of awakening from anesthetic was shorter than that of other groups (

0.05). Results showed that the lower concentration of anesthetics have less effect on cardiac EF value. And 1% isoflurane or 0.15 mL/10 g tribromoethanol had no effect on the cardiac EF value.

Conclusion

All four anesthetics could affect the heart rate and cardiac function evaluation of mice with heart failure

and even lead to death. Inhaled isoflurane has significantly better anesthesia effect and safety than other injectable anesthetics in anesthetizing heart failure mice.

关键词

Keywords

references

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