藏汉不同体质指数人群肠道菌群与生化指标的差异研究

罗若城; 洪泽慧; 张保超; 董科; 游兰; 陈静娴; 左浩江; 赵星; 裴晓方

doi:10.16016/j.2097-0927.202112076

您当前的位置：

首页 >

文章列表页 >

藏汉不同体质指数人群肠道菌群与生化指标的差异研究

公共卫生与预防医学 | 更新时间：2026-01-09

- 藏汉不同体质指数人群肠道菌群与生化指标的差异研究
- Differences of gutmicrobiota and serum biochemical markers between Tibetan and Han population with different body mass indexes
- 陆军军医大学学报 2022年44卷第14期页码：1486-封三
- 作者机构：
  
  610041 成都，四川大学华西公共卫生学院/华西第四医院
- 作者简介：
  
  [裴晓方，E-mail：xxpeiscu@163.comPEI Xiaofang, E-mail: xxpeiscu@163.com
- 基金信息：
  
  国家重点研发计划(2017YFC0907305);四川省科技厅应用基础研究项目(2020YJ0233);成都市科技局技术创新研发项目(2019-YF05-01247-SN)
- DOI：10.16016/j.2097-0927.202112076
  中图分类号： R195.1;R372;R446.112
- 收稿：2021-12-09，
  
  修回：2022-02-11，
  
  纸质出版：2022-07-30
- 稿件说明：
移动端阅览
罗若城, 洪泽慧, 张保超, 等. 藏汉不同体质指数人群肠道菌群与生化指标的差异研究[J]. 陆军军医大学学报, 2022,44(14):1486-封三.

Ruocheng LUO, Zehui HONG, Baochao ZHANG, et al. Differences of gutmicrobiota and serum biochemical markers between Tibetan and Han population with different body mass indexes[J]. Journal of Army Medical University, 2022, 44(14): 1486-封三.
罗若城, 洪泽慧, 张保超, 等. 藏汉不同体质指数人群肠道菌群与生化指标的差异研究[J]. 陆军军医大学学报, 2022,44(14):1486-封三. DOI： 10.16016/j.2097-0927.202112076.

Ruocheng LUO, Zehui HONG, Baochao ZHANG, et al. Differences of gutmicrobiota and serum biochemical markers between Tibetan and Han population with different body mass indexes[J]. Journal of Army Medical University, 2022, 44(14): 1486-封三. DOI： 10.16016/j.2097-0927.202112076.

摘要

目的

对比研究四川红原藏族与成都汉族不同体质指数(BMI)人群的肠道菌群及生化指标差异。

方法

于2018年5月至2019年9月在成都青白江、阿坝红原地区收集藏、汉族居民的人口学信息、粪便与血液样本。根据BMI与民族，将研究对象分为藏族肥胖、藏族非肥胖、汉族肥胖与汉族非肥胖等4组，测定肠道菌群及血液生化指标并分析各组差异。

结果

藏族人群甘油三酯(triacylglycerol，TG)水平低于汉族人群，藏、汉族肥胖组总肝固醇(levels of total liver cholesterol，CHOL)、TG、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol，LDL-C)、谷氨酰转移酶(gamma-glutamyl transferase，GGT)、丙氨酸转氨酶(alanine aminotransferase，ALT)、碱性磷酸酶(alkaline phosphatase，ALP)水平均高于非肥胖人群，高密度脂蛋白胆固醇(high-density lipoprotein cholesterol，HDL-C)水平均低于非肥胖人群。藏族人群肠道菌群的物种丰富度与多样性高于汉族人群。汉族肥胖人群梭杆菌门的丰度高于汉族非肥胖人群(

0.05)，而厚壁菌门的丰度低于汉族非肥胖人群(

0.05)；藏族肥胖人群疣微菌门的丰度低于藏族非肥胖人群(

0.05)。属水平上，藏族人群共有12个菌属的丰度高于汉族人群(

0.05)，而汉族人群共有6个菌属的丰度高于藏族人群(

0.05)。其中，在藏族人群中丰度较高的柯林斯氏菌属与TG、LDL-C、GGT、ALT、AST呈正相关，与HDL-C呈负相关(|

|＞0，

0.05)；汉族人群中丰度较高的梭杆菌属与TG、GGT呈正相关，与HDL-C呈负相关(|

|＞0，

0.05)。

结论

藏汉不同BMI人群的肠道菌群结构和生化指标差异明显，柯林斯氏菌属、梭杆菌属可能分别与藏族和汉族人群的肥胖有关。

Abstract

Objective

To compare and analyze the differences in the gut microbiota and serum biochemical markers between the Tibetan people in Hongyuan

and Han people in Chengdu

Sichuan Province with different body mass indexes (BMI).

Methods

From May 2018 to September 2019

Tibetan and Han residents from Aba Hongyuan area and Chengdu Qingbaijiang area were recruited based on the China multi-ethnic cohort (CMEC). Their demographic information

height

body mass

stool and blood samples were collected. According to BMI and ethnic groups

the subjects were subsequently divided into obese and non-obese Tibetans

as well as corresponding Han groups. The intestinal microflora and blood biochemical markers were measured

and the differences among groups were compared and analyzed.

Results

The triacylglycerol (TG) level in Tibetan population was inferior to that in Han generally. The levels of total liver cholesterol (CHOL)

low-density lipoprotein cholesterol (LDL-C)

gamma-glutamyl transferase (GGT)

alanine aminotransferase (ALT)

and alkaline phosphatase (ALP) were higher

while the level of high-density lipoprotein cholesterol (HDL-C) was lower in the obese population than the non-obese population. The species richness and diversity of gut microbiota were greater in Tibetans than in Han population. The abundance of

fusobacterium

in the obese Han group was more than that in the non-obese Han (

0.05)

whereas that of

firmicutes

showed the opposite (

0.05). The abundance of

verrucomicrobia

in the obese Tibetans was lower than that in the non-obese Tibetans (

0.05). At the genus level

the Tibetan group had 12 genera of higher abundance than Han population (

0.05)

while the abundance of 6 genera in the Han group was greater than that in Tibetan population (

0.05). Among them

collinsella

genus with superior abundance in the Tibetan population was positively correlated with TG

LDL-C

GGT

ALT

and aspartate aminotransferase (AST)

and negatively correlated with HDL-C (|

0.05). The

fusobacterium

genus

which was more abundant in Han population

was posit

ively correlated with TG and GGT

and negatively correlated with HDL-C (|

0.05).

Conclusion

The gut microbiota structure and biochemical indexes vary considerably among Tibetan and Han population with different BMI.

collinsella

and

fusobacterium

may be related to the obesity of Tibetans and Han population

respectively.

关键词

Keywords

references

BÄCKHED F, DING H, WANG T, et al . The gut microbiota as an environmental factor that regulates fat storage[J ] . Proc Natl Acad Sci USA, 2004, 101(44): 15718-15723. DOI: 10.1073/pnas.0407076101.

CAESAR R, FÅK F, BÄCKHED F. Effects of gut microbiota on obesity and atherosclerosis via modulation of inflammation and lipid metabolism[J]. J Intern Med, 2010, 268(4): 320-328. DOI: 10.1111/j.1365-2796.2010.02270.x.

BOULANGÉ C L, NEVES A L, CHILLOUX J, et al . Impact of the gut microbiota on inflammation, obesity, and metabolic disease[J ] . Genome Med, 2016, 8(1): 42. DOI: 10.1186/s13073-016-0303-2.

NICOLUCCI A C, HUME M P, MARTÍNEZ I, et al . Prebiotics reduce body fat and alter intestinal microbiota in children who are overweight or with obesity[J ] . Gastroenterology, 2017, 153(3): 711-722. DOI: 10.1053/j.gastro.2017.05.055.

LIU W J, ZHANG J C, WU C Y, et al . Unique features of ethnic Mongolian gut microbiome revealed by metagenomic analysis[J ] . Sci Rep, 2016, 6: 34826. DOI: 10.1038/srep34826.

LE CHATELIER E, NIELSEN T, QIN JJ, et al . Richness of human gut microbiome correlates with metabolic markers[J ] . Nature, 2013, 500(7464): 541-546. DOI: 10.1038/nature12506.

ZHAO X, HONG F, YIN J Z, et al . Cohort profile: the China multi-ethnic cohort (CMEC)study[J ] . Int J Epidemiol, 2021, 50(3): 721-721l. DOI: 10.1093/ije/dyaa185.

中华人民共和国卫生部疾病控制司. 中国成人超重和肥胖症预防控制指南[M]. 北京: 人民卫生出版社, 2006.

Department of Disease Control, Ministry of Health of P. R, China. Guidelines for prevention and control of overweight and obesity in Chinese adults[M]. Beijing: People's Medical Publishing House, 2006.

WEI Y J, REN T Q, ZHANG L. Dix-seq: an integrated pipeline for fast amplicon data analysis[J]. bioRxiv, 2020, DOI: 10.1101/2020.05.11.089748.

SIMPSON G, SOLYMOS P, STEVENS M, et al . Vegan: community ecologypackage[J ] . Time International, 2010.

DONG K, WU K P, ZHENG T L, et al . Comparative study of oral bacteria and fungimicrobiota in Tibetan and Chinese Han living at different altitude[J ] . Tohoku J Exp Med, 2021, 254(2): 129-139. DOI: 10.1620/tjem.254.129.

ZUO H J, FU M R, ZHAO H L, et al . Study on the salivary microbial alteration of menwith head and neck cancer and its relationship with symptoms in southwest China[J ] . Front Cell Infect Microbiol, 2020, 10: 514943. DOI: 10.3389/fcimb.2020.514943.

PARADIS E, CLAUDE J, STRIMMER K. APE: analyses of phylogenetics and evolution in R language[J]. Bioinformatics, 2004, 20(2): 289-290. DOI: 10.1093/bioinformatics/btg412.

FERNANDES A D, MACKLAIM J M, LINN T G, et al . ANOVA-like differential expression (ALDEx) analysis for mixed population RNA-Seq[J ] . PLoS One, 2013, 8(7): e67019. DOI: 10.1371/journal.pone.0067019.

GOMES A C, HOFFMANN C, MOTA J F. The human gutmicrobiota: metabolism and perspective in obesity[J]. Gut Microbes, 2018, 9(4): 308-325. DOI: 10.1080/19490976.2018.1465157.

GÉRARD P. Gut microbiota and obesity[J]. Cell Mol Life Sci, 2016, 73(1): 147-162. DOI: 10.1007/s00018-015-2061-5.

LI K, DAN Z, GESANG L B, et al . Comparative analysis of gut microbiota of native Tibetan and Han populations living at different altitudes[J ] . PLoS One, 2016, 11(5): e0155863. DOI: 10.1371/journal.pone.0155863.

武雅婷, 肖颖, 范紫玮, 等. 藏汉不同骨密度人群肠道菌群及粪便短链脂肪酸差异研究[J]. 现代预防医学, 2021, 48(22): 9.

WU Y T, XIAO Y, FAN Z W, et al . Differences of intestinal microflora and fecal short-chain fatty acids between Tibetan and Han populations with different bone densities[J ] . Modern Preventive Med, 2021, 48(22): 9.

刘怡萱, 曹鹏熙, 马红梅, 等. 健康藏族与汉族大学生肠道细菌多样性研究[J]. 微生物学报, 2019, 59(9): 11. DOI: 10.13343/j.cnki.wsxb.20190013.

LIU Y X, CAO P X, MA H M, et al . Diversity of intestinal bacteria in healthy Tibetan and Han college students[J ] . Acta Microbiologica Sinica, 2019, 59(9): 11. DOI: 10.13343/j.cnki.wsxb.20190013.

梁田, 马利锋, 张致英, 等. 海拔高度与藏族人群肠道菌群的宏基因组学关联分析[J]. 天津师范大学学报: 自然科学版, 2021. DOI: 0.19638/j.issn1671-1114.20210207.

LIANG T, MA L F, ZHANG Z Y, et al . Metagenomic association analysis between altitude and intestinal microflora in Tibetan population[J ] . J Tianjin Normal Univ: Natural Sci, 2021. DOI: 0.19638/j.issn1671-1114.20210207.

ZHANG H S, DIBAISE J K, ZUCCOLO A, et al . Human gut microbiota in obesity and after gastric bypass[J ] . Proc Natl Acad Sci USA, 2009, 106(7): 2365-2370. DOI: 10.1073/pnas.0812600106.

VAZQUEZ-MORENO M, PEREZ-HERRERA A, LOCIA-MORALES D, et al . Association of gut microbiome with fasting triglycerides, fasting insulin and obesity status in Mexican children[J ] . Pediatr Obes, 2021, 16(5): e12748. DOI: 10.1111/ijpo.12748.

LAHTI L, SALONEN A, KEKKONEN R A, et al . Associations between the human intestinalmicrobiota, Lactobacillus rhamnosus GG and serum lipids indicated by integrated analysis of high-throughput profiling data[J ] . PeerJ, 2013, 1: e32. DOI: 10.7717/peerj.32.

CHEN J, WRIGHT K, DAVIS J M, et al . An expansion of rare lineage intestinal microbes characterizes rheumatoidarthritis[J ] . Genome Med, 2016, 8(1): 43. DOI: 10.1186/s13073-016-0299-7.

DERRIEN M, ADAWI D, AHRNÉ S, et al . The intestinal mucosa as a habitat of the gut microbiota and a rational target for probiotic functionality and safety[J ] . Microb Ecol Heal Dis, 2004, 16(2/3): 137-144. DOI: 10.3402/mehd.v16i2-3.7934.

DE LA CUESTA-ZULUAGA J, CORRALES-AGUDELO V, CARMONA J A, et al . Body size phenotypes comprehensively assess cardiometabolic risk and refine the association between obesity and gut microbiota[J ] . Int J Obes (Lond), 2018, 42(3): 424-432. DOI: 10.1038/ijo.2017.281.

NADERPOOR N, MOUSA A Y, GOMEZ-ARANGO L F, et al . Faecal microbiota are related to insulin sensitivity and secretion in overweight or obese adults[J ] . J Clin Med, 2019, 8(4): 452. DOI: 10.3390/jcm8040452.

ZHOU Q, ZHANG Y F, WANG X X, et al . Gut bacteria Akkermansia is associated with reduced risk of obesity: evidence from the American Gut Project[J ] . Nutr Metab (Lond), 2020, 17: 90. DOI: 10.1186/s12986-020-00516-1.

BASHIR A, MISKEEN A Y, HAZARI Y M, et al . Fusobacterium nucleatum, inflammation, and immunity: the fire within human gut[J ] . Tumour Biol, 2016, 37(3): 2805-2810. DOI: 10.1007/s13277-015-4724-0.

CROVESY L, MASTERSON D, ROSADO E L. Profile of the gut microbiota of adults with obesity: a systematic review[J]. Eur J Clin Nutr, 2020, 74(9): 1251-1262. DOI: 10.1038/s41430-020-0607-6.

GAO X F, ZHANG M R, XUE J M, et al . Body mass index differences in the gut microbiota are gender specific[J ] . Front Microbiol, 2018, 9: 1250. DOI: 10.3389/fmicb.2018.01250.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

小檗碱调节肠道菌群改善小鼠药物性肝损伤

胆囊切除术与肠道菌群组成及其功能变化关系的研究

基于多中心数据探索肠道菌群对结直肠息肉的预测作用

基于体成分数据分析中老年超重/肥胖女性肠道菌群的特征

胆汁淤积性肝病患者肠道菌群特征及其临床相关性研究