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信号分子AI-2通过密度感应系统促进铜绿假单胞菌生物被膜形成和毒力因子产生
Signal molecule autoinducer 2 promotes biofilm formation and virulence production in
Pseudomonas aeruginosa by quorum sensing system- 陆军军医大学学报 2022年44卷第14期 页码：1440-1444
- 作者机构：
1.400014 重庆，重庆医科大学附属儿童医院：急诊科，国家儿童健康与疾病临床医学研究中心，儿童发育疾病研究教育部重点实验室，儿科学重庆市重点实验室
2.400014 重庆，重庆医科大学附属儿童医院：新生儿研究室，国家儿童健康与疾病临床医学研究中心，儿童发育疾病研究教育部重点实验室，儿科学重庆市重点实验室
3.400020 重庆，重庆市江北区人民医院药剂科
- 作者简介：
[余加林，E-mail: yujialin468@163.comYU Jialin, E-mail: yujialin468@163.com
[谭利平, E-mail: tanlp0825@hotmail.comTAN Liping, E-mail: tanlp0825@hotmail.com
- 基金信息：重庆市自然科学基金(cstc2020jcyj-msxmX0253)
DOI：10.16016/j.2097-0927.202112073
中图分类号： R378.99;R394.2收稿：2021-12-09，
修回：2022-04-01，
纸质出版：2022-07-30
- 稿件说明：
移动端阅览
李洪东, 余加林, 谭利平, 等. 信号分子AI-2通过密度感应系统促进铜绿假单胞菌生物被膜形成和毒力因子产生[J]. 陆军军医大学学报, 2022,44(14):1440-1444.
Hongdong LI, Jialin YU, Liping TAN, et al. Signal molecule autoinducer 2 promotes biofilm formation and virulence production in
Pseudomonas aeruginosa by quorum sensing system[J]. Journal of Army Medical University, 2022, 44(14): 1440-1444.李洪东, 余加林, 谭利平, 等. 信号分子AI-2通过密度感应系统促进铜绿假单胞菌生物被膜形成和毒力因子产生[J]. 陆军军医大学学报, 2022,44(14):1440-1444. DOI： 10.16016/j.2097-0927.202112073.
Hongdong LI, Jialin YU, Liping TAN, et al. Signal molecule autoinducer 2 promotes biofilm formation and virulence production in
Pseudomonas aeruginosa by quorum sensing system[J]. Journal of Army Medical University, 2022, 44(14): 1440-1444. DOI： 10.16016/j.2097-0927.202112073.
摘要
目的
2
研究信号分子AI-2对铜绿假单胞菌(
P
.
aeruginosa
P
.
a
)野生株(PAO1)及其密度感应(quorum sensing
QS)系统基因
lasR
/
rhlR
缺失株(
ΔlasRΔrhlR
)生物被膜(biofilm，BF)形成和毒力因子产生的影响。
方法
2
以PAO1和PAO1
LasR
rhlR
QS基因敲除菌株(
P
.
a
ΔLasR
Δ
rhlR
)建立生物被膜模型后分4组进行实验：单独PAO1组、AI-2+PAO1组、单独
P
.
a
ΔLasR
Δ
rhlR
组、AI-2+
P
.
a
ΔLasR
Δ
rhlR
组。结晶紫染色测量各组细菌生物被膜量；激光共聚焦显微镜观察BF形成；K-B纸片扩散法行药敏实验；氯仿萃取法、刚果红法分别测定不同组产生毒力因子绿脓菌素和弹性蛋白酶的量或者活性。
结果
2
AI-2的作用后，AI-2+PAO1组生物被膜形成、毒力因子的产生都显著高于单独PAO1组，抗生素抑菌圈直径显著小于单独PAO1组(
P
<
0.05)。
P
.
a
ΔLasR
Δ
rhlR
组生物被膜形成、毒力因子的产生都显著低于PAO1组，抗生素抑菌圈直径显著大于PAO1组(
P
<
0.05)。而单独
P
.
a
ΔLasR
Δ
rhlR
与AI-2+
P
.
a
ΔLasR
Δ
rhlR
两组相比，其生物被膜形成、毒力因子的产生和抗生素抑菌圈直径无明显差异(
P
>
0.05)。
结论
2
AI-2通过
P
.
a
QS系统表达促进其BF形成、毒力因子的产生。
Abstract
Objective
2
To investigate the effects of signal molecule autoinducer 2 (AI-2) on the biofilm formation and virulence production in the wild type strain
PAO1 and the strain with deficient quorum sensing genes
lasR
and
rhlR
(ΔlasRΔ
rhlR
) of
Pseudomonas
aeruginosa
(
P
.
a
).
Methods
2
The wild strain PAO1 and the
P
.
a
ΔLasR
Δ
rhlR
strain were used to establish biofilm model. The following experiments were performed on 4 groups
including PAO1 group
AI-2+PAO1 group
P
.
a
ΔLasR
Δ
rhlR
group and AI-2+
P
.
a
ΔLasR
Δ
rhlR
group. The biofilm formation was measured by crystal violet assay and observed with confocal laser scanning microscopy (CLSM). Kirby-Bauer disk diffusion was used for drug sensitivity test. Virulence factor pyocyanin and elastase production were measured by chloroform extraction method and Congo red assay
respectively.
Results
2
Compared to the wild type PAO1
the biofilm formation and virulence production were significantly increased in AI-2+PAO1 group
as well as the diameter of inhibition zone was significantly decreased(
P
<
0.05)
the biofilm formation and virulence production were significantly reduced in AI-2+
P
.
a
ΔLasR
Δ
rhlR
group
and the diameter of inhibition zone was significantly enlarged (
P
<
0.05). However
there were no significant differences between the
P
.
a
ΔLasR
Δ
rhlR
group and AI-2+
P
.
a
ΔLasR
Δ
rhlR
group in biofilm formation
virulence production and the diameter of inhibition zone (
P
>
0.05).
Conclusion
2
AI-2 enhances the biofilm formation and virulence production of
P
.
a
by the quorum sensing system.
关键词
Keywords
references
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