大黄素抑制MRSA生物膜活性及其作用机制研究

刘婧怡; 蔡仑; 段浩; 刘畅; 李海波; 刘明

doi:10.16016/j.2097-0927.202112068

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大黄素抑制MRSA生物膜活性及其作用机制研究

药学 | 更新时间：2026-01-09

- 大黄素抑制MRSA生物膜活性及其作用机制研究
- Inhibitory effect of emodin on MRSA biofilm and its mechanism
- 陆军军医大学学报 2022年44卷第7期页码：684-690
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)药学与检验医学系: 微生物与生化药学教研室，国家免疫生物制品工程技术研究中心
  2.400038 重庆，陆军军医大学(第三军医大学)药学与检验医学系: 分析化学教研室
  3.154007 黑龙江佳木斯，佳木斯大学临床医学院
- 作者简介：
  
  [李海波, E-mail: lihaibo@tmmu.edu.cn LI Haibo, E-mail: lihaibo@tmmu.edu.cn
  [刘明, E-mail: lm1859@163.com LIU Ming, E-mail: lm1859@163.com
- 基金信息：
  
  重庆市自然科学基金重点项目(cstc2020jcyj-zdxmX0027);国家自然科学基金青年科学基金(81603164)
- DOI：10.16016/j.2097-0927.202112068
  中图分类号： R382.71;R285.5;R378.11
- 收稿：2021-12-08，
  
  修回：2022-01-24，
  
  纸质出版：2022-04-15
- 稿件说明：
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刘婧怡, 蔡仑, 段浩, 等. 大黄素抑制MRSA生物膜活性及其作用机制研究[J]. 陆军军医大学学报, 2022,44(7):684-690.

Jingyi LIU, Lun CAI, Hao DUAN, et al. Inhibitory effect of emodin on MRSA biofilm and its mechanism[J]. Journal of Army Medical University, 2022, 44(7): 684-690.
刘婧怡, 蔡仑, 段浩, 等. 大黄素抑制MRSA生物膜活性及其作用机制研究[J]. 陆军军医大学学报, 2022,44(7):684-690. DOI： 10.16016/j.2097-0927.202112068.

Jingyi LIU, Lun CAI, Hao DUAN, et al. Inhibitory effect of emodin on MRSA biofilm and its mechanism[J]. Journal of Army Medical University, 2022, 44(7): 684-690. DOI： 10.16016/j.2097-0927.202112068.

摘要

目的

探究大黄素抗耐甲氧西林金黄色葡萄球菌(methicillin-resistant

Staphylococcus aureus

MRSA)生物膜作用及其机制。

方法

微量肉汤稀释法测定大黄素对MRSA标准株及5种不同地区临床分离株的最低抑菌浓度(minimum inhibitory concentration

MIC)和最低杀菌浓度(minimum bactericidal concentration

MBC)，绘制抑菌曲线评估其动态抑菌效果；结晶紫染色测定大黄素抑制MRSA生物膜的作用，扫描电镜和激光共聚焦显微镜观察其对MRSA生物膜形态的影响；荧光定量PCR检测ica操纵子基因的表达。

结果

大黄素对不同MRSA菌株的MIC为2~8 μg/mL，MBC为4~16 μg/mL；大黄素可显著抑制MRSA生物膜的形成，并呈剂量依赖关系；大黄素对生物膜结构具有破坏作用，且显著增加了生物膜内死细菌的比例；荧光定量PCR结果提示大黄素显著下调了ica操纵子基因的表达。

结论

大黄素具有抑制MRSA生物膜的作用，并可能通过下调ica操纵子基因的表达抑制细菌生物膜的形成。

Abstract

Objective

To investigate anti-biofilm activity and mechanisms of emodin against methicillin-resistant

Staphylococcus aureus

(MRSA)

in vitro

Methods

Microbroth dilution was explored to determine minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of emodin against MRSA252 strain and clinical isolations. Inhibitory curve were draw to estimate dynamic bacteriostatic effect. Crystal violet staining was used to investigate the inhibitory effect of emodin against MRSA biofilm. The effect of emodin on the morphology of MRSA biofilm was observed by scanning electron microscopy (SEM) and laser scanning confocal microscopy (LSCM). The expression of intercellular adhesion (ica) operon was detected by fluorescence quantitative PCR.

Results

The MICs of emodin against different strains of MRSA ranged from 2 to 8 μg/mL

while MBCs were from 4 to 16 μg/mL. Emodin obviously inhibited the formation of biofilm in a dose-dependent manner. Besides

it destroyed biofilm structure and increased the ratio of dead bacteria under biofilm. Fluorescence quantitative PCR showed that emodin significantly down-regulated the expression of ica operon.

Conclusion

Emodin can inhibit MRSA biofilm

in vitro

and may restrain the formation of bacterial biofilm by down-regulating the expression of ica operon.

关键词

Keywords

references

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