两种方法制备自体富血小板血浆的质量及安全性比较分析

宋娜丽; 王世春; 易中梅; 张彬; 刘奇; 李素梅; 范娅涵; 姚春艳

doi:10.16016/j.2097-0927.202110184

您当前的位置：

首页 >

文章列表页 >

两种方法制备自体富血小板血浆的质量及安全性比较分析

临床医学 | 更新时间：2026-01-09

- 两种方法制备自体富血小板血浆的质量及安全性比较分析
- Comparative analysis on quality and safety of autologous platelet-rich plasma collected and prepared by 2 methods
- 陆军军医大学学报 2022年44卷第6期页码：571-575
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)第一附属医院: 输血科，中国人民解放军重庆血站
  2.404300 重庆，重庆市忠县人民医院输血科
- 作者简介：
  
  [ 姚春艳，E-mail: yaochunyan@tmmu.edu.cn YAO Chunyan, E-mail: yaochunyan@tmmu.edu.cn
- 基金信息：
- DOI：10.16016/j.2097-0927.202110184
  中图分类号： R457.14
- 收稿：2021-10-28，
  
  修回：2021-12-03，
  
  纸质出版：2022-03-30
- 稿件说明：
移动端阅览
宋娜丽, 王世春, 易中梅, 等. 两种方法制备自体富血小板血浆的质量及安全性比较分析[J]. 陆军军医大学学报, 2022,44(6):571-575.

Nali SONG, Shichun WANG, Zhongmei YI, et al. Comparative analysis on quality and safety of autologous platelet-rich plasma collected and prepared by 2 methods[J]. Journal of Army Medical University, 2022, 44(6): 571-575.
宋娜丽, 王世春, 易中梅, 等. 两种方法制备自体富血小板血浆的质量及安全性比较分析[J]. 陆军军医大学学报, 2022,44(6):571-575. DOI： 10.16016/j.2097-0927.202110184.

Nali SONG, Shichun WANG, Zhongmei YI, et al. Comparative analysis on quality and safety of autologous platelet-rich plasma collected and prepared by 2 methods[J]. Journal of Army Medical University, 2022, 44(6): 571-575. DOI： 10.16016/j.2097-0927.202110184.

摘要

目的

比较血袋法与单采法制备富血小板血浆(platelet-rich plasma

PRP)的质量及安全性。

方法

回顾性分析2019年7月至2021年8月在陆军军医大学第一附属医院输血科自体PRP采集的患者66例，根据制备方式分为血袋组和单采组，其中血袋组30例制备47次，单采组36例制备36次。比较2组PRP产品中红细胞、白细胞、血小板及产品倍数。收集患者有无不良反应的记录，并进行安全性评价。

结果

单采组制备时间、产品容量、产品浓度及产品倍数均优于血袋组，差异有统计学意义(

0.01)；红细胞混入量2组比较差异有统计学意义(

0.01)，血袋制备组少于单采制备组；白细胞混入量2组差异无统计学意义。两组患者均无不良反应。

结论

单采组PRP产品血小板浓度高于血袋组且用时更短，推荐单采制备为首选，血袋制备为备选。

Abstract

Objective

To compare the quality and safety of platelet-rich plasma (PRP) by blood bag and monopheresis.

Methods

A retrospective analysis was performed on 66 patients treated with autologous PRP therapy in the blood transfusion department of First Affiliated Hospital of Army Medical University from July 2019 to August 2021. According to preparation procedure

they were divided into blood bag group (

=30

with 47 times of preparation) and monopheresis group (

=36

with 36 times of preparation). The counts of erythrocytes

leukocytes and platelets and product multiples in the PRP products were detected and compared between the 2 groups. Adverse reactions were recorded and safety was evaluated.

Results

The preparation time

product volume

product concentration and product multiple were significantly better in the monopheresis group than the blood bag group (

0.01). The amount of mixed red blood cells was statistically less in the blood bag

group than the apheresis group (

0.01). There was no notable difference in the amount of mixed white blood cells between the 2 groups. No adverse reactions were seen in both groups.

Conclusion

The monopheresis PRP products have higher platelets concentration than those by blood bag and need shorter time of procedure group. Therefore

monopheresis is recommended as first choice and blood bag as the alternative.

关键词

Keywords

references

KAUSHIK A, KUMARAN M S. Platelet-rich plasma: the journey so far![J]. Indian Dermatol Online J, 2020, 11(5): 685-692. DOI: 10.4103/idoj.IDOJ_369_19.

GENTILE P, GARCOVICH S. Systematic review—the potential implications of different platelet-rich plasma (PRP) concentrations in regenerative medicine for tissue repair[J]. Int J Mol Sci, 2020, 21(16): E5702. DOI: 10.3390/ijms21165702.

GUPTA V, PARIHARA S, PATHAK M, et al . Comparison of platelet-rich plasma prepared using two methods: manual double spin method versus a commercially available automated device[J ] . Indian Dermatol Online J, 2020, 11(4): 575-579. DOI: 10.4103/idoj.idoj_653_19.

SRINIVASAN V R, REKHA M, EDSOR E, et al . Assessment of quality of platelet-rich plasma produced with desktop centrifuge and comparison with standardized commercially available platelet-rich plasma[J ] . J Pharm Bioallied Sci, 2020, 12(Suppl 1): S607-S612. DOI: 10.4103/jpbs.JPBS_163_20.

中国输血协会临床输血管理学专业委员会. 自体富血小板血浆制备技术专家共识[J]. 中国输血杂志, 2021, 34(7): 677-683. DOI:10.13303/j.cjbt.issn.1004-549x.2021.07.001.

Professional Committee of Clinical Transfusion Management of China Transfusion Association. Expert consensus on preparation technology of autologous platelet rich plasma[J]. Chin J Blood Transfus, 2021, 34(7): 677-683. DOI: 10.13303/j.cjbt.issn.1004-549x.2021.07.001.

国家卫生健康委员会. 血站技术操作规程(2019版)[EB/OL]. [2019-12-31]. https://www.sbc.org.cn/upload/default/20190510/ef3ea3b66c019765da22a863be40f7ab.pdf.

National Health Commission. Technical operating procedures for blood stations(2019 version)[EB/OL]. [2019-12-31]. https://www.sbc.org.cn/upload/default/20190510/ef3ea3b66c019765da22a863be40f7ab.pdf.

单桂秋, 林放, 张爽, 等. 血小板拓展应用研究的认识与思考[J]. 中国输血杂志, 2016, 29(6): 555-557. DOI: 10.13303/j.cjbt.issn.1004-549x.2016.06.001.

SHAN G Q, LIN F, ZHANG S, et al . Understanding and thinking of platelet expansion and application research[J ] . Chin J Blood Transfus, 2016, 29(6): 555-557. DOI: 10.13303/j.cjbt.issn.1004-549x.2016.06.001.

GUPTA S, PALICZAK A, DELGADO D. Evidence-based indications of platelet-rich plasma therapy[J]. Expert Rev Hematol, 2021, 14(1): 97-108. DOI: 10.1080/17474086.2021.1860002.

FANG J, WANG X, JIANG W, et al . Platelet-rich plasma therapy in the treatment of diseases associated with orthopedic injuries[J ] . Tissue Eng Part B Rev, 2020, 26(6): 571-585. DOI: 10.1089/ten.TEB.2019.0292.

CAO Y Q, ZHU X X, ZHOU R X, et al . A na rrative review of the research progress and clinical application of platelet-rich plasma[J ] . Ann Palliat Med, 2021, 10(4): 4823-4829. DOI: 10.21037/apm-20-2223.

赵树铭, 史春梦, 李忠俊. 实用临床输血学[M]. 北京: 人民卫生出版社, 2015: 43.

ZHAO S M, SHI C M, LI Z J. Practical clinical blood transfusion[M]. Beijing: People's Health Publishing House, 2015: 43.

向仁雪, 吴建君, 王嘉嘉, 等. 手工与全自动法制备富血小板血浆的质量及治疗膝关节疾病的疗效分析[J]. 中国输血杂志, 2021, 34(7): 695-698. DOI: 10.13303/j.cjbt.issn.1004-549x.2021.07.006.

XIANG R X, WU J J, WANG J J, et al . Autologous platelet-rich plasma(PRP) for treatment of knee arthritis: a comparative study between PRP prepared by manual and automaticplatelet apheresis[J ] . Chin J Blood Transfus, 2021, 34(7): 695-698. DOI: 10.13303/j.cjbt.issn.1004-549x.2021.07.006.

李泰然, 陶凯, 陈胜, 等. 自体单采富血小板血浆修复慢性难愈合创面临床应用[J]. 创伤与急危重病医学, 2020, 8(5): 364-367. DOI: 10.16048/j.issn.2095-5561.2020.05.14.

LI T R, TAO K, CHEN S, et al . Clinical application of autologous single collection platelet-rich plasma in repairing chronic refractory wounds[J ] . Trauma Crit Care Med, 2020, 8(5): 364-367. DOI: 10.16048/j.issn.2095-5561.2020.05.14.

刘培焱, 周琦, 管俊, 等. 富血小板血浆制剂中白细胞在临床中的作用及其制备[J]. 中国输血杂志, 2020, 33(7): 725-729. DOI: 10.13303/j.cjbt.issn.1004-549x.2020.07.024.

LIU P Y, ZHOU Q, GUAN J, et al . Clinical effects of leukocytes within platelet-rich plasma preparations[J ] . Chin J Blood Transfus, 2020, 33(7): 725-729. DOI: 10.13303/j.cjbt.issn.1004-549x.2020.07.024.

易中梅, 蒋天伦, 徐霞. 1例术前自体血小板凝胶联合全血的预存应用[J]. 重庆医学, 2017, 46(2): 287-288. DOI: 10.3969/j.issn.1671-8348.2017.02.051.

YI Z M, JIANG T L, XU X. Preoperative application of auto logous platelet gel combined with whole blood in 1 cases[J]. Chongqing Med, 2017, 46(2): 287-288. DOI: 10.3969/j.issn.1671-8348.2017.02.051.

林惠凤. 实用血液净化护理[M]. 上海: 上海科学技术出版社, 2005.

LIN H F. Practical blood purification nursing[M]. Shanghai: Shanghai Science and Technology Press, 2005.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

长期摄入低剂量贫铀对大鼠血象与肝肾功能的影响