6-姜辣素改善果糖所致中年大鼠骨骼肌异位脂质沉积的研究

刘宇哲; 詹权操; 曾颜; 席雨蒙; 李冬; 王建伟

doi:10.16016/j.2097-0927.202110157

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6-姜辣素改善果糖所致中年大鼠骨骼肌异位脂质沉积的研究

基础医学 | 更新时间：2026-01-09

- 6-姜辣素改善果糖所致中年大鼠骨骼肌异位脂质沉积的研究
- 6-gingerol ameliorates ectopic lipid accumulation in skeletal muscle of middle-aged rats induced by fructose
- 陆军军医大学学报 2022年44卷第8期页码：765-773
- 作者机构：
  
  1.400016 重庆，重庆医科大学:基础医学院组织学与胚胎学教研室
  2.400016 重庆，中医药防治代谢性疾病重庆市重点实验室
  3.400016 重庆，重庆医科大学:中医药学院
- 作者简介：
  
  [王建伟，E-mail: wjwcq68@163.comWANG Jianwei, E-mail: wjwcq68@163.com
- 基金信息：
  
  国家自然科学基金面上项目(81973653)
- DOI：10.16016/j.2097-0927.202110157
  中图分类号： R285.5;R322.74;R589.2
- 收稿：2021-10-26，
  
  修回：2022-01-05，
  
  纸质出版：2022-04-30
- 稿件说明：
移动端阅览
刘宇哲, 詹权操, 曾颜, 等. 6-姜辣素改善果糖所致中年大鼠骨骼肌异位脂质沉积的研究[J]. 陆军军医大学学报, 2022,44(8):765-773.

Yuzhe LIU, Quancao ZHAN, Yan ZENG, et al. 6-gingerol ameliorates ectopic lipid accumulation in skeletal muscle of middle-aged rats induced by fructose[J]. Journal of Army Medical University, 2022, 44(8): 765-773.
刘宇哲, 詹权操, 曾颜, 等. 6-姜辣素改善果糖所致中年大鼠骨骼肌异位脂质沉积的研究[J]. 陆军军医大学学报, 2022,44(8):765-773. DOI： 10.16016/j.2097-0927.202110157.

Yuzhe LIU, Quancao ZHAN, Yan ZENG, et al. 6-gingerol ameliorates ectopic lipid accumulation in skeletal muscle of middle-aged rats induced by fructose[J]. Journal of Army Medical University, 2022, 44(8): 765-773. DOI： 10.16016/j.2097-0927.202110157.

摘要

目的

探究6-姜辣素改善果糖所致中年大鼠骨骼肌异位脂质沉积的作用及机制。

方法

动物实验:将24只15月龄雄性SD大鼠采用随机数字表法分为3组:对照组(CON，正常饮水)，模型组(FRU，10%果糖水)，6-姜辣素组(GIN，10%果糖水，0.2 mg/kg 6-姜辣素)，每组8只，6-姜辣素通过灌胃给药，每日1次，持续49 d。采用酶法检测血浆葡萄糖(GLU)、甘油三酯(TG)及骨骼肌组织TG水平；油红O染色(ORO)检测骨骼肌组织脂质沉积；ELISA检测血浆胰岛素(Insulin)；Western blot检测骨骼肌AMPK、p-AMPK、ACC、p-ACC、CPT1A、PPARα及PGC1α蛋白表达。细胞实验:将分化成熟的大鼠骨骼肌细胞分为3组:对照组(CON)，模型组(FRU，25 mmol/L果糖)，6-姜辣素组(GIN，25 mmol/L果糖，10 μmol/L 6-姜辣素)，作用24 h。酶法检测细胞内TG水平；ORO检测骨骼肌细胞脂质沉积；Western blot检测骨骼肌细胞AMPK、p-AMPK、ACC、p-ACC及CPT1A蛋白表达。

结果

与对照组比较，模型组血浆GLU、TG及Insulin水平明显升高(

0.05)，大鼠骨骼肌组织及骨骼肌细胞内TG含量明显升高(

0.05)，ORO示大鼠骨骼肌组织及骨骼肌细胞内脂质沉积明显，Western blot示p-AMPK/AMPK及p-ACC/ACC降低，CPT1A、PPARα及PGC1α蛋白表达明显降低(

0.05)；与模型组比较，姜辣素组血浆GLU、TG及Insulin水平明显降低(

0.05)，大鼠骨骼肌组织及骨骼肌细胞内TG含量显著降低(

0.05)，ORO示大鼠骨骼肌及骨骼肌细胞内脂质沉积减轻，Western blot示p-AMPK/AMPK及p-ACC/ACC明显升高，CPT1A、PPARα及PGC1α蛋白表达明显升高(

0.05)。

结论

6-姜辣素可改善果糖所致中年大鼠骨骼肌异位脂质沉积，可能与骨骼肌AMPK/ACC/CPT1A通路的激活有关。

Abstract

Objective

To investigate the effect and mechanism of 6-gingerol on improving ectopic lipid accumulation in skeletal muscle of middle-aged rats induced by fructose.

Methods

A total of 24 male SD rats (15 months old) were randomly and equally divided into 3 groups: control group (CON

with normal drinking water)

model group (FRU

with 10% fructose water) and 6-gingerol group (GIN

with 10% fructose water+0.2 mg/kg 6-gingerol by gavage). Gavage was performed once a day for consecutive 49 days. Levels of plasma glucose (GLU)

triglyceride (TG) and skeletal muscle TG were measured by GOD-PAP method; lipid accumulation in skeletal muscle was detected by oil red O staining (ORO); content of plasma insulin was determined by ELISA; protein levels of AMP-activated protein kinase (AMPK)

p-AMPK

acetyl-coa carboxylase (ACC)

p-ACC

carnitine palmitoyltransferase 1A (CPT1A)

peroxisome proliferator activated receptor alpha (PPARα) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α) in skeletal muscle were examined with Western blotting. L6 rat skeletal muscle cells were divided into 3 groups: control group (Con)

model group (FRU

treated with 25 mmol/L fructose for 24 h) and 6-gingerol group (GIN

treated with 25 mmol/L fructose+10 μmol/L 6-gingerol for 24 h). TG level and lipid accumulation in L6 cells were determined by GOD-PAP and ORO staining

respectively. The expression of AMPK

p-AMPK

ACC

p-ACC and CPT1A was examined by Western blotting.

Results

As compared with control group

the rat model group had higher levels of plasma GLU

TG and insulin (

0.05)

elevated TG content in both skeletal muscle tissues and cells (

0.05)

as well as notable lipid accumulation in skeletal muscle tissues and cells (

0.05). Western blotting showed that the p-AMPK/AMPK ratio

p-ACC/ACC ratio

and the protein levels of CPT1A

PPARα and PGC1α were all decreased in the model group (

0.05). However

6-gingerol significantly reduced the levels of plasma GLU

TG and insulin (

0.05)

alleviated the TG contents and lipid accumulation in skeletal muscles and cells (

0.05)

and greatly increased the p-AMPK/AMPK ratio

p-ACC/ACC ratio and the levels of CPT1A

PPARα and PGC1α in the GIN group (

0.05).

Conclusion

6-gingerol can improve the ectopic lipid accumulation in skeletal muscle of middle-aged rats induced by fructose

which may be related to the activation of AMPK/ACC/CPT1A pathway.

关键词

Keywords

references

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