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白藜芦醇调节NLRP3炎性小体改善力竭运动大鼠肾组织炎症损伤
Resveratrol improves renal inflammatory injury in exhaustive exercise rats by regulating NOD-like receptor protein 3 inflammasome
- 陆军军医大学学报 2022年44卷第12期 页码：1229-1236
- 作者机构：
1.453007 河南 新乡，河南师范大学体育学院
2.100084 北京，北京体育大学运动人体科学学院
- 作者简介：
[曹建民，E-mail：bsucaojianmin@aliyun.comCAO Jianmin, E-mail: bsucaojianmin@aliyun.com
- 基金信息：河南省重点研发与推广专项(科技攻关)项目(212102310261)
DOI：10.16016/j.2097-0927.202110114
中图分类号： R285.5;R692.3;R873.01收稿：2021-10-21，
修回：2022-01-16，
纸质出版：2022-06-30
- 稿件说明：
移动端阅览
李方, 曹建民, 翟鹏飞, 等. 白藜芦醇调节NLRP3炎性小体改善力竭运动大鼠肾组织炎症损伤[J]. 陆军军医大学学报, 2022,44(12):1229-1236.
Fang LI, Jianmin CAO, Pengfei ZHAI, et al. Resveratrol improves renal inflammatory injury in exhaustive exercise rats by regulating NOD-like receptor protein 3 inflammasome[J]. Journal of Army Medical University, 2022, 44(12): 1229-1236.
李方, 曹建民, 翟鹏飞, 等. 白藜芦醇调节NLRP3炎性小体改善力竭运动大鼠肾组织炎症损伤[J]. 陆军军医大学学报, 2022,44(12):1229-1236. DOI： 10.16016/j.2097-0927.202110114.
Fang LI, Jianmin CAO, Pengfei ZHAI, et al. Resveratrol improves renal inflammatory injury in exhaustive exercise rats by regulating NOD-like receptor protein 3 inflammasome[J]. Journal of Army Medical University, 2022, 44(12): 1229-1236. DOI： 10.16016/j.2097-0927.202110114.
摘要
目的
2
探讨白藜芦醇通过调节NOD样受体蛋白3(NOD-like receptor protein 3，NLRP3)炎性小体对力竭运动大鼠肾组织炎症损伤的改善作用。
方法
2
将36只SD雄性大鼠按随机数字表法分为对照组(Con组，
n
=8)、白藜芦醇组(Rsv组，
n
=8)、力竭运动组(Ex组，
n
=10)和力竭运动+白藜芦醇组(Ex+Rsv组，
n
=10)。Ex组和Ex+Rsv组进行4周力竭跑台运动。Rsv组和Ex+Rsv组每天灌胃白藜芦醇，剂量为50 mg/kg。最后1次运动24 h后取材，分别取血、尿和肾组织以备检测，全自动分析仪检测血清肌酐(serum creatinine，Scr)、尿素氮(blood urea nitrogen，BUN)水平；ELISA检测尿中性粒细胞明胶酶相关脂质运载蛋白(neutrophil gelatinaseassociated lipocalin，NGAL)和肾损伤分子-1 (kidney injury molecule-1，KIM-1)以及肾组织IL-1β、IL-18、TNF-α、IL-6水平；HE染色观察肾组织形态结构变化；Western blot检测肾组织NLRP3、凋亡相关斑点样蛋白(apoptosis-associated speck-like protein，ASC)和cleaved caspase-1、IL-1β和IL-18蛋白表达；免疫荧光双染观察NLRP3和ASC在肾小管的共定位。
结果
2
力竭运动组大鼠血清指标Scr及BUN、尿液指标KIM-1和NGAL水平均比对照组显著升高(
P
<
0.01)，肾组织HE染色显示存在肾组织损伤；力竭运动组大鼠肾组织NLRP3、ASC、cleaved caspase-1、IL-1β和IL-18蛋白表达均显著升高(
P
<
0.01)；白藜芦醇补充显著降低了力竭运动大鼠肾组织NLRP3炎性小体蛋白表达(
P
<
0.05)，改善其肾组织形态，降低肾组织炎症因子浓度(
P
<
0.05)。
结论
2
白藜芦醇可有效改善力竭运动大鼠肾组织炎症损伤，其机制可能与白藜芦醇抑制大鼠肾脏NLRP3炎性小体的过度激活有关。
Abstract
Objective
2
To explore the ameliorative effects of resveratrol on renal inflammation in rats after exhaustive exercise by regulating NOD-like receptor protein 3 (NLRP3) inflammasome.
Methods
2
Thirty-six male SD rats were randomly divided into 4 groups: control group (Con
n
=8)
resveratrol group (Rsv
n
=8)
exhaustive exercise group (Ex
n
=10)
and exhaustive exercise+resveratrol group (Ex+Rsv
n
=10)
and the rats in the Ex and Ex+Rsv groups underwent 4 weeks of exhaustive treadmill exercise. The Rsv and Ex+Rsv groups were given resveratrol intragastrically at a dose of 50 mg/kg per day. The serum and urine samples as well as renal tissues of rats were collected in 24 h after the last training. The serum creatinine (Scr) and blood urea nitrogen (BUN) levels were detected by automatic analyzer; ELISA was used to determine the urine neutrophil gelatinase-associated lipocalin (NGAL)
kidney injury molecule-1 (KIM-1) and the contents of IL-1β
IL-18
TNF-α and IL-6 in renal tissues; The protein expression of NLRP3
apoptosis-associated speck-like protein (ASC)
cleaved caspase-1
IL-1β and IL-18 were measured by Western blotting. In addition
the morphological and structural changes of renal tissue were observed with HE staining
and immunofluorescence staining was also performed to observe the co-localization of NLRP3 and ASC.
Results
2
The levels of Scr
BUN
NGAL and KIM-1 were significantly higer in the Ex group than the Con group (
P
<
0.01). HE staining showed that renal injury was observed in the rats of Ex group
and the protein expression levels of NLRP3
ASC
cleaved caspase-1
IL-1β and IL-18 were all remarkably increased (
P
<
0.01). However
resveratrol supplementation notably down-regulated the protein e
xpression of NLRP3 inflammasome (
P
<
0.05) and reduced the contents of inflammatory factors in renal tissues (
P
<
0.05)
and ameliorated pathological changes of renal tissues.
Conclusions
2
Resveratrol can effectively alleviate the inflammatory damage of renal tissue in rats after exhaustive exercise
and its mechanism may be related to its inhibiting NLRP3 inflammasome overactivation.
关键词
Keywords
references
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