海马PGC-1α对小鼠抑郁样行为及海马各亚区星形胶质细胞数量的影响

邓宇辉; 罗艳敏; 梁芯; 蒋林; 王瑾; 唐静; 李静; 黎悦; 肖凯; 祝佩林; 郭一静; 唐勇; 吴宏

doi:10.16016/j.2097-0927.202110068

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海马PGC-1α对小鼠抑郁样行为及海马各亚区星形胶质细胞数量的影响

神经科学 | 更新时间：2026-01-09

- 海马PGC-1α对小鼠抑郁样行为及海马各亚区星形胶质细胞数量的影响
- Effects of hippocampal PGC-1α on depression-like behaviors and number of astrocytes in hippocampal subregions in mice
- 陆军军医大学学报 2022年44卷第10期页码：975-983
- 作者机构：
  
  1.400016 重庆，重庆医科大学基础医学院组织与胚胎学教研室
  2.400016 重庆，重庆医科大学基础医学院干细胞与组织工程研究室
  3.400016 重庆，重庆医科大学基础医学院生理学教研室
  4.400016 重庆，重庆医科大学基础医学院病理生理学教研室
  5.400016 重庆，重庆医科大学基础医学院实验教学管理中心
- 作者简介：
  
  [吴宏，E-mail：wuhong6368@126.com WU Hong, E-mail: wuhong6368@126.com
- 基金信息：
  
  国家自然科学基金面上项目(81871073);国家自然科学基金青年科学基金(82001436);重庆市教育委员会科学技术研究计划(KJQN202000402)
- DOI：10.16016/j.2097-0927.202110068
  中图分类号： R319;R322.81;R749.4
- 收稿：2021-10-13，
  
  修回：2021-12-17，
  
  纸质出版：2022-05-30
- 稿件说明：
移动端阅览
邓宇辉, 罗艳敏, 梁芯, 等. 海马PGC-1α对小鼠抑郁样行为及海马各亚区星形胶质细胞数量的影响[J]. 陆军军医大学学报, 2022,44(10):975-983.

Yuhui DENG, Yanmin LUO, Xin LIANG, et al. Effects of hippocampal PGC-1α on depression-like behaviors and number of astrocytes in hippocampal subregions in mice[J]. Journal of Army Medical University, 2022, 44(10): 975-983.
邓宇辉, 罗艳敏, 梁芯, 等. 海马PGC-1α对小鼠抑郁样行为及海马各亚区星形胶质细胞数量的影响[J]. 陆军军医大学学报, 2022,44(10):975-983. DOI： 10.16016/j.2097-0927.202110068.

Yuhui DENG, Yanmin LUO, Xin LIANG, et al. Effects of hippocampal PGC-1α on depression-like behaviors and number of astrocytes in hippocampal subregions in mice[J]. Journal of Army Medical University, 2022, 44(10): 975-983. DOI： 10.16016/j.2097-0927.202110068.

摘要

目的

探讨定向沉默/过表达小鼠海马中过氧化物酶体增殖物激活受体γ辅激活因子1α(PPAR gamma coactivator 1 alpha，PGC-1α)对小鼠抑郁样行为和海马各亚区胶质纤维酸性蛋白(glial fibrillary acidic protein，GFAP)阳性(

)星形胶质细胞数量的影响。

方法

选取32只6~8周龄的雄性C57BL/6小鼠，在经过适应性喂养和糖水偏好基线调整后按照简单随机抽样方法分为空病毒组(

=14)、PGC-1α沉默组(

=11)和PGC-1α过表达组(

=7)，分别采用海马立体定位注射技术以腺相关病毒为载体定向沉默/过表达小鼠海马中PGC-1α基因。运用糖水偏好实验和强迫游泳实验评估小鼠的抑郁样行为，采用免疫组织化学和现代体视学相结合的方法对小鼠海马各亚区中GFAP

星形胶质细胞数量进行精确三维定量研究。

结果

与空病毒组小鼠相比，PGC-1α沉默组小鼠的糖水偏好百分比显著降低(

0.01)，强迫游泳实验不动时间显著增加(

0.05)，海马CA1和DG区中的GFAP

星形胶质细胞数量显著增加(

0.05)；而PGC-1α过表达组小鼠的强迫游泳实验不动时间显著减少(

0.01)，海马CA1和DG区中的GFAP

星形胶质细胞数量显著减少(

0.05)。空病毒组与PGC-1α过表达组小鼠的糖水偏好百分比差异无统计学意义。沉默/过表达海马PGC-1α对CA3区星形胶质细胞数量的影响差异无统计学意义。

结论

小鼠海马PGC-1α与抑郁样行为密切相关，并参与海马CA1和DG区内GFAP

星形胶质细胞数量的调节。

Abstract

Objective

To investigate the effects of targeted silence/overexpression of PGC-1α in the hippocampus on depression-like behaviors in mice and on the number of glial fibrillary acidic protein positive (GFAP

) astrocytes in the subregions of the hippocampus.

Methods

After adaptive feeding and sucrose preference baseline adjustment

32 male C57BL/6 mice aged 6~8 weeks were randomly divided into empty virus group (

=14)

PGC-1α silencing group (

=11) and PGC-1α overexpression group (

=7). The latter 2 groups were injected with adeno-associated virus (AAV)-mediated genes silencing or overexpressing PGC-1α in the hippocampus

respectively

with aid of stereotactic injection technique. Sucrose preference test (SPT) and forced swimming test (FST) were performed to assess the depression-like behaviors

and the number of GFAP

astrocytes in each hippocampal subregion was accurately quantified in 3 dimensions by immunohistochemistry and stereology.

Results

As compared with the empty virus group

the PGC-1α silencing group had significantly decreased percentage of sucrose preference (

0.01)

prolonged immobility time in FST (

0.05)

and larger number of GFAP

astrocytes in both CA1 and DG subregions(

0.05). However

the immobi

lity time in FST was remarkably shortened in the PGC-1α overexpression group (

0.01)

with the number of GFAP

astrocytes in CA1 and DG subregions diminished (

0.05). There was no significant difference in the percentage of sucrose preference between the empty virus group and PGC-1α overexpression group

and silencing or overexpressing PGC-1α showed no obvious impact on the number of astrocytes in CA3 subregion.

Conclusion

PGC-1α is closely associated with the depression-like behaviors and participates in regulating the number of GFAP

astrocytes in CA1 and DG subregions in mice.

关键词

Keywords

references

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