高盐饮食抑制小鼠NK细胞成熟和杀伤功能的研究

陶洪云; 童师雯; 殷文伟

doi:10.16016/j.2097-0927.202110039

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高盐饮食抑制小鼠NK细胞成熟和杀伤功能的研究

基础医学 | 更新时间：2026-01-09

- 高盐饮食抑制小鼠NK细胞成熟和杀伤功能的研究
- High-salt diet inhibits maturation and killing function of NK cells in mice
- 陆军军医大学学报 2022年44卷第9期页码：882-890
- 作者机构：
  
  400010 重庆，重庆医科大学附属第二医院感染科，病毒性肝炎研究所
- 作者简介：
  
  [殷文伟，E-mail：yww@cqmu.edu.cn YIN Wenwei, E-mail: yww@cqmu.edu.cn
- 基金信息：
  
  国家自然科学基金面上项目(81770570);重庆市渝中区基础研究与前沿探索项目(030)
- DOI：10.16016/j.2097-0927.202110039
  中图分类号： R151.1;R392.12
- 收稿：2021-10-10，
  
  修回：2021-12-07，
  
  纸质出版：2022-05-15
- 稿件说明：
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陶洪云, 童师雯, 殷文伟. 高盐饮食抑制小鼠NK细胞成熟和杀伤功能的研究[J]. 陆军军医大学学报, 2022,44(9):882-890.

Hongyun TAO, Shiwen TONG, Wenwei YIN. High-salt diet inhibits maturation and killing function of NK cells in mice[J]. Journal of Army Medical University, 2022, 44(9): 882-890.
陶洪云, 童师雯, 殷文伟. 高盐饮食抑制小鼠NK细胞成熟和杀伤功能的研究[J]. 陆军军医大学学报, 2022,44(9):882-890. DOI： 10.16016/j.2097-0927.202110039.

Hongyun TAO, Shiwen TONG, Wenwei YIN. High-salt diet inhibits maturation and killing function of NK cells in mice[J]. Journal of Army Medical University, 2022, 44(9): 882-890. DOI： 10.16016/j.2097-0927.202110039.

摘要

目的

探究高盐饮食对小鼠自然杀伤(natural killer，NK)细胞成熟和功能的影响。

方法

3~4周龄雄性C57BL/6J小鼠(体质量约9 g)分为正常盐饮食(normal-salt diet，NSD)组(给予普通饲料及高压灭菌水喂养)和高盐饮食(high-salt diet，HSD)组(给予含4% NaCl高盐饲料及含1% NaCl高压灭菌水喂养)。采用CD27和CD11b的表达界定NK细胞成熟亚群，CD69标记细胞的早期活化，流式细胞术分析两组小鼠NK细胞亚群分布和活化水平；采用polyI: C体内处理18 h和体外佛波酯/离子霉素刺激4 h，流式细胞术检测两组小鼠NK细胞IFN-γ产生水平；通过YAC-1体内杀伤实验，流式细胞术评估两组小鼠NK细胞的细胞毒作用。

结果

高盐饮食不影响肝脏﹑脾脏﹑骨髓和外周血NK细胞比例，但抑制肝脏、脾脏NK细胞CD69表达(

0.01)。高盐饮食组小鼠较正常盐饮食组小鼠肝、脾、骨髓中未成熟的NK细胞(CD27

CD11b

)比例增加(

0.05)，而成熟的M1 NK细胞亚群(CD27

CD11b

)比例降低(

0.05)，DX5

NK1.1

成熟NK细胞比例也降低(

0.05)，表明高盐饮食抑制NK细胞成熟。高盐饮食组小鼠NK细胞IFN-γ的表达低于正常盐饮食组小鼠(

0.01)，提示高盐饮食抑制NK细胞功能。体内杀伤实验表明，高盐饮食组小鼠腹腔剩余YAC-1细胞百分比和绝对数升高(

0.01)，提示高盐饮食抑制NK细胞的细胞毒作用。

结论

高盐饮食抑制NK细胞成熟，抑制NK细胞的活化和功能。

Abstract

Objective

To investigate the effects of high-salt diet on the maturation and function of NK cells.

Methods

Mouse models of normal salt diet (NSD) and high salt diet (HSD) were established by feeding food and drinking water with different salt content. The expression of CD27 and CD11b was used to define the NK cell maturation subsets

and the early activation of cells was labeled by CD69. Flow cytometry was employed to analyze the distribution of NK cell subsets and activation in NSD and HSD mice. The IFN-γ production level of NK cells from mice treated with polyI: C for 18 h

in vivo

and stimulated with PMA/Ionomycin for 4 h

in vitro

was detected by flow cytometry. Cytotoxic activity of NK cells was determined through the YAC-1 killing experiment

in vivo

Results

HSD showed no effect on the percentage of NK cells in the liver

spleen

bone marrow and peripheral blood

but inhibited the expression of CD69 in liver and spleen NK cells (

0.01). The percentage of immature NK cells (CD27

CD11b

) subsets was significantly higher in the liver

spleen and bone marrow from HSD mice when compared to those in NSD mice (

0.05)

while the percentage of mature M1 NK cell subsets (CD27

CD11b

) as well as DX5

NK1.1

mature NK cells were markedly lower in HSD mice (

0.05)

suggesting that HSD inhibits the maturation of NK cells. The expression of IFN-γ in NK cells of HSD mice was lower than that of NSD mice (

0.01)

indicating that HSD inhibited NK cell function. Moreover

YAC-1

in vivo

killing experiment showed that the percentage and absolute number of residual YAC-1 cells in the abdominal cavity of HSD mice were higher than those of NSD mice (

0.01)

indicating that HSD inhibited the cytotoxicity of NK cells.

Conclusion

HSD inhibits the maturation of NK cells as well as the activation and function of NK cells.

关键词

Keywords

references

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