50 Hz磁场和双酚A暴露对大鼠脑组织和Neuro-2a细胞Tau蛋白磷酸化的影响

张彦文; 吕艳玲; 马秦龙; 高鹏; 陈纯海; 何旻蒂; 张蕾; 卢永辉; 皮会丰; 余争平

doi:10.16016/j.2097-0927.202110024

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50 Hz磁场和双酚A暴露对大鼠脑组织和Neuro-2a细胞Tau蛋白磷酸化的影响

公共卫生与预防医学 | 更新时间：2026-01-09

- 50 Hz磁场和双酚A暴露对大鼠脑组织和Neuro-2a细胞Tau蛋白磷酸化的影响
- Effects of 50-Hz magnetic field and bisphenol A exposure on phosphorylation of Tau protein in rat brain tissue and Neuro-2a cells
- 陆军军医大学学报 2022年44卷第9期页码：950-959
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)军事预防医学系军队劳动卫生学教研室，电磁辐射医学防护教育部重点实验室
- 作者简介：
  
  [张彦文，E-mail: zhangyw20022002@aliyun.comZHANG Yanwen, E-mail: zhangyw20022002@aliyun.com
- 基金信息：
  
  国家自然科学基金面上项目(31770906)
- DOI：10.16016/j.2097-0927.202110024
  中图分类号： R122.4;R136.32;R322.81
- 收稿：2021-10-08，
  
  修回：2021-12-06，
  
  纸质出版：2022-05-15
- 稿件说明：
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张彦文, 吕艳玲, 马秦龙, 等. 50 Hz磁场和双酚A暴露对大鼠脑组织和Neuro-2a细胞Tau蛋白磷酸化的影响[J]. 陆军军医大学学报, 2022,44(9):950-959.

Yanwen ZHANG, Yanling LYU, Qinlong MA, et al. Effects of 50-Hz magnetic field and bisphenol A exposure on phosphorylation of Tau protein in rat brain tissue and Neuro-2a cells[J]. Journal of Army Medical University, 2022, 44(9): 950-959.
张彦文, 吕艳玲, 马秦龙, 等. 50 Hz磁场和双酚A暴露对大鼠脑组织和Neuro-2a细胞Tau蛋白磷酸化的影响[J]. 陆军军医大学学报, 2022,44(9):950-959. DOI： 10.16016/j.2097-0927.202110024.

Yanwen ZHANG, Yanling LYU, Qinlong MA, et al. Effects of 50-Hz magnetic field and bisphenol A exposure on phosphorylation of Tau protein in rat brain tissue and Neuro-2a cells[J]. Journal of Army Medical University, 2022, 44(9): 950-959. DOI： 10.16016/j.2097-0927.202110024.

摘要

目的

研究50 Hz磁场、双酚A(bisphenol A，BPA)单独和联合暴露对神经细胞Tau蛋白磷酸化的影响。

方法

24只SD雄性大鼠，体质量(150±10) g，分为对照组(生理盐水灌胃+50 Hz磁场假性暴露)、50 Hz磁场暴露组(0.5 mT、4 h/d 50 Hz磁场暴露+生理盐水灌胃)、BPA暴露组(BPA 20 μg·kg

-1

·d

-1

灌胃+50 Hz磁场假性暴露)、50 Hz磁场和BPA复合暴露组(BPA 20 μg·kg

-1

·d

-1

灌胃+0.5 mT、4 h/d 50 Hz磁场暴露)4组，每组6只。处理1次/d，6 d/周，连续12周。小鼠神经母细胞瘤细胞株(Neuro-2a)，分为对照组、50 Hz磁场暴露组(1 mT、48 h)、BPA暴露组(100 μmol/L、24 h)、50 Hz磁场+BPA复合暴露组(1 mT、48 h +100 μmol/L

24 h)4组。大鼠暴露结束按标准方法取材，组织免疫荧光化学方法检测大脑皮层和海马脑组织Tau-Thr

231

、Tau-Ser

404

的表达以及细胞凋亡。细胞实验暴露后采用CCK-8法检测细胞活力，采用细胞免疫荧光化学和Western blot方法检测Tau-Thr

231

、Tau-Ser

404

、Caspase-3的表达。

结果

50 Hz磁场暴露12周后，大鼠皮层和海马脑区Tau-Thr

231

位点磷酸化水平明显升高(

0.05)；BPA暴露或与50 Hz磁场复合暴露12周后，大鼠皮层和海马脑区Tau-Thr

231

位点磷酸化水平、皮层Tau-Ser

404

位点磷酸化水平明显升高(

0.05)，复合暴露组皮层和海马脑区细胞凋亡水平变化明显(

0.05)。50 Hz磁场暴露、BPA单独暴露或与50 Hz磁场复合暴露48 h后，Neuro-2a细胞活力均明显低于对照组(

0.05)。50 Hz磁场暴露48 h后，Neuro-2a细胞Tau-Ser

404

位点磷酸化水平明显高于对照组(

0.05)；BPA单独暴露或与50 Hz磁场复合暴露48 h后，Neuro-2a细胞Tau-Thr

231

位点和Tau-Ser

404

位点磷酸化水平、Caspase-3的表达水平明显升高(

0.05)。

结论

50 Hz磁场、BPA暴露可引起神经细胞Tau蛋白不同位点过度磷酸化，并且二者之间存在协同效应，可引起神经细胞损伤。

Abstract

Objective

To investigate the effects of 50-Hz magnetic field and bisphenol A (BPA) alone or combined exposure on the phosphorylation of Tau protein in nerve cells.

Methods

A total of 24 SD male rats (body weight 150±10 g) were randomly and equally divided into 4 groups: control group (normal saline gavage+magnetic field sham-exposure)

50-Hz magnetic field exposure group (0.5 mT

50 Hz magnetic field exposure 4 h/d+normal saline gavage)

BPA exposure group (BPA 20 μg·kg

-1

·d

-1

gavage+ magnetic field sham-exposure)

and combined exposure group (BPA 20 μg·kg

-1

·d

-1

gavage+0.5 mT

50 Hz magnetic field exposure 4 h/d). The magnetic field exposure was performed once per day

6 days per week

for consecutive 12 weeks. The protein expression of Tau-Thr

231

and Tau-Ser

404

as well as cell apoptosis in frozen slices of cerebral cortex and hippocampus were detected by immunohistofluorescence assay and TUNEL assay

respectively. For cell experiments

Neuro-2a cells were exposed to 50 Hz magnetic field (1.0 mT

48 h)

BPA treatment (100 μmol/L

24 h)

as well as combined exposure (50 Hz magnetic field+BPA)

respectively. Cell viability was tested by CCK-8 assay

and the protein expression of Tau-Thr

231

Tau-Ser

404

and Caspase-3 were measured using immunofluorescence assay and Western blotting.

Results

As compared with the control group

the phosphorylation of Tau-Thr

231

was significantly increased in cerebral cortex

and hippocampus of rats after 12 weeks of 50 Hz magnetic field exposure (

0.05). The Tau-Thr

231

phosphorylation in rat cerebral cortex and hippocampus and the Tau-Ser

404

phosphorylation in cerebral cortex were remarkably elevated after 12 weeks of BPA exposure alone or in combination with 50 Hz magnetic field (

0.05). The co-exposure also induced obvious neuronal apoptosis in cerebral cortex and hippocampus of rats (

0.05). For cell experiments

the Neuro-2a cell viability was greatly lowered after exposure to 50 Hz magnetic field

BPA alone or combined treatment for 48 h (

0.05). The Tau-Ser

404

phosphorylation was notably up-regulated (

0.05) after 50 Hz magnetic field exposure; the phosphorylation of Tau-Thr

231

and Tau-Ser

404

as well as the expression of Caspase-3 were also enhanced (

0.05) after BPA exposure or co-exposure.

Conclusion

Exposure to 50 Hz magnetic field and/or BPA can induce hyperphosphorylation of Tau protein at different amine acid site

and there are synergistic effects between the 2 exposures

which may cause nerve cell damage.

关键词

Keywords

references

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