环状RNA circACTN4促进乳腺癌细胞增殖、侵袭和转移的研究

欧小强; 陈俊霞

doi:10.16016/j.2097-0927.202109206

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环状RNA circACTN4促进乳腺癌细胞增殖、侵袭和转移的研究

基础医学 | 更新时间：2026-01-09

- 环状RNA circACTN4促进乳腺癌细胞增殖、侵袭和转移的研究
- Circular RNA circACTN4 promotes proliferation, invasion and metastasis of breast cancer cells
- 陆军军医大学学报 2022年44卷第7期页码：639-647
- 作者机构：
  
  400016 重庆，重庆医科大学基础医学院细胞生物学与遗传学教研室
- 作者简介：
  
  [陈俊霞, E-mail: chjunxia@126.comCHEN Junxia, E-mail: chjunxia@126.com
- 基金信息：
  
  国家自然科学基金面上项目(82173170);国家自然科学基金面上项目(82103089)
- DOI：10.16016/j.2097-0927.202109206
  中图分类号： R394.3;R730.23;R737.9
- 收稿：2021-09-28，
  
  修回：2021-11-07，
  
  纸质出版：2022-04-15
- 稿件说明：
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欧小强, 陈俊霞. 环状RNA circACTN4促进乳腺癌细胞增殖、侵袭和转移的研究[J]. 陆军军医大学学报, 2022,44(7):639-647.

Xiaoqiang OU, Junxia CHEN. Circular RNA circACTN4 promotes proliferation, invasion and metastasis of breast cancer cells[J]. Journal of Army Medical University, 2022, 44(7): 639-647.
欧小强, 陈俊霞. 环状RNA circACTN4促进乳腺癌细胞增殖、侵袭和转移的研究[J]. 陆军军医大学学报, 2022,44(7):639-647. DOI： 10.16016/j.2097-0927.202109206.

Xiaoqiang OU, Junxia CHEN. Circular RNA circACTN4 promotes proliferation, invasion and metastasis of breast cancer cells[J]. Journal of Army Medical University, 2022, 44(7): 639-647. DOI： 10.16016/j.2097-0927.202109206.

摘要

目的

探讨环状RNA circACTN4(hsa_circ_0050900)在乳腺癌中的表达及其对乳腺癌细胞增殖、侵袭和转移的影响。

方法

利用环状RNA芯片分析4对乳腺癌组织和癌旁组织的circRNA表达谱，并用实时荧光定量PCR验证circACTN4的相对表达水平；用circACTN4过表达质粒及对照质粒分别转染乳腺癌细胞MCF-7/MDA-MB-231，采用CCK-8实验、EdU实验、平板克隆实验、细胞划痕实验、Transwell迁移和侵袭实验研究circACTN4在乳腺癌细胞增殖、迁移和侵袭中的作用；Western blot检测circACTN4对CCND1、CCND2和CDK4蛋白表达的影响；在裸鼠体内探讨circACTN4对移植瘤生长转移的影响。

结果

CircACTN4在乳腺癌组织中的表达高于癌旁组织(

0.01)，在乳腺癌细胞中的表达高于正常乳腺上皮细胞(

0.01)。上调circACTN4可增强乳腺癌细胞增殖、迁移和侵袭(

0.05)，提高CCND1、CCND2和CDK4蛋白的表达(

0.01)。体内实验结果表明：上调circACTN4可促进移植瘤生长与转移，与对照组相比，circACTN4组肿瘤微血管密度显著增加(

0.05)，肺转移结节数量明显增多(

0.05)。

结论

circACTN4在乳腺癌组织和细胞显著高表达，可增强乳腺癌细胞的增殖、迁移和侵袭，并在体内促进移植瘤的生长与转移。

Abstract

Objective

To investigate the expression of circular RNA circACTN4 (also called hsa_circ_0050900) in breast cancer (BC) and its effect on the proliferation

invasion and metastasis of BC cells.

Methods

The circRNA expression profiles of 4 pairs of BC tissues and adjacent tissues were analyzed by circRNA chip analysis

and the relative expression level of circACTN4 was detected by qRT-PCR. After BC cell lines MCF-7/MDA-MB-231 were transfected with circACTN4 overexpression plasmid and control plasmid

respectively

the effects of circACTN4 on cell proliferation

migration and invasion were tested by Cell Counting Kit-8 (CCK-8)

EdU assay

colony formation

scratching assay

and Transwell migration and invasion assay. Western blotting was executed to determine the protein expression of CCND1

CCND2 and CDK4. The effects of circACTN4 on the growth and metastasis of transplanted tumors were also observed in nude mice.

Results

The expression of circACTN4 was significantly higher in BC tissues and cells than in the paired adjacent tissues and normal breast epithelial cells (

0.01). Up-regulation of circACTN4 enhanced the proliferation

migration and invasion of BC cells (

0.05)

and facilitated the expression of CCND1

CCND2 and CDK4 (

0.01). The results of

in vivo

experiments also showed that up-regulation of circACTN4 promoted the growth and metastasis of transplanted tumors

with the tumor microvascular density and the number of lung metastatic nodules significantly increased in the circACTN4 group

as compared with the control group (

0.05).

Conclusion

circACTN4 is highly expressed in BC tissues and cells. It can promote the proliferation

migration and invasion of BC cells

and facilitate the growth and metastasis of transplanted tumors

in vivo

关键词

Keywords

references

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