黄芩苷通过NF-κB信号对气道上皮黏液调节因子MUC5AC和CFTR表达的影响

张艳; 张彩丽; 刘燕; 陈玉龙; 彭梦薇; 桑亚洲; 吴耀松

doi:10.16016/j.2097-0927.202109016

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黄芩苷通过NF-κB信号对气道上皮黏液调节因子MUC5AC和CFTR表达的影响

基础医学 | 更新时间：2026-01-09

- 黄芩苷通过NF-κB信号对气道上皮黏液调节因子MUC5AC和CFTR表达的影响
- Effects of baicalin on expression of mucin 5AC and cystic fibrosis transmembrane conductance regulator in airway epithelium through NF-κB signaling pathway
- 陆军军医大学学报 2022年44卷第5期页码：441-447
- 作者机构：
  
  1.450046 郑州, 河南中医药大学中医药科学院，河南省中医方证信号传导重点实验室/河南省中医方证信号传导国际联合重点实验室
  2.450046 郑州, 河南中医药大学中医药科学院，河南省中西医结合防治脑认知疾病工程技术研究中心
  3.450046 郑州, 河南中医药大学中医药科学院，郑州市中西医结合防治脑认知疾病重点实验室
- 作者简介：
  
  [ 吴耀松, E-mail: linawys@163.com WU Yaosong, E-mail: linawys@163.com
- 基金信息：
  
  国家自然科学基金青年科学基金(81503490);河南省科技攻关计划(192102310160)
- DOI：10.16016/j.2097-0927.202109016
  中图分类号： R285.5;R322.34;R394.6
- 收稿：2021-09-02，
  
  修回：2021-11-03，
  
  纸质出版：2022-03-15
- 稿件说明：
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张艳, 张彩丽, 刘燕, 等. 黄芩苷通过NF-κB信号对气道上皮黏液调节因子MUC5AC和CFTR表达的影响[J]. 陆军军医大学学报, 2022,44(5):441-447.

Yan ZHANG, Caili ZHANG, Yan LIU, et al. Effects of baicalin on expression of mucin 5AC and cystic fibrosis transmembrane conductance regulator in airway epithelium through NF-κB signaling pathway[J]. Journal of Army Medical University, 2022, 44(5): 441-447.
张艳, 张彩丽, 刘燕, 等. 黄芩苷通过NF-κB信号对气道上皮黏液调节因子MUC5AC和CFTR表达的影响[J]. 陆军军医大学学报, 2022,44(5):441-447. DOI： 10.16016/j.2097-0927.202109016.

Yan ZHANG, Caili ZHANG, Yan LIU, et al. Effects of baicalin on expression of mucin 5AC and cystic fibrosis transmembrane conductance regulator in airway epithelium through NF-κB signaling pathway[J]. Journal of Army Medical University, 2022, 44(5): 441-447. DOI： 10.16016/j.2097-0927.202109016.

摘要

目的

基于核因子κB(nuclear factor kappa B，NF-κB)信号探讨黄芩苷调控气道上皮黏蛋白5AC(mucin 5AC

MUC5AC)和囊性纤维化跨膜传导调节因子(cystic fibrosis transmembrane conductance regulator，CFTR)的表达以改善病理性气道黏液的机制。

方法

采用MTT法检测不同浓度黄芩苷对H292细胞(人肺癌细胞)活性的影响并确定用于后续实验的黄芩苷浓度；将细胞分为空白对照组、模型组、NF-κB抑制剂组(PDTC组，100 μmol/L)和黄芩苷组(200 μg/mL)。采用IL-1β诱导H292细胞建立体外黏液高分泌炎症细胞模型，再以相应浓度的药物干预24 h后，采用ELISA方法检测各组细胞上清中MUC5AC、IL-8、TNF-α的含量，检测各组细胞中MUC5AC和CFTR蛋白的含量；采用实时荧光定量PCR检测各组细胞中MUC5AC和CFTR mRNA的表达；采用Western blot检测各组细胞中p65和IκB的表达。

结果

MTT检测结果显示，当黄芩苷浓度为200 μg/mL时，细胞活性在80%以上，可作为后续实验的安全浓度。与空白对照组比较，模型组细胞培养上清及细胞质中MUC5AC分泌水平及IL-8、TNF-α、p65、MUC5AC mRNA表达均明显升高(

0.05)，IκB蛋白表达、CFTR蛋白含量、CFTR mRNA的表达显著降低(

0.05)；与模型组比较，PDTC组细胞培养上清中MUC5AC分泌水平，IL-8、TNF-α、p65蛋白表达及MUC5AC mRNA表达显著降低(

0.05)，细胞质中MUC5AC水平、CFTR蛋白含量、IκB蛋白表达、CFTR mRNA的表达显著升高(

0.05)；与模型组比较，黄芩苷组细胞培养上清中MUC5AC分泌水平、IL-8和TNF-α分泌水平、p65蛋白表达降低(

0.01)，CFTR蛋白含量、IκB蛋白表达、CFTR mRNA表达均显著增加(

0.01)，而MUC5AC mRNA表达差异无统计学意义。

结论

黄芩苷通过抑制NF-κB活性，减少MUC5AC的分泌，增加CFTR的表达，减轻气道炎症。

Abstract

Objective

To investigate the mechanism of baicalin in improving pathological airway mucus by regulating the expression of airway epithelial mucus regulators mucin 5AC (MUC5AC) and cystic fibrosis transmembrane conductance regulator (CFTR) through nuclear factor κB (NF-κB) signaling pathway.

Methods

MTT assay was used to detect the effects of different concentrations of baicalin on the viability of human lung cancer H292 cells in order to determine the proper dose of baicalin for subsequent experiments. After H292 cells were induced by IL-1β to establish

in vitro

inflammatory cell model of mucus hypersecretion

the cells were divided into blank control group

model group

NF-κB inhibitor group (PDTC group

100 μmol/L) and baicalin group (200 μg/mL). After 24 h of drug intervention

the contents of MUC5AC

IL-8

and TNF-α in the culture supernatant and the levels of MUC5AC and CFTR in the cytoplasm of each group were detected by ELISA. Real-time fluorescent quantitative PCR was adopted to detect the mRNA expression of MUC5AC and CFTR. Western blotting was performed to test the expression of p65 and IκB (inhibitor of NF-κB).

Results

The results of MTT assay showed that 200 μg/mL was a safe concentration of baicalin for subsequent experiments

with cell viability larger than 80%. As compared with the blank control group

the protein expression of MUC5AC

IL-8

TNF-α

p65 and the mRNA level of MUC5AC in culture supernatant and cytoplasm were all significantly increased in the model group (

0.05)

while the expression of IκB and CFTR proteins as well as the CFTR mRNA level were remarkably decreased (

0.05). When compared with the model group

the PDTC group presented lower level of MUC5AC secretion

declined protein levels of IL-8

TNF-α and p65

as well as MUC5AC mRNA in the culture supernatant (

0.05)

while higher protein expression of MUC5AC

CFTR and IκB in the cytoplasm

along with elevated mR

NA level of CFTR (

0.05). In terms of the baicalin group

the secretion levels of MUC5AC

IL-8 and TNF-α were greatly diminished in culture supernatant (

0.01) as compared with the model group

whereas the expression of IκB as well as the protein and mRNA levels of CFTR notably improved (

0.01)

and there was no significant difference in MUC5AC mRNA expression.

Conclusion

Baicalin reduces MUC5AC secretion and increases CFTR expression by inhibiting the activity of NF-κB

and thus attenuates airway inflammation.

关键词

Keywords

references

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