幽门螺杆菌感染对胃黏膜上皮细胞REDD1表达的影响及调控机制

闫宗宝; 毛方圆; 单治国; 赵永亮; 庄园

doi:10.16016/j.2097-0927.202107181

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幽门螺杆菌感染对胃黏膜上皮细胞REDD1表达的影响及调控机制

基础医学 | 更新时间：2026-01-09

- 幽门螺杆菌感染对胃黏膜上皮细胞REDD1表达的影响及调控机制
- Effect and mechanism of H. pylori in regulation of REDD1 expression in gastric mucosa
- 陆军军医大学学报 2022年44卷第5期页码：413-420
- 作者机构：
  
  1.400038 重庆，陆军军医大学(第三军医大学)第一附属医院普通外科
  2.400038 重庆，陆军军医大学(第三军医大学)药学与检验医学系微生物与生化药学教研室
- 作者简介：
  
  [ 赵永亮，E-mail: yongliang1666@126.com ZHAO Yongliang, E-mail: yongliang1666@126.com
  [ 庄园，E-mail: yuanzhuang1983@yahoo.com ZHUANG Yuan, E-mail: yuanzhuang1983@yahoo.com
- 基金信息：
  
  国家自然科学基金面上项目(81872016)
- DOI：10.16016/j.2097-0927.202107181
  中图分类号： R322.44;R377;R394.3
- 收稿：2021-07-29，
  
  修回：2021-11-11，
  
  纸质出版：2022-03-15
- 稿件说明：
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闫宗宝, 毛方圆, 单治国, 等. 幽门螺杆菌感染对胃黏膜上皮细胞REDD1表达的影响及调控机制[J]. 陆军军医大学学报, 2022,44(5):413-420.

Zongbao YAN, Fangyuan MAO, Zhiguo SHAN, et al. Effect and mechanism of H. pylori in regulation of REDD1 expression in gastric mucosa[J]. Journal of Army Medical University, 2022, 44(5): 413-420.
闫宗宝, 毛方圆, 单治国, 等. 幽门螺杆菌感染对胃黏膜上皮细胞REDD1表达的影响及调控机制[J]. 陆军军医大学学报, 2022,44(5):413-420. DOI： 10.16016/j.2097-0927.202107181.

Zongbao YAN, Fangyuan MAO, Zhiguo SHAN, et al. Effect and mechanism of H. pylori in regulation of REDD1 expression in gastric mucosa[J]. Journal of Army Medical University, 2022, 44(5): 413-420. DOI： 10.16016/j.2097-0927.202107181.

摘要

目的

探讨DNA损伤应答蛋白1 (regulated in development and DNA damage responses-1，REDD1)在幽门螺杆菌(

Helicobacter pylori

，

H.pylori

) 感染中的表达及调控机制。

方法

建立

H.pylori

感染C57小鼠及胃上皮细胞模型，运用实时荧光定量PCR、免疫组织化学染色和Western blot检测REDD1 mRNA和蛋白的表达；并在细胞模型中采用信号通路抑制剂的方法探讨

H.pylori

感染诱导REDD1上调的机制。

结果

相对于未感染组，

H.pylori

感染小鼠胃黏膜中的REDD1水平显著增高；而相对于野生型(Wild Type，WT)全毒株，敲除cagA基因后，

H.pylori

感染诱导REDD1上调的能力则显著下降(

0.05)；

H.pylori

感染可诱导胃上皮细胞AGS REDD1表达上调，并具有时间、感染菌量以及cagA依赖性(

0.05)；P38/MAPK信号通路阻断可显著抑制

H.pylori

感染诱导的REDD1上调表达(

0.05)。

结论

H.pylori

依赖磷酸化的cagA蛋白激活MAPKp38通路诱导REDD1表达增高。

Abstract

Objective

Regulated in development and DNA damage responses-1 (REDD1) is a conserved and ubiquitous protein

and is induced in response to multiple stimuli. However

its regulation

function and clinical relevance in

Helicobacter pylori

(

H. pylori

)-associated gastritis are presently unknown. The study aimed to investigate its expression and regulation mechanism in

H. pylori

infection.

Methods

C57 mice and gastric epithelial cells (AGS cells) were respectively infected with

H. pylori

to establish infected models. Then the expression of REDD1 at mRNA and protein levels was detected by quantitative PCR

immunohistochemical staining and Western blotting. The mechanism of REDD1 up-regulation induced by

H. pylori

infection was investigated by transfection with signaling pathway inhibitor

plasmid or dual-luciferase reporting analysis in cell models.

Results

The expression of REDD1 was increased in gastric mucosa of the

H. pylori

-infected mice than the un-infected ones. Compared with the wild-type strain of

H. pylori

the strain with cagA knockout (△cagA

H. pylori

11637 cells) showed decreased effect on the upregulation of REDD1 (

0.05).

H. pylori

infection induced up-regulation of REDD1 in AGS cells in a time-

cagA- and pathogen dose-dependent m

anner (

0.05). Blocking of P38/MAPKp38 signaling pathway could significantly inhibit the upregulation of REDD1 induced by the infection (

0.05).

Conclusion

H. pylori

induces the up-regulation of REDD1 through activation of phosphorylated cagA in MAPKp38 pathway in the gastric mucosa of mouse with

H. pylori

infection.

关键词

Keywords

references

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