罗布麻提取物改善小鼠高脂饮食诱导的代谢失调与肠道菌群紊乱

杨杰; 付慧; 张晋; 夏静; 白小龙; 李佳珍; 周思艺; 刘威

doi:10.16016/j.2097-0927.202106020

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罗布麻提取物改善小鼠高脂饮食诱导的代谢失调与肠道菌群紊乱

基础医学 | 更新时间：2026-01-09

- 罗布麻提取物改善小鼠高脂饮食诱导的代谢失调与肠道菌群紊乱
- Apocynum venetum L. extract ameliorates metabolic dysbiosis and intestinal flora disorder induced by high-fat diet in mice
- 陆军军医大学学报 2022年44卷第3期页码：266-274
- 作者机构：
  
  1.032200 山西汾阳，山西医科大学汾阳学院临床医学系
  2.032200 山西汾阳，山西医科大学汾阳学院科技中心
  3.032200 山西汾阳，山西医科大学汾阳学院眼视光学系
  4.032200 山西汾阳，山西医科大学汾阳学院医学检验系
- 作者简介：
  
  [付慧，E-mail：fuhui132@163.comFU Hui, E-mail: fuhui132@163.com
  [刘威，E-mail：liuwei@sxmu.edu.cnLIU Wei, E-mail: liuwei@sxmu.edu.cn
- 基金信息：
  
  国家自然科学基金青年项目(31501175);山西医科大学汾阳学院校级大学生创新创业训练项目(FDC201905);山西医科大学汾阳学院人才引进启动基金(2019D12)
- DOI：10.16016/j.2097-0927.202106020
  中图分类号： R282.71;R372;R589.2
- 收稿：2021-06-20，
  
  修回：2021-09-26，
  
  纸质出版：2022-02-15
- 稿件说明：
移动端阅览
杨杰, 付慧, 张晋, 等. 罗布麻提取物改善小鼠高脂饮食诱导的代谢失调与肠道菌群紊乱[J]. 陆军军医大学学报, 2022,44(3):266-274.

Jie YANG, Hui FU, Jin ZHANG, et al. Apocynum venetum L. extract ameliorates metabolic dysbiosis and intestinal flora disorder induced by high-fat diet in mice[J]. Journal of Army Medical University, 2022, 44(3): 266-274.
杨杰, 付慧, 张晋, 等. 罗布麻提取物改善小鼠高脂饮食诱导的代谢失调与肠道菌群紊乱[J]. 陆军军医大学学报, 2022,44(3):266-274. DOI： 10.16016/j.2097-0927.202106020.

Jie YANG, Hui FU, Jin ZHANG, et al. Apocynum venetum L. extract ameliorates metabolic dysbiosis and intestinal flora disorder induced by high-fat diet in mice[J]. Journal of Army Medical University, 2022, 44(3): 266-274. DOI： 10.16016/j.2097-0927.202106020.

摘要

目的

探讨罗布麻(

Apocynum venetum

L.，AV)对小鼠高脂食物诱导的肥胖、代谢失调及肠道菌群紊乱的作用。

方法

将18只C57BL/6小鼠随机分为3组(

=6)：对照组(ND组，喂正常食物，正常饮水)、肥胖模型组(HFD组，喂高脂食物，正常饮水)和AV处理组(AV组，喂高脂食物，饮水添加AV提取物)。每周日称量小鼠体质量。12周结束时，称量小鼠皮下脂肪和内脏脂肪；HE染色观察肝脏脂质变性情况；运用生化试剂盒测定血清中葡萄糖、甘油三酯(triglyceride

TG)、总胆固醇(total cholesterol

TC)和低密度脂蛋白胆固醇(low-density lipoprotein cholesterol

LDL-C)水平；RT-PCR法检测小鼠肠道内IL-6、IL-1β、TNF-α和抵抗素(Resistin)等促炎因子的表达；16S rRNA基因高通量测序分析小鼠肠道菌群的变化。

结果

与HFD组相比，AV组小鼠体质量明显降低(

0.05)，内脏及皮下脂肪含量显著减少(

0.05)，血清中葡萄糖、TC、TG、LDL-C水平显著降低(

0.05)，肝脏组织脂肪变性程度减轻，肠道内促炎因子IL-6、IL-1β、TNF-α和Resistin的表达显著降低(

0.05)。高通量测序结果显示，与ND组相比，HFD组小鼠肠道菌群发生紊乱，菌群整体结构和功能发生变化；与HFD组相比，AV组中AV提取物可改善肠道菌群紊乱，逆转了高脂饮食诱导的菌群整体结构和功能的改变。

结论

AV可改善小鼠高脂诱导性肥胖及代谢失调，并减轻高脂诱导的肠道菌群紊乱。

Abstract

Objective

To investigate the effects of

Apocynum venetum

L. (AV) on obesity

metabolic dysbiosis and intestinal flora disorder induced by high-fat diet in mice.

Methods

A total of 18 C57BL/6 mice were randomly divided in

to 3 groups (

=6 in each): control group

obesity model group (HFD) and AV treatment group (AV). The mice of the control group were fed with normal diet

those from the other 2 groups with high-fat diet

and those of the last group were concurrently given drinking water containing AV extract. All mice were weighed every Sunday. After 12 weeks of intervention

the subcutaneous and visceral fat of mice were measured; the liver lipid degeneration was observed by HE staining; the serum levels of glucose

triglyceride (TG)

total cholesterol (TC) and low-density lipoprotein cholesterol (LDL-C) were tested using biochemical kits. In addition

the expression levels of IL-6

IL-1β

TNF-α and resistin in the intestinal tract were detected by RT-PCR

and the changes of intestinal microbiota were analyzed using high-throughput sequencing of 16S rRNA gene.

Results

As compared with the HFD group

the AV group showed significantly lighter body weight (

0.05)

less visceral and subcutaneous fat contents (

0.05)

and lower serum levels of glucose

TG and LDL-C (

0.05). Similarly

the steatosis of liver tissue was alleviated in the AV-treated mice

with remarkably decreased levels of pro-inflammatory factors in the intestine (

0.05). High-throughput sequencing results suggested that HFD notably induced intestinal flora disorder

including alterations of overall distribution and function of the intestinal microbiota

while these changes were reversed by AV extract treatment.

Conclusion

AV extract can ameliorate HFD-induced obesity and metabolic dysbiosis

as well as alleviate intestinal flora disorder in mice.

关键词

Keywords

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