单细胞组学视角下的心肌细胞增殖

曾春雨; 付文斌

doi:10.16016/j.1000-5404.202111168

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单细胞组学视角下的心肌细胞增殖

基础医学 | 更新时间：2026-01-09

- 单细胞组学视角下的心肌细胞增殖
- Cardiomyocyte proliferation at single-cell resolution
- 陆军军医大学学报 2022年44卷第1期页码：69-73
- 作者机构：
  
  400042 重庆，陆军特色医学中心心血管内科，重庆市心血管病研究所
- 作者简介：
  
  [曾春雨，博士，主任医师，教授，博士研究生导师，长江学者特聘教授，国家杰出青年科学基金获得者，国家中青年科技创新领军人才，国家重点研发计划、973计划项目首席科学家，国家“万人计划”领军人才，美国心脏学院杰出科学家奖获得者。担任中国医师心血管分会代谢心血管专委会副主任委员、中国生理学会整合生理专委会副主任委员、国家高血压医联体副理事长、国家高血压医联体重庆中心主任、中华医学会心血管专委会委员、重庆市医学会心血管病专业委员会主任委员等。先后获得重庆市自然科学一等奖、重庆市科技进步一等奖等5个奖项；获2021年度美国ACC杰出科学家奖、全国科技先进工作者、重庆市青年科技创新杰出奖、全国十大杰出青年提名奖、重庆市五四青年奖章等荣誉。主研的“成年心肌增殖”工作被评为“中国心血管领域十大进展、十大影响力事件”、被Nat Rev Cardiol评为年度里程碑影响；“GRK4在高血压中的研究作用”成果被美国高血压学会评为2015年年度最具转化前景的工作，并被美国NIH的STTR项目委员会推荐为年度具有研发前景的12个项目之一。担任Clin Exp Hypertens、Hypertension(中文版)和《中华高血压杂志》副主编、SCI收录的国外20余种期刊编委。在Circulation、Circ Res、Eur Heart J等SCI收录的期刊发表160余篇。主要从事高血压病和心肌再生的基础和临床方面的研究。E-mail: chunyuzeng01@163.com ZENG Chunyu, E-mail: chunyuzeng01@163.com
- 基金信息：
  
  国家自然科学基金重点项目(81930008)
- DOI：10.16016/j.1000-5404.202111168
  中图分类号： R322.11;R329.28;R394.2
- 收稿：2021-11-23，
  
  修回：2021-12-07，
  
  纸质出版：2022-01-15
- 稿件说明：
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曾春雨, 付文斌. 单细胞组学视角下的心肌细胞增殖[J]. 陆军军医大学学报, 2022,44(1):69-73.

Chunyu ZENG, Wenbin FU. Cardiomyocyte proliferation at single-cell resolution[J]. Journal of Army Medical University, 2022, 44(1): 69-73.
曾春雨, 付文斌. 单细胞组学视角下的心肌细胞增殖[J]. 陆军军医大学学报, 2022,44(1):69-73. DOI： 10.16016/j.1000-5404.202111168.

Chunyu ZENG, Wenbin FU. Cardiomyocyte proliferation at single-cell resolution[J]. Journal of Army Medical University, 2022, 44(1): 69-73. DOI： 10.16016/j.1000-5404.202111168.

摘要

单细胞多组学测序技术是在单细胞分辨率下精确分析细胞异质性和基因调控网络的新技术，提供了全新的视角来分析细胞的分化路径、细胞间的交互调控和细胞亚群的分离现象。心肌再生与修复是逆转心肌损伤后重构的重要研究方向。目前，单细胞多组学测序技术已应用于心肌再生领域：构建生理和病理条件下心肌再生图谱，探索心肌细胞增殖的分化路径和非心肌细胞对心肌再生的交互作用，建立全新的连续细胞周期分子标志监测的心肌细胞增殖评价体系以及寻找调控心肌细胞增殖的分子开关等，具有广阔的前景。本述评总结了近几年心肌再生领域的最新研究和进展，探讨单细胞技术在心肌再生领域研究中的应用及今后研究的方向，以期为后续研究提供积极合理的参考。

Abstract

Single-cell multi-omics sequencing is a new technology to accurately analyze cell heterogeneity and gene regulatory network at single-cell resolution

and provides a brand new perspective of pathway analysis on cell differentiation

interaction and heterogeneity. Cardiac regeneration is an important research direction for reversing myocardial injury and remodeling. At present

single-cell omics sequencing technologies have been wildly applied in the field of cardiac regeneration. Great achievements and prospects have been made in the following aspects: landscape of cardiomyocyte proliferation under physiological and pathological conditions; cardiomyocyte differentiation trajectory and interaction of non-cardiomyocytes on cardiac regeneration; new evaluation system for cardiomyocyte proliferation based on consecutively monitoring cell cycle molecular markers; molecular switch regulating cardiomyocyte proliferation. This review summarizes the latest research and progress in the field of cardiac regeneration and discusses the application and development direction of single-cell technologies in the field in order to provide positive and reasonable reference for the future research.

关键词

Keywords

references

LI Y, HE L, HUANG X, et al . Genetic lineage tracing of nonmyocyte population by dual recombinases[J ] . Circulation, 2018, 138(8): 793-805. DOI: 10.1161/circulationaha.118.034250.

VAN BERLO J H, KANISICAK O, MAILLET M, et al . C-kit+ cells minimally contribute cardiomyocytes to the heart[J ] . Nature, 2014, 509(7500): 337-341. DOI: 10.1038/nature13309.

LIU Q Z, YANG R, HUANG X Z, et al . Genetic lineage tracing identifies in situ Kit-expressing cardiomyocytes[J ] . Cell Res, 2016, 26(1): 119-130. DOI: 10.1038/cr.2015.143.

HE L, LI Y, LI Y, et al . Enhancing the precision of genetic lineage tracing using dual recombinases[J ] . Nat Med, 2017, 23(12): 1488-1498. DOI: 10.1038/nm.4437.

JOPLING C, SLEEP E, RAYA M, et al . Zebrafish heart regeneration occurs by cardiomyocyte dedifferentiation and proliferation[J ] . Nature, 2010, 464(7288): 606-609. DOI: 10.1038/nature08899.

KUBIN T, PÖLING J, KOSTIN S, et al . Oncostatin M is a major mediator of cardiomyocyte dedifferentiation and remodeling[J ] . Cell Stem Cell, 2011, 9(5): 420-432. DOI: 10.1016/j.stem.2011.08.013.

WANG W E, LI L, XIA X, et al . Dedifferentiation, proliferation, and redifferentiation of adult mammalian cardiomyocytes after ischemic injury[J ] . Circulation, 2017, 136(9): 834-848. DOI: 10.1161/circulationaha.116.024307.

CHEN Y, LIU Y, GAO X. The application of single-cell technologies in cardiovascular research[J]. Front Cell Dev Biol, 2021, 9: 751371. DOI: 10.3389/fcell.2021.751371.

NATARAJAN K N. Single-cell tagged reverse transcription (STRT-seq)[J]. Methods Mol Biol, 2019, 1979: 133-153. DOI: 10.1007/978-1-4939-9240-9_9.

BAGERITZ J, RADDI G. Single-cell RNA sequencing with drop-seq[J]. Methods Mol Biol, 2019, 1979: 73-85. DOI: 10.1007/978-1-4939-9240-9_6.

HAGEMANN-JENSEN M, ZIEGENHAIN C, CHEN P, et al . Single-cell RNA counting at allele and isoform resolution using Smart-seq3[J ] . Nat Biotechnol, 2020, 38(6): 708-714. DOI: 10.1038/s41587-020-0497-0.

BUENROSTRO J D, WU B, CHANG H Y, et al . ATAC-seq: a method for assaying chromatin accessibility genome-wide[J ] . Curr Protoc Mol Biol, 2015, 109: 21.29.1-21.29.9. DOI: 10.1002/0471142727.mb2129s109.

CHEUNG R K, UTZ P J. Screening: CyTOF-the next generation of cell detection[J]. Nat Rev Rheumatol, 2011, 7(9): 502-503. DOI: 10.1038/nrrheum.2011.110.

SHI Q, QIN L, WEI W, et al . Single-cell proteomic chip for profiling intracellular signal ing pathways in single tumor cells[J ] . Proc Natl Acad Sci U S A, 2012, 109(2): 419-424.DOI: 10.1073/pnas.1110865109.

PORRELLO E R, MAHMOUD A I, SIMPSON E, et al . Transient regenerative potential of the neonatal mouse heart[J ] . Science, 2011, 331(6020): 1078-1080. DOI: 10.1126/science.1200708.

CUI M, WANG Z, CHEN K, et al . Dynamic transcriptional responses to injury of regenerative and non-regenerative cardiomyocytes revealed by single-nucleus RNA sequencing[J ] . Dev Cell, 2020, 53(1): 102-116. e8. DOI: 10.1016/j.devcel.2020.02.019.

WANG Z, CUI M, SHAH A M, et al . Cell-type-specific gene regulatory networks underlying murine neonatal heart regeneration at single-cell resolution[J ] . Cell Rep, 2020, 33(10): 108472. DOI: 10.1016/j.celrep.2020.108472.

HU P, LIU J, ZHAO J, et al . Single-nucleus transcriptomic survey of cell diversity and functional maturation in postnatal mammalian hearts[J ] . Genes Dev, 2018, 32(19/20): 1344-1357. DOI: 10.1101/gad.316802.118.

WANG Y, YAO F, WANG L, et al . Single-cell analysis of murine fibroblasts identifi es neonatal to adult switching that regulates cardiomyocyte maturation[J ] . Nat Commun, 2020, 11(1): 2585. DOI: 10.1038/s41467-020-16204-w.

HONKOOP H, DE BAKKER DE, AHARONOV A, et al . Single-cell analysis uncovers that metabolic reprogramming by ErbB2 signaling is essential for cardiomyocyte proliferation in the regenerating heart[J ] . Elife, 2019, 8: e50163. DOI: 10.7554/eLife.50163.

POTTER S S. Single-cell RNA sequencing for the study of development, physiology and disease[J]. Nat Rev Nephrol, 2018, 14(8): 479-492. DOI: 10.1038/s41581-018-0021-7.

GAO C, ZHANG M, CHEN L. The comparison of two single-cell sequencing platforms: BD rhapsody and 10×genomics chromium[J]. Curr Genomics, 2020, 21(8): 602-609. DOI: 10.2174/1389202921999200625220812.

FU W B, LIAO Q, LI L P, et al . An aurora kinase B-based mouse system to efficiently identify and analyze proliferating cardiomyocytes[J ] . Front Cell Dev Biol, 2020, 8: 570252. DOI: 10.3389/fcell.2020.570252.

PICELLI S, BJÖRKLUND Å K, FARIDANI O R, et al . Smart-seq2 for sensitive full-length transcriptome profiling in single cel ls[J ] . Nat Methods, 2013, 10(11): 1096-1098. DOI: 10.1038/nmeth.2639.

WANG L, YU P, ZHOU B, et al . Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function[J ] . Nat Cell Biol, 2020, 22(1): 108-119. DOI: 10.1038/s41556-019-0446-7.

付文斌, 王伟, 曾春雨. 心肌细胞增殖影响因素和评价方法[J]. 中国科学: 生命科学, 2021, 1(1): 1-13. DOI: 10.1360/SSV-2020-0327.

FU W B, WANG W, ZENG C Y. Influencing factors and evaluation methods of cardiomyocyte proliferation[J]. Sci Sin Vitae, 2021, 1(1): 1-13. DOI: 10.1360/SSV-2020-0327.

HIRAI M, CHEN J, EVANS S M. Tissue-specific cell cycle indicator reveals unexpected findings for cardiac myocyte proliferation[J]. Circ Res, 2016, 118(1): 20-28. DOI: 10.1161/circresaha.115.307697.

KRETZSCHMAR K, POST Y, BANNIER-HÉLAOUËT M, et al . Profiling proliferative cells and their progeny in damaged murine hearts[J ] . Proc Natl Acad Sci USA, 2018, 115(52): E12245-E12254. DOI: 10.1073/pnas.1805829115.

HESSE M, DOENGI M, BECKER A, et al . Midbody positioning and distance between daughter nuclei enable unequivocal identification of cardiomyocyte cell division in mice[J ] . Circ Res, 2018, 123(9): 1039-1052. DOI: 10.1161/circresaha.118.312792.

ALI S R, HIPPENMEYER S, SAADAT L V, et al . Existing cardiomyocytes generate cardiomyocytes at a low rate after birth in mice[J ] . Proc Natl Acad Sci USA, 2014, 111(24): 8850-8855. DOI: 10.1073/pnas.1408233111.

LIU X X, PU W J, HE L J, et al . Cell proliferation fate mapping reveals regional cardiomyocyte cell-cycle activity in subendocardial muscle of left ventricle[J ] . Nat Commun, 2021, 12(1): 5784. DOI: 10.1038/s41467-021-25933-5.

ASP M, GIACOMELLO S, LARSSON L, et al . A spatiotemporal organ-wide gene expression and cell atlas of the developing human heart[J ] . Cell, 2019, 179(7): 1647-1660. e19. DOI:10.1016/j.cell.2019.11.025.

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