皮肤优势型慢性移植物抗宿主病小鼠模型的构建

王蔚浩; 杨世杰; 杜雨軒; 王筱淇; 洪涛; 何云地; 王瑞; 马超; 安慧慧; 王俊洁; 张云芳; 高强国; 张曦

doi:10.16016/j.1000-5404.202108060

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皮肤优势型慢性移植物抗宿主病小鼠模型的构建

专题报道：造血干细胞移植并发症的基础与临床研究 | 更新时间：2026-01-09

- 皮肤优势型慢性移植物抗宿主病小鼠模型的构建
- Establishment of a mouse model of skin-dominant chronic graft-versus-host disease
- 第三军医大学学报 2021年43卷第21期页码：2286-2293
- 作者机构：
  
  1.400037 重庆，陆军军医大学(第三军医大学)第二附属医院血液病医学中心，全军血液病中心，创伤、烧伤与复合伤国家重点实验室
  2.563099 贵州遵义，遵义医科大学第二附属医院检验科
  3.400038 重庆，陆军军医大学(第三军医大学)基础医学院细胞生物学教研室
- 作者简介：
  
  [张曦，E-mail: zhangxxi@sina.com ZHANG Xi, E-mail: zhangxxi@sina.com
- 基金信息：
  
  国家重点研究计划重点专项(2017YFA0105502);国家血液系统疾病临床医学研究中心2020年度开放课题重点项目(2020ZKZC02);军事医学基础前沿领域专项(2018YQYLY002)
- DOI：10.16016/j.1000-5404.202108060
  中图分类号： R-332;R617;R733.7
- 收稿：2021-08-10，
  
  修回：2021-09-05，
  
  纸质出版：2021-11-15
- 稿件说明：
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王蔚浩, 杨世杰, 杜雨軒, 等. 皮肤优势型慢性移植物抗宿主病小鼠模型的构建[J]. 第三军医大学学报, 2021,43(21):2286-2293.

Weihao WANG, Shijie YANG, Yuxuan DU, et al. Establishment of a mouse model of skin-dominant chronic graft-versus-host disease[J]. Journal of Third Military Medical University, 2021, 43(21): 2286-2293.
王蔚浩, 杨世杰, 杜雨軒, 等. 皮肤优势型慢性移植物抗宿主病小鼠模型的构建[J]. 第三军医大学学报, 2021,43(21):2286-2293. DOI： 10.16016/j.1000-5404.202108060.

Weihao WANG, Shijie YANG, Yuxuan DU, et al. Establishment of a mouse model of skin-dominant chronic graft-versus-host disease[J]. Journal of Third Military Medical University, 2021, 43(21): 2286-2293. DOI： 10.16016/j.1000-5404.202108060.

摘要

目的

构建皮肤优势型慢性移植物抗宿主病(chronic graft-versus-host disease

cGVHD)小鼠模型。

方法

利用致死剂量辐照BALB/c小鼠，并接受来自B10.D2供鼠混合的骨髓细胞和脾脏细胞以构建皮肤优势型cGVHD小鼠模型，通过小鼠临床表现、病理特征及靶器官细胞亚群评估模型构建情况。

结果

模型小鼠的临床表现以皮肤脱毛、硬化、皮屑、结痂和由皮肤皱缩硬化引起的弓背和关节僵直为主，临床病理评分随疾病进展升高，但无明显肠道cGVHD表现。病理改变中皮肤出现明显胶原沉积，毛囊减少甚至消失，而其他靶器官病理改变不明显。对以上靶器官行T细胞亚群分析，发现Th1细胞、CD4

IL-13

T细胞和Tc1细胞与疾病进展密切。

结论

成功构建可用于实验研究的皮肤优势型小鼠cGVHD模型，病理变化主要以皮肤纤维化表现为主。

Abstract

Objective

To construct a mouse model of skin-dominant chronic graft-versus-host disease (cGVHD).

Methods

BALB/c mice were irradiated with lethal dose and then received a mixture of bone marrow and spleen cells transplantation from B10.D2 donor mice. Then the clinical manifestations

pathological features of the mice and cell subsets of targeted organs were observed to evaluate the establishment of model.

Results

The clinical manifestations of the model mice mainly involved skin depilation

sclerosis

dandruff

scab

as well as arched back and joint stiffness due to skin shrinkage and

sclerosis. The clinicopathological scores were increased with the progression of cGVHD

while the model mice showed no obvious manifestations of intestinal cGVHD. Moreover

increase of collagen deposition and decrease or even disappearance of hair follicles was observed on the histopathologic sections of skin

while other targeted organs showed no major alterations. T cell subsets analysis indicated that Th1 cells

CD4

IL-13

T cells and Tc1 cells were closely related to the disease progression.

Conclusion

The skin-dominant cGVHD mouse model is successfully established

with skin damage and fibrosis as main pathological changes.

关键词

Keywords

references

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