热休克蛋白27调控RhoA在大鼠膀胱出口梗阻致平滑肌重塑中的作用

廖德洋; 王永权; 熊智勇; 周晓洲; 何鹏; 周占松

doi:10.16016/j.1000-5404.202107073

您当前的位置：

首页 >

文章列表页 >

热休克蛋白27调控RhoA在大鼠膀胱出口梗阻致平滑肌重塑中的作用

基础医学 | 更新时间：2026-01-09

- 热休克蛋白27调控RhoA在大鼠膀胱出口梗阻致平滑肌重塑中的作用
- Role of HSP27 regulating RhoA in bladder smooth muscle remodeling in rats after bladder outlet obstruction
- 第三军医大学学报 2021年43卷第23期页码：2584-2589
- 作者机构：
  
  400038 重庆，陆军军医大学(第三军医大学)第一附属医院泌尿外科
- 作者简介：
  
  [周占松，E-mail：zhouzhansong@sohu.com ZHOU Zhansong, E-mail: zhouzhansong@sohu.com
- 基金信息：
  
  重庆市自然科学基金(cstc2018jcyjAX0278)
- DOI：10.16016/j.1000-5404.202107073
  中图分类号： R341;R363.22;R694.2
- 收稿：2021-07-11，
  
  修回：2021-09-06，
  
  纸质出版：2021-12-15
- 稿件说明：
移动端阅览
廖德洋, 王永权, 熊智勇, 等. 热休克蛋白27调控RhoA在大鼠膀胱出口梗阻致平滑肌重塑中的作用[J]. 第三军医大学学报, 2021,43(23):2584-2589.

Deyang LIAO, Yongquan WANG, Zhiyong XIONG, et al. Role of HSP27 regulating RhoA in bladder smooth muscle remodeling in rats after bladder outlet obstruction[J]. Journal of Third Military Medical University, 2021, 43(23): 2584-2589.
廖德洋, 王永权, 熊智勇, 等. 热休克蛋白27调控RhoA在大鼠膀胱出口梗阻致平滑肌重塑中的作用[J]. 第三军医大学学报, 2021,43(23):2584-2589. DOI： 10.16016/j.1000-5404.202107073.

Deyang LIAO, Yongquan WANG, Zhiyong XIONG, et al. Role of HSP27 regulating RhoA in bladder smooth muscle remodeling in rats after bladder outlet obstruction[J]. Journal of Third Military Medical University, 2021, 43(23): 2584-2589. DOI： 10.16016/j.1000-5404.202107073.

摘要

目的

探讨热休克蛋白27(heat shock protein 27，HSP27)调控RhoA在大鼠膀胱出口梗阻(bladder outlet obstruction，BOO)致平滑肌重塑中的作用及其机制。

方法

采用透明质酸尿道口注射法制作大鼠膀胱出口梗阻模型(BOO组)，对照组大鼠注射相同剂量生理盐水。4周后行膀胱组织HE染色，Western blot检测大鼠膀胱组织中HSP27及RhoA表达水平；建立人膀胱平滑肌细胞HSP27过表达(过表达组)及敲低(敲低组)模型，以转染相应空载体细胞作为对照组，Western blot检测各组HSP27、pHSP27、RhoA及G-actin、F-actin蛋白水平。F-actin鬼笔环肽荧光染色观察各组细胞中肌动蛋白结构改变。

结果

成功建立BOO大鼠模型，4周后HE染色可见BOO组大鼠膀胱平滑肌明显肥厚；BOO组大鼠膀胱组织中HSP27及RhoA表达较对照组明显上调(

0.05)。成功建立HSP27过表达及敲低模型，Western blot结果显示，与对照组比较，HSP27过表达组HSP27、p-HSP27及RhoA表达水平明显上调(

0.05)；HSP27敲低组HSP27、p-HSP27及RhoA表达水平均明显下调(

0.05)；HSP27过表达组F-actin/G-actin(F/G)比值明显上升(

0.05)，而敲低组F/G比值明显降低(

0.05)；鬼笔环肽染色结果显示，HSP27敲低组平滑肌细胞内肌动蛋白丝含量较对照组和过表达组明显减少，HSP27过表达组平滑肌细胞内肌动蛋白丝含量较对照组明显增多，排列紊乱。

结论

HSP27可通过调控RhoA，影响膀胱平滑肌细胞内肌动蛋白结构，可能在大鼠膀胱出口梗阻后膀胱平滑肌重塑中发挥重要作用。

Abstract

Objective

To explore the role and underlying mechanism of heat shock protein 27 (HSP27) in bladder smooth muscle remodeling after bladder outlet obstruction (BOO).

Methods

Rat BOO model was established by urethral orifice hyaluronic acid injection

and the rats from the control group were given the injection of same amount of normal saline. In 4 weeks after model establishment

the bladder wall tissues of BOO and control group were observed by HE staining. The expression of HSP27 and RhoA was detected by Western blotting in both groups. Cultured human bladder smooth muscle cells (BSMCs) were transfected with lentiviral vectors expressing either HSP27 or HSP27-shRNA (shHSP27). Western blotting was used to detect the expression of HSP27

phosphorylated HSP27

RhoA

G-actin and F-actin. Phalloidin fluorescence staining of F-actin was performed in overexpression and control group to observe actin filaments in BSMCs.

Results

HE staining showed evident bladder smooth muscle hypertrophy in BOO group in 4 weeks after hyaluronic acid injection

indicating the successful establishment of rat BOO model. The expression levels of HSP27 and RhoA were increased significantly compared to those in the control group (

0.05). BMSC cell models of HSP27 overexpression and knockdown were successfully established. The Results of Western blotting showed increased expression of HSP27

phosphorylated HSP27 and RhoA in HSP27 overexpression cells (

0.05)

and decreased expression of these protein in shHSP27 cells (

0.05). Also

the F-actin/G-actin ratio was elevated in HSP27 overexpression cells (

0.05) and an opposite result was seen in the shHSP27 cells (

0.05). Phalloidin fluorescence staining showed there were less actin filaments in shHSP27 cells than the control and HSP27 overexpression cells

and more actin filaments in abnormal arrangement were observed in the overexpression cells than the control cells.

Conclusion

HSP27 can affect the structure of actin by regulating RhoA

and may play a critical role in bladder smooth muscle remodeling after BOO in rats.

关键词

Keywords

references

D'ANCONA C, HAYLEN B, OELKE M, et al . The International Continence Society (ICS) report on the terminology for adult male lower urinary tract and pelvic floor symptoms and dysfunction[J ] . Neurourol Urodyn, 2019, 38(2): 433-477. DOI: 10.1002/nau.23897.

FUSCO F, CRETA M, DE NUNZIO C, et al . Progressive bladder remodeling due to bladder outlet obstruction: a systematic review of morphological and molecular evidences in humans[J ] . BMC Urol, 2018, 18(1): 15. DOI: 10.1186/s12894-018-0329-4.

KANNO Y, MITSUI T, KITTA T, et al . The inflammatory cytokine IL-1β is involved in bladder remodeling after bladder outlet obstruction i n mice[J ] . Neurourol Urodyn, 2016, 35(3): 377-381. DOI: 10.1002/nau.22721.

王永权, 沈文浩, 邓建平, 等. 热休克蛋白27在大鼠急性尿潴留膀胱中的表达及意义[J]. 局解手术学杂志, 2011, 20(2): 124-126. DOI: 10.3969/j.issn.1672-5042.2011.02.005.

WANG Y Q, SHEN W H, DENG J P, et al . Expressions and significances of heat shock protein 27 in bladders of rats with acute urinary retention[J ] . J Reg Anat Oper Surg, 2011, 20(2): 124-126. DOI: 10.3969/j.issn.1672-5042.2011.02.005.

NARUMIYA S, THUMKEO D. Rho signaling research: history, current status and future directions[J]. FEBS Lett, 2018, 592(11): 1763-1776. DOI: 10.1002/1873-3468.13087.

ROTTNER K, FAIX J, BOGDAN S, et al . Actin assembly mechanisms at a glance[J ] . J Cell Sci, 2017, 130(20): 3427-3435. DOI: 10.1242/jcs.206433.

LEE S H, DOMINGUEZ R. Regulation of actin cytoskeleton dynamics in cells[J]. Mol Cells, 2010, 29(4): 311-325. DOI: 10.1007/s10059-010-0053-8.

WANG Y Q, XIONG Z Y, GONG W, et al . Urethral orifice hyaluronic acid injections: a novel animal model of bladder outlet obstruction[J ] . BMC Urol, 2015, 15 : 8. DOI: 10.1186/s12894-015-0002-0.

RIDLEY A J. Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking[J]. Trends Cell Biol, 2006, 16(10): 522-529. DOI: 10.1016/j.tcb.2006.08.006.

SCHAKS M, GIANNONE G, ROTTNER K. Actin dynamics in cell migration[J]. Essays Biochem, 2019, 63(5): 483-495. DOI: 10.1042/ebc20190015.

BLANCHOIN L, BOUJEMAA-PATERSKI R, SYKES C, et al . Actin dynamics, architecture, and mechanics in cell motility[J ] . Physiol Rev, 2014, 94(1): 235-263. DOI: 10.1152/physrev.00018.2013.

TANG D D. Critical role of actin-associated proteins in smooth muscle contraction, cell proliferation, airway hyperresponsiveness and airway remodeling[J]. Respir Res, 2015, 16: 134. DOI: 10.1186/s12931-015-0296-1.

ZHANG Y, SARADNA A, RATANR, et al . RhoA/Rho-kinases in asthma: from pathogenesis to therapeutic targets[J ] . Clin Transl Immunology, 2020, 9(5): e01134. DOI: 10.1002/cti2.1134.

STRASSHEIM D, GERASIMOVSKAYA E, IRWIN D, et al . RhoGTPase in vascular disease[J ] . Cell, 2019, 8(6): 551. DOI: 10.3390/cells8060551.

HIGASHIDA C, KIUCHI T, AKIBA Y, et al . F- and G-actin homeostasis regulates mechanosensitive actin nucleation by formins[J ] . Nat Cell Biol, 2013, 15(4): 395-405. DOI: 10.1038/ncb2693.

DUAN X, ZHANG Y, CHEN K L, et al . The small GTPase RhoA regulates the LIMK1/2-cofilin pathway to modulate cytoskeletal dynamics in oocyte meiosis[J ] . J Cell Physiol, 2018, 233(8): 6088-6097. DOI: 10.1002/jcp.26450.

WANG R X, YU Q F, WANG X L, et al . Rac1 silencing, NSC23766 and EHT1864 reduce growth and actin organization of bladder smooth muscle cells[J ] . Life Sci, 2020, 261: 118468. DOI: 10.1016/j.lfs.2020.118468.

OH M M, CHOI H, PARK M G, et al . Is there a correlation between the presence of idiopathic detrusor overactivity and the degree of bladder outlet obstruction?[J ] . Urology, 2011, 77(1): 167-170. DOI: 10.1016/j.urology.2010.05.034.

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

外源性干细胞因子对逼尿肌活动低下大鼠Cajal间质细胞数及逼尿肌收缩力的影响

静态尿道压力图对良性前列腺增生所致膀胱出口梗阻的诊断价值

半坐卧位对良性前列腺增生患者压力-流率测定的影响

脑缺血再灌注大鼠GAP-43和RhoA蛋白表达及NEP1-40对其的影响

调控RhoA/Rac平衡对失血性休克大鼠血管双相反应性的影响